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United States Department of Agriculture

Agricultural Research Service

Title: Introgression of Resistance to Late Blight from Wild Species Using Embryo Rescue and Double Pollination

item Hanneman Jr, Robert

Submitted to: American Journal of Potato Research
Publication Type: Abstract Only
Publication Acceptance Date: June 28, 1996
Publication Date: N/A

Technical Abstract: Incorporation of late blight resistance from the wild tuber-bearing Solanum species into the cultivated potato, Solanum tuberosum Gp. Tuberosum, is very important; however, the success of introgression is dependent on interspecific crossability. While the majority of diploid species hybridize readily with Tuberosum haploids, some 2x(1EBN) species do not cross with 4x(4EBN) or 2x(2EBN) Tuberosum. Extensive crossing, manipulation of EBN, and embryo rescue with double pollination (second compatible pollination) were used to overcome these barriers. EBN was used as a guide to create a crossing scheme. A total of 122 late blight resistant PIs representing 31 species, 82 haploids and 14 cultivars were used. During the spring and summer of 1995, 9,463 pollinations, of which 3,077 were from haploid-2x(1EBN) species crosses, were made to transfer late blight resistance from selected wild species to cultivated potatoes. These crosses yielded 219 fruits, and 692 seeds. Embryo rescue was conducted on 40 of these fruits from the 2x(1EBN) crosses yielding 329 embryos, and resulting in 88 seedlings; however, no viable embryos were obtained with 2x(1EBN) species as females. The accessions used were tested for late blight resistance using the detached leaf method. Chromosome counting and screening for resistance are in progress.

Last Modified: 4/22/2015
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