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United States Department of Agriculture

Agricultural Research Service

Title: Cytokine Expression in Lymph Nodes Infected with Mycobacterium Bovis

Authors
item Brown, C - UNIV. OF GA
item Whipple, Diana
item Bolin, Carole
item Rhyan, J - USDA/APHIS/NVSL
item Saari, D - USDA/APHIS/NVSL
item Miller, Janice

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: November 16, 1997
Publication Date: N/A

Technical Abstract: Retropharyngeal lymph nodes were taken from animals 6 and 8 weeks after instillation of 4.8 x 10*5 Mycobacterium bovis into the crypts of the palatine tonsils. Tissues were routinely processed into paraffin blocks and sections were probed for the presence of tumor necrosis factor (TNF)-al interleukin (IL)-10, and gamma-interferon (gamma-IFN) mRNA using digoxigenin-labeled riboprobes in an in situ hybridization procedure. In infected animals, TNF-alpha was present in abundant quantities in macrophages surrounding granulomas, however there was no TNF-alpha in epithelioid macrophages or multinucleate giant cells. IL-10 was present in lymphocyte-like cells, often in the compressed lymphoid tissue adjacent to a granuloma. Staining for gamma-IFN was very weak.

Last Modified: 4/20/2014
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