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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Ruminant Diseases and Immunology Research » Research » Publications at this Location » Publication #86555
Title: REGULATION OF NEUTROPHIL AND MONONUCLEAR ADHESION MOLECULES IN CALVES TREATED WITH VARIOUS NON-STEROIDAL ANTI-INFLAMMATORY DRUGS (NSAIDS)

Author
item PELAN-MATTOCKS, LISA - IOWA STATE UNIV., AMES
item Kehrli Jr, Marcus

Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 11/10/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: NSAIDs are used in cattle for symptomatic treatment of acute mastitis, diarrhea, pneumonia, and other conditions of excessive inflammation, endotoxemia, or pain. NSAIDs typically interfere with the cyclo-oxygenase pathway of arachidonic acid metabolism in cells and possess other pharmacological effects resulting in suppressed leukocyte function, including altered neutrophil adhesion molecule expression. Adhesion molecules on endothelial cells and leukocytes mediate adhesion reactions leading to leukocyte egress during inflammation. We assessed by flow cytometry the effects of a single dose of selected NSAIDs and a glucocorticoid, dexamethasone, on circulating gamma-delta T-cells and leukocyte adhesion molecules (CD18, CD62L, CD11a, CD11b, CD11c). Leukograms and neutrophil functions (iodination and bacterial ingestion) were also assessed on 22 calves. Dexamethasone down-regulated CD18 and CD62L; however, ibuprofen, phenylbutazone, flunixin meglumine, and aspirin did not elicit down-regulation of CD62L nor an accompanying neutrophilia. No consistent suppression of neutrophil ingestion of bacteria or iodination was observed with any of the drugs. CD18 was up-regulated significantly by aspirin, ibuprofen, and flunixin meglumine. Aspirin also increased the percentage of gamma-delta T-cells. The anti-inflammatory mechanisms of these NSAIDs do not impair leukocyte surface adhesion molecule function and are not likely to alter leukocyte trafficking.