Author
KANG, JIE - Arkansas Children'S Nutrition Research Center (ACNC) | |
LI, ZHIMIN - Shanghai Institute Of Pharmaceutical Industry | |
WU, TONG - Shanghai Institute Of Pharmaceutical Industry | |
JENSEN, GITTE - Nis Labs | |
SCHAUSS, ALEXANDER - Aibmr Life Sciences (AMERICAN INSTITUTE FOR BIOSOCIAL AND MEDICAL RESEARCH) | |
WU, XIANLI - Arkansas Children'S Nutrition Research Center (ACNC) |
Submitted to: Journal of Federation of American Societies for Experimental Biology
Publication Type: Abstract Only Publication Acceptance Date: 3/15/2010 Publication Date: 4/12/2010 Citation: Kang, J., Li, Z., Wu, T., Jensen, G.S., Schauss, A.G., Wu, X. 2010. Antioxidant capacities of seven flavonoid compounds isolated from pulp of acai fruit (Euterpe oleracea) [abstract]. Journal of Federation of American Societies for Experimental Biology. Journal of Federation of American Societies for Experimental Bilogy. 24:921.8. Interpretive Summary: Technical Abstract: The pulp of açai fruit (Euterpe oleracea Mart.) has been demonstrated to exhibit extremely high antioxidant capacity. Seven major flavonoids were isolated from freeze-dried acai pulp by various chromatographic methods. Their structures were elucidated as orientin (1), homoorientin (2), vitexin (3), luteolin (4), chrysoeriol (5), quercetin (6), and dihydrokaempferol (7) by NMR, MS and compared to authentic standards. Antioxidant capacities of these flavonoids were evaluated by the following assays: oxygen radical absorbance capacity (ORAC); cell-based antioxidant protection in erythrocyte (CAP-e); ROS formation in polymorphonuclear granulocytes (ROS PMN). ORAC values varied widely (1,423.6 – 14,817.4 micro-mol TE/g) among the seven compounds based on numbers and positions of hydroxyl groups and/or substitute groups. The ORAC values of aglycones were generally higher than that of glycosides. CAP-e results indicated that three compounds (4, 6, 7) could enter the cytosol and significantly reduce oxidative damage within living cells. ROS PMN assay showed four compounds (2, 3, 7, 6) had exceptional anti-inflammatory properties in reducing ROS formation in PMN cells, even when high amounts of ROS were produced upon hydrogen peroxide administration. |