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ARS Home » Midwest Area » Madison, Wisconsin » Vegetable Crops Research » Research » Publications at this Location » Publication #339908
Title: Molecular determinants of resistance to Verticillium dahliae in potato

Author
item KUMAR, ARUN - University Of Wisconsin
item Jansky, Shelley
item ENDELMAN, JEFFREY - University Of Wisconsin
item Halterman, Dennis

Submitted to: American Journal of Potato Research
Publication Type: Proceedings
Publication Acceptance Date: 4/7/2017
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: A constant evolutionary arms race between host resistance genes and pathogen effectors determine adaptive fitness. Therefore, identification of both host resistance genes and pathogen effectors is important in devising effective strategies to control disease. In tomato, resistance to Verticillium dahliae is governed by the Ve gene, a major dominant gene that has been exploited in plant breeding programs for over 50 years. This gene recognizes the Ave effector produced by the Race 1 strain of V. dahliae and triggers a downstream defense response. However, the genetic determinants of resistance to potato against V. dahliae are still unknown due in part to complex tetraploid genetics and difficulties in disease phenotyping. Also, little is known about virulence mechanisms for potato isolates of V. dahliae. We have developed an F2 mapping population derived from the homozygous diploid parents, S. tuberosum DM1-3 (susceptible to VW) and S. chacoense M6 (resistant to VW). This population was SNP genotyped using the SolCAP array and phenotyped using a rooted cutting protocol developed in our lab. A QTL on chromosome 1 was identified which explains 31% of phenotypic variation. Using SNP marker data, 22 genes were found in the QTL region. Based on bioinformatics analysis, two possible resistance associated genes with a serine/threonine-protein kinase domain were sequenced and are being subjected to further functional validation studies. On the pathogen side, an inability to identify the Ave effector gene from V. dahliae pathogenic on potato suggests that another effector may be involved in eliciting resistance in potato hosts.