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Research Project: Prevention of Obesity Related Metabolic Diseases by Bioactive Components of Food Processing Waste Byproducts and Mitigation of Food Allergies

Location: Healthy Processed Foods Research

Title: Anti-obesity and other health benefits of bioprocessed black rice bran in combination with green tea extract in 3T3-L1 preadypocyte cells and in ice on high fat diet

Author
item KWON, KI SUN - Str Biotech Co Ltd
item LEE, EUN SEOK - Str Biotech Co Ltd
item LEE, KYUNG HEE - Str Biotech Co Ltd
item HWANG, WOON SANG - Str Biotech Co Ltd
item LEE, WHA YOUNG - Str Biotech Co Ltd
item KIM, JEANMAN - Str Biotech Co Ltd
item LEE, SANG JONG - Str Biotech Co Ltd
item KIM, SUNG PHIL - Str Biotech Co Ltd
item Friedman, Mendel

Submitted to: Food and Function
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/29/2024
Publication Date: 11/1/2024
Citation: Kwon, K., Lee, E., Lee, K., Hwang, W., Lee, W., Kim, J., Lee, S., Kim, S., Friedman, M. 2024. Anti-obesity and other health benefits of bioprocessed black rice bran in combination with green tea extract in 3T3-L1 preadypocyte cells and in ice on high fat diet. Food and Function. 15:12083-12100. https://doi.org/10.1039/D4FO03210A.
DOI: https://doi.org/10.1039/D4FO03210A

Interpretive Summary: Black rice bran, a waste product produced during the milling process of black rice which removes the bran and germ and leaves the starchy endosperm, contains biologically active anthocyanins, phenolic compounds, and phytosteroids that may be responsible for reported anti-inflammatory activities resulting in health-promoting anti-obesity and other health benefits. The bioprocessed black rice bran used in this study is a fermentation product of black rice bran using shiitake mushroom mycelia in culture. The results of the present study show that mice on a high-fat diet consuming bioprocessed black rice bran with added green tea extract experienced synergistic reduction in weight gain during the 8-week high-fat feeding study as compared to the two diets with each component alone. Additional studies on the content of insulin, glucose, cholesterol, and triglycerides in serum and associated biomarkers seem to indicate that in addition to weight control, the binary combination diet has the added potential to treat/prevent diabetes and heart disease and to also concurrently prevent liver damage caused by the high-fat diet. There is, therefore, a need for future clinical studies to investigate if dietary bioprocessed black rice bran in combination with green tea extract can ameliorate and/or prevent human obesity, diabetes, and heart disease.

Technical Abstract: Black rice bran, a waste product produced during the commercial milling process of black rice which removes the bran and germ and leaves the starchy endosperm, contains bioactive anthocyanin, phenolic, and phytosteroid compounds that may be responsible for health effects. This study determined the effect of orally fed mice on a high-fat diet a polysaccharide-rich bioprocessed (fermented) black rice bran and a green tea extract individually and in combination on weight loss and on concurrent changes in blood content of glucose and insulin as well as in cholesterol, triglyceride, and high-density and low-density lipoproteins (HDL and LDL). At the end of the eight-week feeding study the combination diet caused a 56% weight loss compared to mice on a high-fat diet alone, whereas weight reduction caused by bioprocessed black rice bran and green tea extract individually were much lower. The weight loss caused by the combination diet seems to be the result of decreased dietary efficiency. The observed trends in the glucose and insulin data suggest that the combined diet also has anti-diabetes properties and the corresponding trends in the levels of the serum lipoproteins suggests that the combined diet might also protect against heart disease. Related studies of the dietary effects on the content, structure, and function of white adipose and liver tissues as well as obesity-related biomarkers (changes in the content of serum adiponectin and leptin hormones, insulin, and inflammatory cytokines) that are involved in the mechanism of fat-induced obesity and its prevention support the trends in the weight loss data. Based on the observed beneficial effects in both LT3-L1 preadipocyte cells and in the mice, we suggest the need for clinicians to investigate if the new multifunctional combination food product can also protect against chronic human diseases. Mechanistic aspects that govern the anti-obesity effects and suggestion for future research are discussed.