Location: Animal Disease Research
Title: Upper respiratory tract detection of mycoplasma ovipneumoniae employing nasopharyngeal SwabsAuthor
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Submitted to: BMC Veterinary Research
Publication Type: Peer Reviewed Journal Publication Acceptance Date: 10/21/2024 Publication Date: 11/1/2024 Citation: Herndon, D.R., Grossman, P.C., Hwang, J.K., Piel, L.M. 2024. Upper respiratory tract detection of mycoplasma ovipneumoniae employing nasopharyngeal Swabs. BMC Veterinary Research. 20:502. https://doi.org/10.1186/s12917-024-04342-y. DOI: https://doi.org/10.1186/s12917-024-04342-y Interpretive Summary: Flock-level prevalence of Mycoplasma ovipneumoniae is determined almost exclusively using nasal swabbing followed by molecular detection. Observations in our laboratory suggested that swabbing of the nasopharyngeal space may result in an improved diagnostic tool for the detection of this bacterium. To investigate this further, a domestic sheep flock was tested using both methods and the results were compared. Results demonstrate that use of nasopharyngeal swabbing improves diagnostic test efficiency and suggests that updated field-testing strategies should be considered for wild and domestic species when testing for M. ovipneumoniae. Technical Abstract: Flock-level prevalence of Mycoplasma ovipneumoniae is determined almost exclusively using nasal swabbing followed by molecular detection. Observations in our laboratory revealed that nasopharyngeal swabs resulted in higher sensitivity, reduced quantitative PCR inhibition, and generally higher genomic equivalents of the bacterium. To investigate these observations, a domestic sheep flock was swabbed using both nasal and nasopharyngeal techniques. Results demonstrate overall agreement of the increased diagnostic test efficiency when employing nasopharyngeal swabs. Updated field-testing strategies should be considered for wild and domestic species when testing for M. ovipneumoniae. |
