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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Cereal Crops Improvement Research » Research » Publications at this Location » Publication #415389
Research Project: Improvement of Disease and Pest Resistance in Barley, Durum, Oat, and Wheat Using Genetics and Genomics

Location: Cereal Crops Improvement Research

Title: Fine mapping of a QTL conferring susceptibility to spot blotch in barley

Author
item AMUSAN, OLAWUMI - North Dakota State University
item LENG, YUEQIANG - North Dakota State University
item Yang, Shengming
item ZHONG, SHAOBIN - North Dakota State University

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 6/1/2024
Publication Date: 6/10/2024
Citation: Amusan, O., Leng, Y., Yang, S., Zhong, S. 2024. Fine mapping of a QTL conferring susceptibility to spot blotch in barley. Meeting Abstract. 2024 Annual Meeting of the North Central Division of the American Phytopathological Society.

Interpretive Summary:

Technical Abstract: Spot blotch, caused by Bipolaris sorokiniana, is an important disease of barley. Use of host resistance is the most effective way to manage this disease. Our previous study identified two quantitative trait loci (QTL) (QSbs-1H-P1 and QSbs-7H-P1) conditioning susceptibility to spot botch caused by isolate ND85F in barley line ND5883 using a recombinant inbred line (RIL) population derived from the cross between Bowman (resistant) and ND5883 (susceptible). In this study, we aimed to finely map QSbs-1H-P1 flanked by two SNP markers spanning an ~460 Mb region on chromosome 1H. A cross was made between Bowman and a susceptible RIL carrying only QSbs-1H-P1 derived from the Bowman/ND5883 cross, and the F2 population was evaluated for infection responses to isolate ND85F. A total of 826 resistant F2 plants were identified and genotyped with peak and flanking markers of QSbs-1H-P1 through the Kompetitive Allele-Specific PCR (KASP) assay. Additional SNP markers were developed based on genome sequences of Bowman and ND5883, thereby delimiting the QTL between two markers 1H_ 23462390 and 1H_ 63553355 covering a physical distance of ~40Mb. More SNP markers will be developed for fine mapping of QSbs-1H-P1 with the goal of identifying the candidate gene for the QTL.