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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Virus and Prion Research » Research » Publications at this Location » Publication #419528
Research Project: Virulence Mechanisms, Microbiome Changes and Control Strategies for Priority Bacterial Infections in Swine

Location: Virus and Prion Research

Title: Comparative analysis of antimicrobial resistance and genetic diversity of Bordetella bronchiseptica swine isolates from the United States

Author
item Nicholson, Tracy
item Shore, Sarah

Submitted to: Frontiers in Microbiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/4/2024
Publication Date: N/A
Citation: N/A

Interpretive Summary: Bordetella bronchiseptica is bacterial pathogen that is pervasive in swine populations and serves multiple roles in respiratory disease. The goals of this study were to use whole-genome sequencing analysis to assess the genomic diversity and evaluate any potential antimicrobial resistance harbored by B. bronchiseptica isolates obtained from swine within the U.S. While a generally high degree of genomic conservation was observed among the swine B. bronchiseptica isolates, genetic diversity was identified within genes encoding adhesin factors and among a specific lineage of isolates. B. bronchiseptica isolates harbored a low number of antimicrobial resistance genes and exhibited phenotypic resistance to a low number of antibiotic classes. Combined the data suggests that B. bronchiseptica isolates are not serving as a source of antimicrobial resistance and mobile genetic elements in the swine production environment. This study serves as a baseline determining any potential risks associated with occupational exposure to these bacteria, while also providing data needed to address public health concerns. This information is important to public health professionals, scientists, and veterinarians.

Technical Abstract: Bordetella bronchiseptica is bacterial pathogen that is pervasive in swine populations and serves multiple roles in respiratory disease. The goals of this study were to use whole-genome sequencing (WGS) analysis to assess the sequence type (ST), genetic diversity of genes predicted to encode regulatory and virulence factors, and evaluate any potential antimicrobial resistance harbored by B. bronchiseptica isolates obtained from swine within the U.S. While a generally high degree of genomic conservation was observed among the swine B. bronchiseptica isolates, genetic diversity was identified within the fimNX locus and among the sequence type six (ST6) isolates. B. bronchiseptica isolates harbored a low number of antimicrobial resistance genes and exhibited phenotypic resistance to a low number of antibiotic classes. Combined the data suggests that B. bronchiseptica isolates are not serving as a source of antimicrobial resistance and mobile genetic elements in the swine production environment.