Location: Foreign Disease-Weed Science Research
Title: A 2023-2024 literature review of validation standards and diagnostic accuracyAuthor
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NASCIMENTO, DANIELE - Oklahoma State University |
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SHARMA, POONAM - Oklahoma State University |
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Luster, Douglas |
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CARDWELL, KITTY - Oklahoma State University |
Submitted to: PhytoFrontiers
Publication Type: Literature Review Publication Acceptance Date: 12/21/2024 Publication Date: N/A Citation: N/A Interpretive Summary: Not required for Literature Review (per ARS P&P 152.1v7) Technical Abstract: This review evaluates the progress in validating plant pathogen diagnostic assays by comparing recent literature (2023-2024) with previous assessments. Diagnostic accuracy relies on metrics like analytical and diagnostic specificity, sensitivity, repeatability, and reproducibility, essential for robust diagnostics and effective disease management. Our analysis of recent publications shows increased emphasis on analytical specificity and sensitivity, reflecting improvements in editorial standards, although consistent application of comprehensive validation standards remains limited. Technologies such as real-time PCR and loop-mediated isothermal amplification continue to be prominent, while emerging methods like Recombinase Polymerase Amplification combined with with gene editing reporter reagents show potential for rapid, sensitive detection. The findings underscore the need for more uniform validation practices, statistical rigor, and inclusion of all metrics to enhance reliability, reproducibility, and field utility of diagnostic tools. This continued focus is crucial for advancing plant health management and meeting diverse diagnostic demands. |