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1 - Germination Test Methods
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Click here for information on the seed testing papers we use.
Contact Curator Jeffrey Carstens.
Actaea
Malope 493959 Agastache 493218 Malva 490498 Alcea 493226 Matricaria 490482 Althea 493226 Monarda 493229 Anthemis 493794 Origanum 490496 Calendula 492151 Physocarpus 491385 Centranthus 493795 Potentilla 493227 Comarum 493796 Prunella
Dasiphora 493228 Rumex 493794 Dracocephalum 490482 Sanvitalia
Duchesnea 493794 Scrophularia
Echinacea 491056 Sibiraea
Glebionis 493228 Simsia
Heteranthemis 493957 Sorbaria 490493 Heteropappus 493793 Spergula 490499 Hyoscymous 491385 Sphaeralcea 493794 Hypericum 493229 Spiraea 490493 Hyssopus 493228 Tanacetum 492545 Lappula 493958 Vaccaria 490497 Lythrum 493794 Lavatera 490498
ENO: 493243 Actaea
Duration
Temperature
Fluorescent Lights
12 hours 20 ?C
ON
12 hours 8 ?C
OFF
Seeds are soaked in 1 mM GA3 overnight. Rinse seeds with tap water. Seeds are placed on top of 3 blotter papers (1 blue, 2 white - in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Transfer to germinator with temperatures alternating between 20 ?C with light for 12 hours and 8 ?C in darkness for 12 hours per 24 hour cycle. Leave at this regimen for many days. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts as needed.
ENO: 493218 Agastache
Duration
Temperature
Fluorescent Lights
12 hours
25 ?C
ON
12 hours
15 ?C
OFF
Seeds are placed on 3 blotter papers (2 blue, 1 white - in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Transfer to germinator alternating between 25 ?C with light for 12 hours and 15 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 8 and 16 days after start of test.
ENO: 493226 Alcea, Althea
Duration
Temperature
Fluorescent Lights
12 hours
20 ?C
ON
12 hours
20?C
OFF
Seeds are placed between blotter papers moistened with tap water in plastic boxes (5" x 5-1/4") with the following arrangement:
(top) 1 blue paper
seeds
1 white paper
(bottom) 1 blue paperLet sit overnight at room temperature. Transfer to germinator set at constant 20 ?C with light for 12 hours and dark for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts on 5 and 18 days after start of test. Chip remaining seed. Record final count after 5 more days.
ENO: 492151 Calendula
Duration
Temperature
Fluorescent Lights
12 hours
25 ?C
ON
12 hours
15?C
OFF
Seeds are placed on top of 2 blue blotter papers moistened with 0.1% KNO3 in plastic boxes (5" x 5-1/4"); substrate should be on drier side. Let sit overnight at room temperature. Transfer to germinator with temperatures alternatingalternating between 25 ?C with light for 12 hours and 15 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size:4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of test.
ENO: 493795 Centranthus
Duration
Temperature
Fluorescent Lights
12 hours
25 ?C
ON
12 hours
15?C
OFF
Seeds are placed on 3 blotter papers (1 blue, 2 white - in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4"). Let seeds imbibe overnight at room temperature. Transfer to germinator with temperatures alternating alternating between 25 ?C with light for 12 hours and 15 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur on 7, 14, and 21 days after start of test.
ENO: 493796 Comarum
Duration
Temperature
Fluorescent Lights
21 days
4 ?C
---
12 hours
25 ?C
ON
12 hours
15 ?C
OFF
Seeds are placed on top of 3 blue blotter (1 blue, 2 white - in contact with seeds) papers moistened with 0.1% KNO3 solution in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Prechill at 4 ?C for 21 days. Transfer to germinator with temperatures alternating alternating between 25 ?C with light for 12 hours and 15 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur on 7, 14, and 21 days after start of test.
ENO: 493228 Dasiphora, Glebionis, Hyssopus
Duration
Temperature
Fluorescent Lights
7 days
4 ?C
---
12 hours
25 ?C
ON
12 hours
15 ?C
OFF
Seeds are placed on top of 2 blue blotter papers moistened with 0.1% KNO3 solution in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Prechill at 4 ?C for 7 days. Transfer to germinator with temperatures alternating alternating between 25 ?C with light for 12 hours and 15 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur on 7, 14, and 21 days after start of germination test.
ENO: 490482 Dracocephalum, Matricaria
Duration
Temperature
Fluorescent Lights
12 hours
20 ?C
ON
12 hours
20?C
OFF
Seeds are placed on top of 3 blotter papers (1 blue, 1 white, 1 blue - in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Transfer to germinator set at constant 20 ?C with light for 12 hours and dark for 12 hours per 24 hour cycle. Replication and sample size:4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of test.
ENO: 493794 Anthemis, Duchesnea,
Lythrum, Rumex, SphaeralceaDuration
Temperature
Fluorescent Lights
12 hours
30 ?C
ON
12 hours
20 ?C
OFF
Seeds are placed on top of three blotter papers (1 blue, 2 white - in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4"). Let seeds imbibe overnight at room temperature. Transfer to germinator with temperatures alternating alternating between 30 ?C with light for 12 hours and 20 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of test.
ENO: 491056 Echinacea
Duration
Temperature
Fluorescent Lights
28 days
4?C
---
12 hours
25 ?C
ON
12 hours
25?C
OFF
Soak seeds ovenight in 1 mM Ethephon. Transfer to germination boxes: seeds are placed on top of 2 blue blotter papers moistened with tap water. Prechill at 4 ?C for 28 days. Transfer to germinator with temperature set to a constant 25 ?C with light for 12 hours and dark for 12 hours per 24 hour cycle. Replication and sample size:4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germination test.
ENO: 493957 Heteranthemis
Duration
Temperature
Fluorescent Lights
12 hours
15 ?C
ON
12 hours
15 ?C
OFF
Seeds are placed on top of 3 blotter papers (1 blue, 1 white, 1 blue - in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Transfer to germinator set at constant 20 ?C with light for 12 hours and dark for 12 hours per 24 hour cycle.Replication and sample size:4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of test.
ENO: 493793 Heteropappus
Duration
Temperature
Fluorescent Lights
28 days
4 ?C
---
12 hours
25 ?C
ON
12 hours
15 ?C
OFF
Seeds are placed on top of 3 blotter papers (1 blue, 2 white - in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Prechill at 4 ?C for 28 days. Transfer to germinator with temperatures alternating alternating between 25 ?C with light for 12 hours and 15 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germination test.
ENO: 491385 Hyoscymous, Physocarpus
Duration
Temperature
Fluorescent Lights
21 days
4 ?C
---
12 hours
25 ?C
ON
12 hours
15 ?C
OFF
Seeds are placed between blotter papers moistened with 0.1% KNO3 in plastic boxes (5" x 5-1/4") with the following arrangement:
(top) 1 blue paper
seeds
1 white paper
(bottom) 1 blue paperLet sit overnight at room temperature. Prechill for 21 days. Remove top blotter paper and transfer to germinator with temperatures alternatingalternating between 25 ?C with light for 12 hours and 15 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germination test.
ENO: 493229 Hypericum, Monarda
Duration
Temperature
Fluorescent Lights
12 hours
25 ?C
ON
12 hours
15 ?C
OFF
Seeds are placed on top of 3 blotter papers (1 blue, 1 white, 1 blue - in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Transfer to germinator with temperatures alternating alternating between 30 ?C with light for 12 hours and 20 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size:4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of test.
ENO: 493958 Lappula
Duration
Temperature
Fluorescent Lights
14 days
4?C
---
12 hours
15 ?C
ON
12 hours
15?C
OFF
Seeds are placed on top of 3 blotter papers (1 blue, 1 white, 1 blue - in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Prechill at 4 ?C for 14 days. Transfer to germinator with temperature set to a constant 15 ?C with light for 12 hours and dark for 12 hours per 24 hour cycle. Replication and sample size:4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germination test.
ENO: 490498 Lavatera, Malva
Duration
Temperature
Fluorescent Lights
Constant
Room Temp
DARK
Seeds are placed between 2 moist blotter papers and allowed to imbibe water overnight. Seeds are then chipped and placed between blotter papers moistened with tap water in the following arrangement:
(top) 1 blue paper
seeds
1 white paper
(bottom) 1 blue paperThese are kept at room temperature in the dark. Replication and sample size: 4 reps of 50 sd for total of 200 seeds.First count occurs in 4 to 8 days after chipping seed. Germination counts occur weekly after that.
ENO: 493959 Malope
Duration
Temperature
Fluorescent Lights
12 hours
15 ?C
ON
12 hours
15?C
OFF
Seeds are placed between blotter papers moistened with tap water in plastic boxes (5" x 5-1/4") with the following arrangement:
(top) 1 blue paper
seeds
1 white paper
(bottom) 1 blue paperLet sit overnight at room temperature. Transfer to germinator set at constant 15 ?C with light for 12 hours and dark for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germination test.
ENO: 490496 Origanum
Duration
Temperature
Fluorescent Lights
Constant
15 ?C
---
Seeds are placed between blotter papers moistened with tap water in plastic boxes (5" x 5-1/4") with the following arrangement:
(top) 1 blue paper
seeds
1 white paper
(bottom) 1 blue paperLet sit overnight at room temperature. Transfer to germinator with temperature set to a constant 15 ?C. Replication and sample size:4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germination test.
ENO: 493227 Potentilla
Duration
Temperature
Fluorescent Lights
21 days
4 ?C
---
7 days
10 ?C
OFF
12 hours
20 ?C
ON
12 hours
20?C
OFF
Seeds are placed on top of 3 blotter papers (1 blue, 1 white, 1 blue - in contact with seeds) moistened with 0.1% KNO3 in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Prechill at 4 ?C for 21 days. Transfer to germinator with temperature set to a constant 10 ?C and darkness for 7 days. Then transfer to germinator with temperature set for 20 C with light for 12 hours and darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after transfer to 20 ?C germinator.
ENO: 493225 Prunella
Duration
Temperature
Fluorescent Lights
12 hours
30 ?C
OFF
12 hours
20 ?C
OFF
Seeds are placed between blotter papers moistened with tap water in the following arrangement:
(top) 1 blue paper
seeds
1 white paper
(bottom) 1 blue paperLet sit overnight. Transfer to germinator with temperatures alternating between 30 ?C for 12 hours and 20 ?C for 12 hours per 24 hour cycle, no lights. Replication and sample size: 4 reps of 50 sd for total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germination test.
ENO: 490484 Sanvitalia
Duration
Temperature
Fluorescent Lights
7 days
4 ?C
---
12 hours
25 ?C
ON
12 hours
15 ?C
OFF
Seeds are placed on top of 2 blue blotter papers moistened with tap water in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Prechill at 4 ?C for 7 days. Transfer to germinator with temperatures alternating alternating between 25 ?C with light for 12 hours and 15 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur on 7, 14, and 21 days after start of germination test.
ENO: 493797 Scrophularia
Duration
Temperature
Fluorescent Lights
21 days
4 ?C
---
12 hours
30 ?C
ON
12 hours
20 ?C
OFF
Seeds are placed on top of 3 blotter papers (1 blue, 2 white - in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Prechill at 4 ?C for 21 days. Transfer to germinator with temperatures alternating alternating between 30 ?C with light for 12 hours and 20 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germination test.
ENO: 490493 Sibiraea, Sorbaria, Spiraea
Duration
Temperature
Fluorescent Lights
28 days
4 ?C
---
12 hours
30 ?C
ON
12 hours
20 ?C
OFF
Seeds are placed on top of 3 blotter papers (1 blue, 1 white, 1 blue - in contact with seeds) moistened with 0.1% KNO3 solution in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Prechill at 4 ?C for 28 days. Transfer to germinator with temperatures alternating alternating between 30 ?C with light for 12 hours and 20 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germination test.
ENO: 493960 Simsia
Duration
Temperature
Fluorescent Lights
7 days
4?C
---
12 hours
30 ?C
ON
12 hours
20?C
OFF
Seeds are placed in folded paper towels1 moistened with tap water and placed in plastic tubs. Let sit overnight at room temperature. Prechill at 4 ?C for 7 days. Transfer to germinator with temperatures alternatingalternating between 30 ?C with light for 12 hours and 20 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germination test.
1Seeds are wrapped in 2 sheets of germination toweling and replicated four times. The four replications are rubber banded together and eight accessions are placed in plastic tubs. Towels are kept moist with tap water during the germination period.
ENO: 490499 Spergula
Duration
Temperature
Fluorescent Lights
7 days
4?C
---
12 hours
15 ?C
ON
12 hours
15?C
OFF
Seeds are placed on top of 2 blue blotter papers moistened with tap water in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Prechill at 4 ?C for 7 days. Transfer to germinator with temperature set to a constant 15 ?C with light for 12 hours and dark for 12 hours per 24 hour cycle. Replication and sample size:4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germination test.
ENO: 492545 Tanacetum
Duration
Temperature
Fluorescent Lights
12 hours
25 ?C
ON
12 hours
15 ?C
OFF
Seeds are placed on top of 2 blue blotter papers moistened with 0.1% KNO3 solution in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Transfer to germinator with temperatures alternating alternating between 25 ?C with light for 12 hours and 15 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur on 7, 14, and 21 days after start of germination test.
ENO: 490497 Vaccaria
Duration
Temperature
Fluorescent Lights
7 days
4?C
---
12 hours
15 ?C
ON
12 hours
15?C
OFF
Seeds are placed on top of 2 blue blotter papers moistened with with 0.1% KNO3 solution in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Prechill at 4 ?C for 7 days. Transfer to germinator with temperature set to a constant 15 ?C with light for 12 hours and dark for 12 hours per 24 hour cycle. Replication and sample size:4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germination test.
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