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1 - Germination Test Methods
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Click here for information on the seed testing papers we use.
Contact Curator Kathy Reitsma.
Cichorium 491000 Cucurbita 490384 Cucumis 490382 Daucus 493996 Ocimum 490973 Pastinaca 490972
ENO: 491000 Cichorium
Duration
Temperature
Fluorescent Lights
12 hours
30 ?C
ON
12 hours
20 ?C
OFF
Seeds are placed on top of two blue blotter papers moistened with 0.1% KNO3 in plastic boxes (5" x 5-1/4"). Let seeds imbibe overnight at room temperature. Transfer to germinator with temperatures alternating alternating between 30 ?C with light for 12 hours and 20 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 5 and 14 days after start of test.
ENO: 490384 Cucurbita
Duration
Temperature
Fluorescent Lights
30 ?C
OFF
20 ?C
OFF
Seeds are placed in folded paper towels moistened with tap water (substrate on dry side) and placed in plastic tubs. These are enclosed by a black plastic bag to exclude light. Transfer to germinator with temperatures alternating between 30 ?C for 12 hours and 20 ?C for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Counts are done 4 and 8 days after start of test.
ENO: 490382 Cucumis
Duration
Temperature
Fluorescent Lights
30 ?C
OFF
20 ?C
OFF
Seeds are placed in folded paper towels moistened with tap water (substrate on dry side) and placed in plastic tubs. These are enclosed by a black plastic bag to exclude light. Transfer to germinator with temperatures alternating between 30 ?C for 12 hours and 20 ?C for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Counts are done 4 and 8 days after start of test.
ENO: 493996 Daucus
Duration
Temperature
Fluorescent Lights
25 ?C
ON
15 ?C
OFF
Seeds are placed on top of 2 blue blotter papers moistened with tap water in plastic boxes (5" x 5-1/4"). Let sit overnight at room temperature. Transfer to germinator with temperatures alternating between 25 ?C with light for 12 hours and 15 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Counts are done 7, 14, 21, and (if needed) 28 days after start of test.
ENO: 490973 Ocimum
Duration
Temperature
Fluorescent Lights
12 hours
30 ?C
ON
12 hours
20?C
OFF
Seeds are placed on top of 2 blue blotter papers moistened with 0.1% KNO3 in plastic boxes (5" x 5-1/4"). Let sit overnight at room temperature. Transfer to germinator with temperatures alternatingalternating between 30 ?C with light for 12 hours and 20 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size:4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7 and 14 after start of test.
ENO: 490972 Pastinaca
Duration
Temperature
Fluorescent Lights
25 ?C
ON
15 ?C
OFF
Seeds are placed in folded paper towels moistened with tap water and placed in plastic tubs. Let sit overnight at room temperature. Transfer to germinator with temperatures alternating between 25 ?C with light for 12 hours and 15 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Counts are done at 7, 14, and 21 days after start of test.
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