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Simon: Pubs: 99plan0032
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Plant Cell Reports (1999) 19: 32-36

J. M. Myers - P. W. Simon

Regeneration of garlic callus as affected by clonal variation, plant growth regulators and culture conditions over time

Received: 24 September 1998 / Revision received: 27 January 1999 / Accepted: 26 February 1999

Abstract  A long-term regeneration system for garlic (Allium sativum L.) clones of diverse origin was developed. Callus was initiated on a modified Gamborg's B-5 medium supplemented with 4.5 ?M 2,4-D and maintained on the same basal medium with 4.7 ?M picloram+0.49 ?M 2iP. Regeneration potential of callus after 5, 12 and 16 months on maintenance medium was measured using several plant growth regulator treatments. The 1.4 ?M picloram+13.3 ?M BA treatment stimulated the highest rate of shoot production. Regeneration rate decreased as callus age increased, but healthy plantlets from callus cultures up to 16-months-old were produced for all clones. Regeneration of long-term garlic callus cultures could be useful for clonal propagation and transformation.

Key words  Allium sativum •• Benzyladenine •• Picloram •• Thidiazuron •• 2,4-D •• 2iP

Communicated by C.F. Quiros

J.M. Myers •• P. W. Simon (envelope)
USDA-ARS Vegetable Crops Research, Department of
Horticulture, 1575 Linden Drive, University of
Wisconsin-Madison, Madison, WI 53706, USA
e-mail: psimon@facstaff.wisc.edu
Fax: +608-262-4743