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1 - Germination Test Methods
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Evaluation Numbers - infrequent or no longer used:
(General, Historic, Special Care)
Historic
ENO: 11
Historic
Germinations are performed in a high humidity germinator set at 12 hours 20 degrees centigrade in the dark alternating with 12 hours 30 degrees centigrade in the light. 50 seeds are wrapped in 2 paper towels for each replication. Replications are rubber banded together. 10-20 accessions are placed in plastic tubs with holes in the bottom. Towels are kept moist during germination period. Evaluations are performed at 7 and 10 days.
Special Care
ENO: 24
Special Care
Seeds are rinsed in 10% bleach and placed on blotters in deep plastic boxes. Separation of seed is 2 cm or more. Fungicides are applied when needed. Boxes are placed in germinator set on alternating 20-30 degrees C and alternating light-dark. When seedling leaves begin emerging from the coleoptile, they are transplanted to 7.6 cm jiffy pots. Soil is added to the pots as the seedlings develop. These jiffy pots are transplanted to large plastic pots when the seedlings are fully established.
General
ENO: 150
General
Seeds are germinated on 2 blotter papers moistened with a 0.1% KN03 solution in plastic boxes (5" x 5-1/4"). Temperatures alternate at 30C with light for 12 hours and 20C in darkness for 12 hours per 24 hour cycle.
Historic
ENO: 180
Historic
Germinations are performed at room temperature (~20 degrees C) with 0.2% percent potassium nitrate as the medium. Fifty seeds are put on top of blotters for each replication. The number of germinated, abnormal, and hard seeds are counted at 3 and 7 days after placing on blotters.
General
ENO: 367
General
Seeds are germinated on blotters moistened with 0.2% KNO3 water in plastic boxes. The temperature is 10 deg C, the daylength is 12 to 14 hours, for 21 days.
Special Care
ENO: 399
Special Care
Seeds are soaked in water overnight or a minimum of four hours. Seed coats are removed. Seeds are placed on two blotters in a germ box. Keep blotters soaked with excess water at all times. Place under 12 hours of light at room temperature. Count germs every seven days for 21 days.
Historic
ENO: 400
Historic
Seeds are placed on two blotter pads in a germ box. Blotter pads are kept soaked with excess water at all times. Germ box is placed in 12 hours of light at room temperature. Germs are counted every seven days for 21 days.
Historic
ENO: 404
Historic
Four replicates of fifty seeds are each folded into a double layer of germ toweling moistened with water and placed in a lighted germination chamber ( 16/8 ) at 20 degrees C. Evaluations are performed at 4 and 7 days following I.S.T.A. and A.O.S.A. rules. An optional prechill at 5-10 degrees C for up to 7 days may be used to hasten and unify germination and may be used as test environment.
Additional comments: Crambe: 0.1% potassium nitrate solution is used to initially moisten toweling.
Historic
ENO: 490012
Historic
Performed by ISU Seed Lab. Stress condition.
Historic
ENO: 490013
Historic
Performed by ISU Seed Lab.
Historic
ENO: 490383
Historic
Seeds are placed on top of 2 blue blotter papers and one sheet of pleated paper moistened with tap water (substrate on dry side) in plastic boxes (5" x 5-1/4"). Let sit overnight at room temperature. Transfer to germinator with temperatures alternating between 30 ?C with light for 12 hours and 20 ?C in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Counts are done 7, 21, and 28 days after start of test.
Historic
ENO: 490483
Historic
No method described except in evaluation name.
Historic
ENO: 490487
Historic
No method described except in evaluation name.
General
ENO: 490500
General
Seeds are imbibed with tap water on 2 germination papers in a plastic box (5" x 5-1/4"). Temperature and light regimen is 20 C with fluorescent light per 12 hours per 24 hour cycle, alternating with 20 C in darkness.
Historic
ENO: 490501
Historic
No method described except in evaluation name.
Historic
ENO: 490502
Historic
No method described except in evaluation name.
Historic
ENO: 490506
Historic
No method described except in evaluation name.
Special Care
ENO: 490639
Special Care
Seeds are prehumidified 24 hours by placing seeds in accelerated aging tray with seeds above distilled water. Temperature is held at a constant 22C. Lights are on 0800-2200 and off 2200-0800. Seeds are then transferred to a double layer of germination blotter paper with 20 ml of distilled water. From 0800-2200 temperature is 22C and lights are on. From 2200-0800 temperature is 20C and lights are off. Counts are made at 3 day intervals until completion. Note: Accelerated aging trays are transparent polystyrene containers (4 5/16" x 4 5/16" x 1 3/8") that have a wire mesh screen that keeps seeds approximately 5/8" above the bottom of the container. The bottom of the container has a small amount of water added to achieve the high humidity atmosphere.
Special Care
ENO: 490674
Special Care
This method is the same as NC7.GERMS.MAIZE.STANDARD except the quantity and replications are different because there is a low quantity of seed to germinate. The recommended quantity for this method is 4 replications of 10 seeds each. Germinations are performed in a high humidity germinator set at 12 hours 20 degrees centigrade in the dark alternating with 12 hours 30 degrees centigrade in the light. Seeds are wrapped in 2 paper towels for each replication. Replications are rubber banded together. Towels are kept moist during germination period.
Historic
ENO: 490995
Historic
4 Reps 50 ct ea.; Towels, 8/16 hours lights at 20/30 C.
Asparagus is now curated at NE9, Geneva, NY.
Special Care
ENO: 491028
Special Care
Seeds soaked in a .25% Thiram solution for 10-12 hours, drained and stored in the dark at 35-45 F (~4 C) for 6-8 weeks. They are then placed in a 25/23 germinator with lights 12/12 on/off.
General
ENO: 491156
General
Seeds are imbibed with tap water on 2 germination papers in a plastic box (5" x 5-1/4"). Temperature is held constant at 25 C with 12 hours of fluorescent light per 24 hour cycle.
Historic
ENO: 491200
Historic
No method described except in evaluation name.
Historic
ENO: 491297
Historic
Germination data received from Fargo, ND when crop was transferred. Germination percentage was determined from 100 seeds placed in a 9 cm diameter petri dish on a filter paper wetted with distilled water. The number of germinated seeds were recorded after 72 hours.
General
ENO: 491369
General
Germinations were conducted utilizing 5" x 5 1/4" x 1 1/4" germination boxes with 2 germination papers in each box. Germ papers were initially wetted with 0.1% KNO3 distilled water solution and the seeds were then evenly distributed on the germ paper. Germ boxes were then placed into germination chambers set at 30 C with fluorescent lights for 14 hrs alternating with 20 C and dark for 10 hrs. Follow up wettings of the germination papers were with distilled water. [Note: 0.1% KNO3 was used to alleviate negative effect of higher concentrations sometimes prescribed. 14 hr / 10 hr cycles were used to more closely simulate temperate zone conditions.]
Historic
ENO: 491386
Historic
No method described except in evaluation name.
Historic
ENO: 491460
Historic
Two replications of 100 seeds each; Towels; Pre-chill ( 4C ) for 7 days; 20/30 dark; 7 and 14 days. Then, light ( 16/8h ); and counts at 21 and 28 days. May continue to germinate up to 6 weeks.
Occasionally used
ENO: 491489
Occasionally used
Towels; H20; Pre-chill (7-14d@4C); 10/30C; 7,14 and 21days.
General
ENO: 491579
General
Seeds are imbibed with tap water on 2 germination papers in a plastic box (5" x 5-1/4"). Temperature and light regimen is 25 C with fluorescent light per 12 hours per 24 hour cycle, alternating with 15 C in darkness.
General
ENO: 491609
General
Two replications of 100 seeds each; Blotter (TP) moist with 0.1%KNO3; keep in cooler 5 deg. centigrade, counts 7, 14, 21 and 28 days.
Historic
ENO: 491624
Historic
Towels; 0.1% KNO3; 16/8 light; 20/30C; 7 and 14 days. If no response after 10 days high temp. is moved up to 32C. *With proper alternating temperature regimen light is not an absolute requirement. (Method from previous technician; moved from standard method, which was set back to original standard eval description.)
Historic
ENO: 491849
Historic
No method described except in evaluation name.
General
ENO: 491857
General
Germinations are performed at room tempurature close to 25 C. 50 seeds are wrapped in 2 paper towels for each replication. Replications are rubber banded together. 10-20 accessions are placed in plastic tubs without holes in the bottom. Towels are kept moist during germination period. Evaluations are performed at 7 and 10 days.
Historic
ENO: 491878
Historic
Two replications of 100 seeds each, or 4 replications of 50 seeds each; Blotter (TP); Pre-chill (4C) 7 days; 20/30 dark with counts 7 and 14 days; then 16/8hrs lights, 20/30 C with counts 7, 14, 21 and 28 days. Additional comments: The initial dark environment can be ignored for those species with a known light requirement; which are numerous in this family. Chaerophyllum and Eryngium can take up to two months to fully germinate (Chaerophyllum will not usually germinate until at least 4 weeks). (Method from previous technician; moved from standard method, which was set back to original standard eval description.) Use only tap water (changed from KNO3 in 2002) add tap water when dry. Use the 20/30 germinator with light/dark in most cases. In cases where two seeds are attached together, esp. in Coriandrum, the seeds should be separated, seeds that crumble (because they are undeveloped) are not counted and are excluded from the germination sample of 200 non-crumbling seeds.
Historic
ENO: 492108
Historic
BLOTTERS, KNO3 AT 20C. COUNTS ON DAYS 4 AND 7.
Historic
ENO: 492293
Historic
4 Reps of 50 seed/rep. Total of 200 seed. 2 sheets of paper toweling wetted with water. Constant 15C germinator with 12h light/12h dark. Counts on day 6 and 21.
Occasionally used
ENO: 492308
Occasionally used
BLOTTER BOXES WITH WATER. EXCESSIVE MOISTURE(VERY WET). 20C CONSTANT WITH 12hr DARK/12hr LIGHT. 1ST COUNT DAY 7 2ND AND FINAL COUNT DAY 14.
Historic
ENO: 492423
Historic
TAP WATER, BLOTTER BOXES, 4 REPS, 50 SEEDS/REP. 20C CONSTANT WITH 12d/12L
Special Care
ENO: 492426
Special Care
0.1% KNO3 2 BLOTTERS IN BLOTTER BOXES. 200 SEED, 50 SEED/REP. SCARIFY SEED WITH 150 GRIT SANDPAPER ON RUBBING BOARD. 20C CONSTANT 24H DARK.
Historic
ENO: 492450
Historic
Blotter boxes with water. Germinator is set at 15/25 12 hours dark/12 hours light. Counts on day 7 and day 21.
Special Care
ENO: 492558
Special Care
200 SEED TOTAL. 4 REPS, 50 SEED/REP. ALL SEEDS ARE CHIPPED WITH A STRAIGHT EDGE RAZOR BLADE. SEEDS ARE THEN PLACED IN A BLOTTER BOX WITH 2 BLUE BLOTTERS AND ONE WHITE BLOTTER MOISTENED WITH WATER. BOXES WERE COVERED WITH A BLACK TRASH BAG AND PLACED IN A CABINET. COUNTS ON DAY 7,14 AND 21.
Historic
ENO: 492566
Historic
Use two full size germination papers for each germ box. Add 25ml of 3 mM GA3 solution (= 1.038 gm/l or approx. 1 gm GA3 / liter DI water). Put 50 wild flax seeds on germ paper. Put in germination chamber set for: Temperature: 23 C (12 hrs) / 9 C (12 hrs). That is, from 8:00 am to 8:00 pm. Lights: On (11.5 hrs) / Off (12.5 hrs). That is, from 8:30 am to 8:00 pm. GA3 treatment should last 48 hrs. Then, transfer seeds to new germ paper wetted with tap or DI water. Keep germ boxes in the 23/9 C regimen as described above for entire germ test. Recommend counts be taken at 10 da, 14 da, and 21 da.
General
ENO: 492572
General
Tetrazolium Test.
Special Care
ENO: 492614
Special Care
ALL SEED IS PLACED BETWEEN TWO MOISTENED BLOTTER PAPERS IN THE DARK, AT ROOM TEMP. FOR 2 TO 3 DAYS. SEEDS THAT DO NOT HAVE RADICLE PROTRUSION AFTER CONDITIONING TREATMENT ARE THEN EXCISED FROM THEIR PERICARP AND SEEDCOAT. SEEDS THAT HAVE RADICLE PROTRUSION ARE NOT EXCISED. ALL SEEDS ARE THEN PLACED ON BLOTTER PAPER AND PLACED IN A 20/30 GERMINATOR WITH 12d/12L UNTIL READY TO COUNT.
Special Care
ENO: 492640
Special Care
200 SEEDS. 4 REPS WITH 50 SEED/REP. BLOTTER BOXES WITH WATER. SET AT ROOM TEMPERATURE OVERNIGHT AND THEN PRECHILL FOR 3 MONTHS AT 4C. TRANSFER TO A 20/30 GERMINATOR WITH 12D/12L. COUNTS ON DAY 7, 14 AND 21.
General
ENO: 492969
General
Blotter Boxes. 2 blue blotters and 1 white. Tap water. Place in cold room for 90 days and then transfer to a 20/30 germinator with 12hr. dark/12hr light.
Historic
ENO: 493010
Historic
No method described except in evaluation name.
Historic
ENO: 493018
Historic
Four replicates of fifty seeds are each placed onto a double layer of blue germ paper and a single layer of white germ blotters, moistened with water and placed in a lighted germination chamber ( 12/12 ) at 20 degrees C. Evaluations are performed at 7 days only. An optional prechill at 5-10 degrees C for up to 7 days may be used to hasten and unify germination and may be used as test environment.
General
ENO: 493044
General
200 SEED, 4 REPS, 50SEED/REP. BLOTTER BOXES. 0.1% KNO3. 5 DAY PRECHILL. 15C CONSTANT. 12D/12L.
Historic
ENO: 493219
Historic
KNO3, TOP OF BLOTTERS LAYERED 1 BLUE, 1 WHITE, 1 BLUE. 20C/30C, 12D/12L. COUNTS DAY 7 + 28.
Historic
ENO: 493222
Historic
BLOTTER BOXES, INBETWEEN BLOTTERS, BLUE, WHITE, SEEDS, BLUE ON TOP. H20. 15C/30C COUNTS ON DAYS 4 AND 10.
Historic
ENO: 493375
Historic
PETRI PLATES, DISTILLED H20, CUT BLOTTERS INTO SMALL SQUARES PLACE 2 BLUE AND 1 WHITE. PLACE SEEDS ON TOP OF WHITE BLOTTER AND PLACE IN 8/20 CELCIUS GERMINATOR 12D/12L. CHECK FOR RADICLE EMERGENCE IN 7 DAYS.
Occasionally used
ENO: 493525
Occasionally used
BLOTTER BOXES. 1BLUE BLOTTER, 1WHITE, 1 BLUE ON TOP. VERY WET BLOTTERS. SEEDS ON TOP OF BLOTTERS. SPRAY LIGHTLY WITH MORE WATER AND SET AT ROOM TEMPERATURE OVERNIGHT. 2 WEEK PRECHILL. PLACE IN GERMINATOR 7C/15C, 12/12 LIGHTING.
Special Care
ENO: 493603
Special Care
Samples were x-rayed. Seeds that were filled are considered normal and empty seeds are considered abnormal.
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