| Simon: Pubs: 99tag_0058 |
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Theor Appl Genet (1999) 99: 58-64
B. S. Vivek . P. W. Simon
Linkage relationships among molecular markers and storage root traits of carrot (Daucus carota L. ssp. sativus)
Received: 2 September 1998/Accepted: 28 November 1998
Abstract A 109-point linkage map consisting of three phenotypic loci (P1, Y2, and Rs), six restriction fragment length polymorphisms (RFLPs), two random amplified polymorphic DNAs (RAPDs), 96 amplified fragment length polymorphisms (AFLPs), and two selective amplification of microsatellite polymorphic loci (SAMPL) was constructed for carrot (Daucus carota L. ssp. sativus; 2n=2x=18). The incidence of polymorphism was 36% for RFLP probes, 20% for RAPD primers, and 42% for AFLP primers. The overall incidence of disturbed segregation was 18%. Linkage relationships at a LOD score of 4.0 and 
=0.25 indicated 11 linkage groups. The total map length was 534.4 cM and the map was clearly unsaturated with markers spaced at 4.9 cM. AFLP P6B15 was 1.7 cM from P1, AFLP P1B34 was 2.2 cM from Y2, and AFLP P3B30XA was 8.1 cM from Rs.
Key words Genetic map RFLP . AFLP . RAPD . SAMPL .Daucus carota L. ssp. sativus
Communicated by G. Wenzel
B. S. Vivek1. P. W. Simon (
)
USDA-ARS, Vegetable Crops Research Unit,
Department of Horticulture, 1575 Linden Drive,
University of Wisconsin-Madison, Madison, WI 53705, USA
Fax: +1 608 262 4743
E-mail: psimon@facstaff.wisc.edu
Present address:
1CIMMYT, Lisboa 27, Apdo. Postal 6-641, Mexico D.F.,
06600, Mexico
