Huang Lab : USDA ARS

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Huang Lab

HUANG LAB

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- Liping Huang, Ph.D.
  
  Research Leader
  
  Adjunct Professor, UC Davis Department of Nutrition
  
  Office: (530) 754-5756
  
  Email address: liping.huang@usda.gov
  
  Research Interests
  
  The Huang lab is focused on the function of zinc transporters in maintaining body zinc homeostasis. Specifically 1) Determine novel functions of zinc related to energy metabolism, insulin resistance in skeletal muscle, and adipose tissue and immune function and 2) Measure and validate novel functional biomarkers of Zn status in response to supplementation of deficient human subjects.
  
  Huang Lab Themes
  
  Our research studies focus on cellular zinc regulation and its links to modulation of body weight gain, adiposity, glucose and insulin homeostasis, and immunity. The goal is to understand the mechanism of zinc action in the body.
  
  • QTL mapping of body weight and adiposity in mice
  
  • Zinc and immunity
  
  • Zinc and glucose/insulin metabolism

- ARS Employees
  
  Catherine Kirschke, Molecular Biologist
  
  Surapun Tepaamorndech, PhD candidate, Integrated Genoics and Genetics Graduate Group

- (Clicking on the reprint icon will take you to the publication reprint.)
- Association of lactase persistence genotypes (rs4988235) and ethnicity with dairy intake in a healthy U.S. population-(Peer Reviewed Journal)
  Chin, E.L., Huang, L., Bouzid, Y.Y., Kirschke, C.P., Durbin-Johnson, B., Baldiviez, L.M., Bonnel, E.L., Keim, N.L., Korf, I., Stephensen, C.B., Lemay, D.G. 2019. Association of lactase persistence genotypes (rs4988235) and ethnicity with dairy intake in a healthy U.S. population. Nutrients. https://doi.org/10.3390/nu11081860.
- Temporal changes in postprandial blood transcriptomes reveal subject-specific pattern of expression of innate immunity genes after a high-fat meal-(Peer Reviewed Journal)
  Lemay, D.G., Huang, S., Huang, L., Alkan, Z., Kirschke, C.P., Burnett, D.J., Wang, Y., Hwang, D.H. 2019. Temporal changes in postprandial blood transcriptomes reveal subject-specific pattern of expression of innate immunity genes after a high-fat meal. Journal of Nutritional Biochemistry. 72:108209. https://doi.org/10.1016/j.jnutbio.2019.06.007.
- Subcongenic analysis of a quantitative trait locus affecting body weight and glucose metabolism in zinc transporter 7 (znt7)-knockout mice-(Peer Reviewed Journal)
  Huang, L., Tepaamorndech, S., Kirschke, C.P., Cai, Y., Zhao, J., Cao, X., Rao, A. 2019. Subcongenic analysis of a quantitative trait locus affecting body weight and glucose metabolism in zinc transporter 7 (znt7)-knockout mice. BioMed Central (BMC) Genetics. 20:19. https://doi.org/10.1186/s12863-019-0715-2.
- SLC30A family expression in the pancreatic islets of humans and mice: cellular localization in the ß-cells-(Peer Reviewed Journal)
  Cai, Y., Kirschke, C.P., Huang, L. 2018. SLC30A family expression in the pancreatic islets of humans and mice: cellular localization in the ß-cells. Journal of Molecular Histology. 49(2):133-145. https://doi.org/10.1007/s10735-017-9753-0.
- Design and implementation of a cross-sectional nutritional phenotyping study in healthy US adults-(Peer Reviewed Journal)
  Baldiviez, L.M., Keim, N.L., Laugero, K.D., Hwang, D.H., Huang, L., Woodhouse, L.R., Burnett, D.J., Zerofsky, M., Bonnel, E., Allen, L.H., Newman, J.W., Stephensen, C.B. 2017. Design and implementation of a cross-sectional nutritional phenotyping study in healthy US adults. Biomed Central (BMC) Nutrition. 3(79):1-13. https://doi.org/10.1186/s40795-017-0197-4.
- Aberrant fatty acid metabolism in the skeletal muscle contributes to insulin resistance in zinc transporter 7 (Znt7) knockout mice-(Peer Reviewed Journal)
  Huang, L., Tepaamorndech, S., Kirschke, C.P., Newman, J.W., Keyes, W.R., Pedersen, T.L. 2018. Aberrant fatty acid metabolism in the skeletal muscle contributes to insulin resistance in zinc transporter 7 (Znt7) knockout mice. Journal of Biological Chemistry. 293(20):7549-7563. doi:10.1074/jbc.M117.817692.
- ZNT7 binds to CD40 and influences CD154-triggered p38 MAPK activity in B lymphocytes-a possible regulatory mechanism for zinc in immune function-(Peer Reviewed Journal)
  Tepaamorndech, S., Oort, P.J., Kirschke, C.P., Yimeng, C., Huang, L. 2017. ZNT7 binds to CD40 and influences CD154-triggered p38 MAPK activity in B lymphocytes-a possible regulatory mechanism for zinc in immune function. FEBS Open Bio. 7(5):675–690. doi: 10.1002/2211-5463.12211.
- Down-regulation of zinc transporter 8 (SLC30A8) in pancreatic beta-cells promotes cell survival-(Peer Reviewed Journal)
  Huang, L., Kirschke, C.P. 2016. Down-regulation of zinc transporter 8 (SLC30A8) in pancreatic beta-cells promotes cell survival. Austin Journal of Endocrinology and Diabetes. 3:1037.
- Down-regulation of zinc transporter 8 (SLC30A8) in pancreatic beta-cells promotes cell survival.-(Peer Reviewed Journal)
  Huang, L., Kirschke, C.P. 2016. Down-regulation of zinc transporter 8 (SLC30A8) in pancreatic beta-cells promotes cell survival.. Austin Journal of Endocrinology and Diabetes. 3(1):1-8.
- Zinc transporter 7 deficiency affects lipid synthesis in adipocytes by inhibiting insulin-dependent Akt activity and glucose uptake-(Peer Reviewed Journal)
  Tepaamorndech, S., Kirschke, C.P., Pedersen, T.L., Keyes, W.R., Newman, J.W., Huang, L. 2015. Zinc transporter 7 deficiency affects lipid synthesis in adipocytes by inhibiting insulin-dependent Akt activity and glucose uptake. FEBS Journal. 283(2):378-394. doi: 10.1111/febs.13582.
- Linking cellular zinc status to body weight and fat mass: mapping quantitative trait loci in Znt7 knockout mice -(Peer Reviewed Journal)
- Zinc -(Peer Reviewed Journal)
  Huang, L., Drake, V.J., Ho, E. 2015. Zinc. Advances in Nutrition. 6:224-226.
- Zinc and its transporters, pancreatic beta cells, and insulin metabolism-()
  Huang, L. 2014. Zinc and its transporters, pancreatic beta cells, and insulin metabolism. In: Litwack, G. editor. Vitamins and Hormones. Atlanta, GA: Elsevier. p.365-390.
- The SLC30 family of zinc transporters – a review of current understanding of their biological and pathophysiological roles-(Peer Reviewed Journal)
  Huang, L., Tepaamorndech, S. 2013. The SLC30 family of zinc transporters – a review of current understanding of their biological and pathophysiological roles. European Journal of Applied Physiology. 34(2-3):548-560.
- Dietary strawberry powder reduces blood glucose concentrations in obese and lean C57BL/6 mice and selectively lowers plasma C-reactive protein in lean mice-(Peer Reviewed Journal)
  Parelman, M.A., Storms, D.H., Kirschke, C.P., Huang, L., Zunino, S.J. 2012. Dietary strawberry powder reduces blood glucose concentrations in obese and lean C57BL/6 mice and selectively lowers plasma C-reactive protein in lean mice. British Journal of Nutrition. 1-11. DOI: 10.1017/S0007114512000037.
- Diet-influenced chromatin modification and expression of chemopreventive genes by the soy peptide, lunasin-()
  Galvez, A., Huang, L., Magbanua, M.M., Dawson, K., Nandi, S., Rodriguez, R.L. 2011. Diet-influenced chromatin modification and expression of chemopreventive genes by the soy peptide, lunasin. In: Bidlack, W.R., Rodriguez, R.L., editors. Nutritional Genomics: The Impact of Dietary Regulation of Gene Function on Human Disease. Taylor and Francis Group. 25-40.
- A null-mutation in the Znt7 gene accelerates prostate tumor formation in a transgenic adenocarcinoma mouse prostate model -(Peer Reviewed Journal)
  Tepaamorndech, S., Huang, L., Kirschke, C.P. 2011. A null-mutation in the Znt7 gene accelerates prostate tumor formation in a transgenic adenocarcinoma mouse prostate model. Cancer Letters. 308 (2011) 33-42.
- Over-expression of ZnT7 increases insulin synthesis and secretion in pancreatic beta-cells by promoting insulin gene transcription-(Peer Reviewed Journal)
  Huang, L., Mi, Y., Kirschke, C.P. 2010. Over-expression of ZnT7 increases insulin synthesis and secretion in pancreatic beta-cells by promoting insulin gene transcription. Experimental Cell Research. 2010 Volume 316, Issue 16 2630-2643
- Expression of ZNT (SLC30) family members in the epithelium of the mouse prostate during sexual maturation-(Peer Reviewed Journal)
  Kirschke, C.P., Huang, L. 2008. Expression of ZNT (SLC30) family members in the epithelium of the mouse prostate during sexual maturation. Journal of Histochemistry and Cytochemistry (2008) 39:359-370.
- Expression of zinc transporter ZnT7 in mouse superior cervical ganglion -(Peer Reviewed Journal)
  Gao, H., Xu, H., Wang, X., Dahlstrom, A., Huang, L., Wang, Z. 2008. Expression of zinc transporter ZnT7 in mouse superior cervical ganglion. Autonomic Neuroscience: Basic & Clinical, 140 (2008) 59-65.
- The cellular and subcellular localization of zinc transporter 7 in the mouse spinal cord-(Peer Reviewed Journal)
  Chi, Z., Ren, H., Wang, X., Rong, M., Huang, L., Wang, Z. 2008. The cellular and subcellular localization of zinc transporter 7 in the mouse spinal cord. Histology and Histopathology Cellular and Molecular Biology, Vol.23:781-787
- Abundant Expression of Zinc Transporters in the Amyloid Plaques of Alzheimer’s Disease Brain-(Peer Reviewed Journal)
  Zhang, L., Wang, X., Stoltenberg, M., Danscher, G., Huang, L., Wang, Z. 2008. Abundant Expression of Zinc Transporters in the Amyloid Plaques of Alzheimer’s Disease Brain. Elsevier. Brain Research Bulletin 77 (2008) 55-60.
- ALTERED EXPRESSION AND DISTRIBUTION OF ZINC TRANSPORTERS IN APP/PS1 TRANSGENIC MOUSE BRAIN-(Peer Reviewed Journal)
  Zhang, L., Wang, X., Zheng, Z., Ren, H., Stoltenberg, M., Danscher, G., Huang, L., Rong, M., Wang, Z. 2008. Altered expression and distribution of zinc transporters in app/ps1 transgenic mouse brain. Elsevier, Journal Neurobiology of Aging, Doi:10,1016:1-14, 2008.
- Znt7 (Slc30a7)-DEFICIENT MICE DISPLAY REDUCED BODY ZINC STATUS AND BODY FAT ACCUMULATION-(Peer Reviewed Journal)
  Huang, L., Yu, Y., Kirschke, C.P., Gertz, E.R., Kent K.C., L. 2008. Znt7 (Slc30a7)-Deficient Mice Display Rreduced Body ZincC Status and Body Fat Accumulation. Journal of Biological Chemistry. 282,51:37053-37063, 2007.
- Imunoreactivity of zinc transporter 7 (ZNT7) in mouse dorsal root ganglia-(Peer Reviewed Journal)
  Zhang, L., Chi, Z., Ren, H., Rong, M., Dahlstrom, A., Huang, L., Wang, Z. 2007. Imunoreactivity of zinc transporter 7 (ZNT7) in mouse dorsal root ganglia. Elsevier. Brain Research Bulletin 74 (2007) 278-283.
- A DI-LEUCINE SORTING SIGNAL IN ZIP1 (SLC39A1) MEDIATES ENDOCYTOSIS OF THE PROTEIN-(Peer Reviewed Journal)
  Huang, L., Kirschke, C.P. A di-leucine sorting signal in zip1 (slc39a1) mediates endocytosis of the protein. FEDERATION OF EUROPEAN BIOCHEMICAL SOCIETY, 274: 3986-3997, 2007.
- Localization of ZnT7 and zinc ions in mouse retina-Immunohistochemistry and selenium autometallography-()
  Wang, X., Wang, Z., Gao, H., Danscher, G., Huang, L. Localization of ZnT7 and zinc ions in mouse retina-Immunohistochemistry and selenium autometallography. Brain Research Bulletin, 2006.71:91-96
- IMMUNOHISTOCHEMICAL ANALYSIS OF ZNT1, 4, 5, 6, AND 7 IN THE MOUSE GASTROINTESTINAL TRACT-(Peer Reviewed Journal)
  Yu, Y.Y., Kirschke, C.P., Huang, L. 2006. IMMUNOHISTOCHEMICAL ANALYSIS OF ZNT1, 4, 5, 6, AND 7 IN THE MOUSE GASTROINTESTINAL TRACT. Journal of Histochemistry and Cytochemistry. Vol. 55(3):223-234, 2006.
- Zinc transporter 7 is located in the cis-Golgi apparatus of mouse choroid epithelial cells-(Peer Reviewed Journal)
  Chi, Z., Wang, X., Wang, Z., Gao, H., Dahlstrom, A., Huang, L. 2006. Zinc transporter 7 is located in the cis-Golgi apparatus of mouse choroid epithelial cells. NeuroReport. 2006. Vol.17:1807-1811.
- DECREASED INTRACELLULAR ZINC IN HUMAN TUMORIGENIC PROSTATE EPITHELIAL CELLS: A POSSIBLE ROLE IN PROSTATE CANCER PROGRESSION-(Peer Reviewed Journal)
  Huang, L., Kirschke, C.P., Zhang, Y. Decreased intracellular zinc in human tumorigenic prostate epithelial cells: a possible role in prostate cancer progression. Cancer Cell International 2006, 6:10, 2006.
- NUTRIENT-GENE INTERACTIONS INVOLVING SOY PEPTIDE AND CHEMOPREVENTIVE GENES IN PROSTATE EPITHELIAL CELLS-(Peer Reviewed Journal)
  Magvanua, M.J., Dawson, K., Huang, L., Malyj, W., Gregg, J., Galvez, A.F., Rodriguez, R.L. 2006. NUTRIENT-GENE INTERACTIONS INVOLVING SOY PEPTIDE AND CHEMOPREVENTIVE GENES IN PROSTATE EPITHELIAL CELLS. John Wiley & Sons, Inc. Chapter 11:255-276.
- ZIP7 GENE (SLC39A7) ENCODES A ZINC TRANSPORTER INVOLVED IN ZINC HOMEOSTASIS OF THE GOLGI APPARATUS-(Peer Reviewed Journal)
  Huang, L., Kirschke, C.P., Zhang, Y., Yu, Y. 2005. ZIP7 GENE (SLC39A7) ENCODES A ZINC TRANSPORTER INVOLVED IN ZINC HOMEOSTASIS OF THE GOLGI APPARATUS. Journal of Biological Chemistry 280(15):15456-15463, 2005.
- ABUNDANT EXPRESSION OF ZINC TRANSPORTERS IN BERGMAN GLIA OF MOUSE CEREBELLUM-(Peer Reviewed Journal)
  Wang, Z., Stoltenberg, M., Huang, L., Danscher, G., Dahlstrom, A., Shi, Y., Li, J. 2004. ABUNDANT EXPRESSION OF ZINC TRANSPORTERS IN BERGMAN GLIA OF MOUSE CEREBELLUM. Elsevier. Bulletin 64:441-44, 2004.
- INVESTIGATION OF LYMPHOCYTE GENE EXPRESSION FOR USE AS BIOMARKERS FOR ZINC STATUS IN HUMANS-(Peer Reviewed Journal)
  Andree, K.B., Kim, J., Kirschke, C.P., Gregg, J.P., Paik, H., Joung, H., Woodhouse, L.R., King, J., Huang, L. 2004. Investigation of lymphocyte gene expression for use as biomarkers for zinc status in humans. Journal of Nutrition. Journal of Nutrition. 134:1716-1723, 2004.
- DYNAMIC ZINC POOLS IN MOUSE CHOROID PLEXUS-(Peer Reviewed Journal)
  Wang, Z., Stoltenberg, M., Jo, S.M., Huang, L., Larsen, A., Dahlstrom, A., Danscher, G. 2004. Dynamic zinc pools in mouse choroid plexus. NeuroReport, 15:1801-1804, Lippincott, Williams & Wilkins.
- ZNT7, A NOVEL MAMMALIAN ZINC TRANSPORTER, ACCUMULATES ZINC IN THE GOLGI APPARATUS-(Peer Reviewed Journal)
  Kirschke, C.P., Huang, L. 2004. Znt7, a novel mammalian zinc transporter, accumulates zinc in the golgi apparatus. Journal of Biological Chemistry. 278:4096-103, 2004.
- EFFLUX AND COMPARTMENTALIZATION OF ZINC BY MEMBERS OF THE SLC30 FAMILY OF SOLUTE CARRIERS-(Peer Reviewed Journal)
  Palmiter, R.D., Huang, L. 2004. Efflux and compartmentalization of zinc by members of the slc30 family of solute carriers. Journal of Physiology (2004) 447:744-51.

- Methods and Technique
  
  Molecular biology methods (DNA/RNA isolation, PCR, Western blots, quantitative PCR

U.S. DEPARTMENT OF AGRICULTURE

Obesity and Metabolism Research: Davis, CA

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