| RT Table 2 |
|
TABLE 2. Cycle threshold (Ct) values for 25 isolates of Phytophthora ramorum, Phytophthora pseudosyringae and other Phytophthora species subjected to real-time PCR analysis.
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| Ct valuea | |
P. ramorum | P. ramorum primers and probe | P. pseudosyringae primers and probe |
Coen | 28.91 ? 0.44 | >60 ? 0b |
201C | 26.40 ? 0.13 | >60 ? 0 |
0-13 | 24.84 ? 0.46 | >60 ? 0 |
0-16 | 26.83 ? 0.56 | >60 ? 0 |
0-217 | 25.23 ? 0.11 | >60 ? 0 |
288 | 28.81 ? 0.05 | >60 ? 0 |
C | 27.40 ? 0.75 | >60 ? 0 |
73101 | 25.41? 0.55 | >60 ? 0 |
044519 | 25.26 ? 0.13 | >60 ? 0 |
044522 | 25.28? 0.44 | >60 ? 0 |
Prn-1 | 25.66 ? 0.15 | >60 ? 0 |
Prn-2 | 28.45 ? 0.69 | >60 ? 0 |
Prn-3 | 28.87 ? 0.14 | >60 ? 0 |
Prn-4 | 27.25 ? 0.01 | >60 ? 0 |
Prn-5 | 26.73 ? 1.12 | >60 ? 0 |
Prn-6 | 26.68 ? 0.18 | >60 ? 0 |
Prg-1 | 26.88 ? 0.21 | >60 ? 0 |
Prg-2 | 22.56 ? 0.11 | >60 ? 0 |
Prg-3 | 24.86 ? 0.14 | >60 ? 0 |
Prg-4 | 27.07 ? 0.18 | >60 ? 0 |
Prg-5 | 27.49 ? 0.27 | >60 ? 0 |
Prg-6 | 25.02 ? 0.15 | >60 ? 0 |
Prg-7 | 28.53 ? 0.42 | >60 ? 0 |
Prg-8 | 24.37 ? 0.52 | >60 ? 0 |
P72648 | 25.66 ? 0.76 | >60 ? 0 |
P. pseudosyringae | | |
470 | >60 ? 0 | 25.41? 0.03 |
471 | >60 ? 0 | 25.01? 0.40 |
472 | >60 ? 0 | 24.52 ? 0.64 |
473 | >60 ? 0 | 24.11 ? 0.06 |
484 | >60 ? 0 | 27.74 ? 0.33 |
485 | >60 ? 0 | 24.93 ? 0.25 |
Other Phytophthora speciesc | >60 ? 0 | >60 ? 0 |
negative control | >60 ? 0 | >60 ? 0 |
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a Data are mean values of two replicated experiments ? standard error.
b No fluorescence was detected at 60 cycles of PCR amplification when tested at a concentration of 100 pg DNA.
c Other species listed in Table 1.
