RT Table 3

TABLE 3.  Amount of DNA estimated to be present in dilutions of DNA extracted from Rhododendron sp. (cv. 'Cunningham's White') leaf disks infected with Phytophthora ramorum.

Dilution from Bio101 kita	Ct avgb ? SE	Amt. DNA calculated from standard curve
1:10	27.75 ? 0.32	20.9 pg
1:100	32.06 ? 0.53	1.4 pg
1:1000	35.37 ? 0.62	177 fg
1:10,000	39.57 ? 0.33	13 fg
1:100,000	42.81 ? 0.58	1.7 fg
1:1,000,000	55.34 ? 2.95	NDc

^a  DNA was extracted from two 6-mm diameter leaf disks using a Qbiogene Fast DNA extraction kit according to manufacturer's instructions.

^b Ct values are means of six observations, plus or minus the standard error.  Three separate extractions were performed (each using two 6-mm diameter leaf disks), and two replicate real-time PCR experiments were conducted, each containing sample from all three extractions diluted as indicated (n = 6).

^c ND = not determined due to out of range of the standard curve