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ARS Home » Pacific West Area » Tucson, Arizona » Carl Hayden Bee Research Center » Research » Varroa » How to Monitor, Sample, and Treat for Varroa » Sugar Shake

1 - How to Monitor, Sample, and Treat for Varroa
2 - When to Sample
3 - Sugar Shake
4 - Alcohol Wash
5 - Sticky Board
6 - Disclaimer

USDA Varroa Sugar Shake Instructions
How to Sample for Mites

Materials needed:
Mason jar outfitted with ⅛ inch hardware cloth (cut to fill the ring of the lid)
Bee brush
Measuring cup (½ or 1 cup)
Tablespoon
Powdered sugar
White / light colored tray or tub
Water

  1. Place 1 tablespoon of powdered sugar into the screened jar. Leave the lid off.
  2. Select a brood frame with plenty of bees on it. Make sure the queen is not on the frame.
  3. Hold the measuring cup flush with the frame, and using a bee brush, gently brush the
    bees into the measuring cup, filling to ½ cup. Once the bees are in the cup, the cup can
    be shaken back and forth to get the bees to settle for an accurate measurement. If
    necessary, add more bees.
  4. Quickly dump the bees into the mason jar and place the screened lid on tight.
  5. Roll or shake the jar to evenly coat the bees with powdered sugar. Let it sit in the shade
    for 1 minute. (DO NOT leave the bees in the sun or in the jar too long, as they may get
    sticky and/or asphyxiate).
  6. Fill a white / light colored tub or tray with 1 inch of water. Vigorously shake the jar upside
    down until there is no more powdered sugar coming out (approximately 30 seconds to 1
    minute). This will cause the mites to dislodge from the bees and fall into the water. The
    sugar will dissolve in the water, making the mites easier to see.
  7. Return the sugared bees to the hive. Their sisters will clean them.
  8. Count the number of varroa mites in the tray (reddish brown ovals).
  9. ½ cup of bees is approximately 300 bees. To calculate mites per 100 bees, divide the
    total mites found in the sample by 3.
    ** Treatment is recommended if the sugar shake or alcohol wash results in 3 mites per 100
    bees

Sugar shake supplies
/ARSUserFiles/20220500/Varroa/Sugar Shake/1 Sugar shake supplies.png

Select a brood frame with plenty of bees
/ARSUserFiles/20220500/Varroa/Sugar Shake/2 Select a brood frame with plenty of bees.png

Add 1 tablespoon of powdered sugar to mason jar
/ARSUserFiles/20220500/Varroa/Sugar Shake/3 Add 1 tablespoon of powdered sugar to mason jar.png

Brush approximately 1/2 cup of bees into measuring cup
/ARSUserFiles/20220500/Varroa/Sugar Shake/4 Brush approx 1 and half cup of bees into measuring cup.png

Shake the measuring cup back and forth to help the bees settle and visually determine whether there are enough bees
/ARSUserFiles/20220500/Varroa/Sugar Shake/5 shake the measuring cup back and forth to help the bees settle and visually determine.png

Pour the bees into the jar with the powdered sugar
/ARSUserFiles/20220500/Varroa/Sugar Shake/6 Pour the bees into the jar with the powdered sugar.png

Add the hardware cloth lid and roll the jar evenly coats the bees with sugar
/ARSUserFiles/20220500/Varroa/Sugar Shake/7 Add the hardware cloth lid and roll the jar evenely coast the bees with sugar.png

Bees coated with powdered sugar
/ARSUserFiles/20220500/Varroa/Sugar Shake/8 Bees coated with powdered sugar.png

Add 1 inch of water to a white or light colored tub
/ARSUserFiles/20220500/Varroa/Sugar Shake/9 Add 1 inch of water to a white or light colored tub.png

Vigorously shake the jar up and down until all the powdered sugar comes off (approximately 30 seconds to 1 minute)
/ARSUserFiles/20220500/Varroa/Sugar Shake/10 vigorously shake the jar up and down until all powdered sugar come off.png

Count the varroa mites in the water
/ARSUserFiles/20220500/Varroa/Sugar Shake/11 Count the varroa mites in the water.png

Credit: All Photos taken by Mona Chambers

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