Skip to main content
ARS Home » People & Locations » Manuel Borca
Manuel Borca (PhD)
Foreign Animal Disease Research
Research Microbiologist

Phone:
Fax:

(Employee information on this page comes from the REE Directory. Please contact your front office staff to update the REE Directory.)

Projects
Supporting African Swine Fever and Classical Swine Fever Research
Cooperative Agreement (A)
  Accession Number: 445017
Intervention Strategies to Control and Eradicate Foreign Animal Diseases of Swine
In-House Appropriated (D)
  Accession Number: 441308
Intervention Strategies to Respond, Control, and Eradicate Foot-and-Mouth Disease Virus (FMDV)
In-House Appropriated (D)
  Accession Number: 441364
The Global African Swine Fever Research Alliance (GARA)
Memorandum of Understanding (M)
  Accession Number: 441056
Safety and Efficacy Assessment of African Swine Fever Vaccines For Use in the Dominican Republic
Memorandum of Understanding (M)
  Accession Number: 445907
African Swine Fever Collaborative Agreement
Non-Funded Cooperative Agreement (N)
  Accession Number: 440732
Production of Master Seed Virus for Development of Vaccine Against African Swine Fever
Non-Assistance Cooperative Agreement (S)
  Accession Number: 445178
Classical Swine Fever (CSF) Vaccine FlagT4G Vaccine Efficacy: Assessing Oral Administration and Protection in Pregnant Sows
Non-Assistance Cooperative Agreement (S)
  Accession Number: 445844
Analysis of the Cross Protective Response Induced by ASFV Recombinant Vaccine ASFV-G-DeltaI177L
Non-Assistance Cooperative Agreement (S)
  Accession Number: 444757
Development of Safe Vaccination Protocols for Pregnant Sows, Piglets and Boars Using ASFV-G-DI177L
Non-Assistance Cooperative Agreement (S)
  Accession Number: 444906
Enhancing Capacity for Veterinary Pathology
Non-Assistance Cooperative Agreement (S)
  Accession Number: 444930
Production of GMP-compliant Master Seed Virus (MSV) for Development of Vaccine Against African Swine Fever (ASF)
Non-Assistance Cooperative Agreement (S)
  Accession Number: 445010
Structural Characterization of African Swine Fever Virus (ASFV) Membrane Interacting Proteins
Non-Assistance Cooperative Agreement (S)
  Accession Number: 446239
Analysis of the Immunogenicity of ASFV Proteins
Non-Assistance Cooperative Agreement (S)
  Accession Number: 446437
Reversion to Virulene Studies for African Swine Fever Vaccines
Non-Assistance Cooperative Agreement (S)
  Accession Number: 446496
Characterizing the Structure-Function of Various Families of Viroporins to Support African Swine Fever Virus Vaccine Candidates
Non-Assistance Cooperative Agreement (S)
  Accession Number: 438233
Analysis of the Immune Response Elicited by Classical Swine Fever Virus Recombinant Vaccine Candidate FlagT4G
Non-Assistance Cooperative Agreement (S)
  Accession Number: 438599
Functional Genomics of African Swine Fever Virus Infections - An Effective System to Identify Pro-Tolerance and Resilience Pathways in Pigs
Non-Assistance Cooperative Agreement (S)
  Accession Number: 440094
Evaluation of the Modification of Virulence in Recombinant ASFV with Specific Gene Deletions
Non-Assistance Cooperative Agreement (S)
  Accession Number: 440174
Curated African Swine Fever Genomic Database
Non-Assistance Cooperative Agreement (S)
  Accession Number: 442398
Development of a Differential ELISA for ASV Vaccines
Non-Assistance Cooperative Agreement (S)
  Accession Number: 442399
Development of an ASFV Nano-biosensor for Discriminating Between Infected and Vaccinated Animals
Non-Assistance Cooperative Agreement (S)
  Accession Number: 442814
Understanding Molecular Bases in the Production of Genetic Diversity of African Swine Fever Virus
Non-Assistance Cooperative Agreement (S)
  Accession Number: 442906

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Deletion of the EP402R gene from the genome of African swine fever vaccine strain ASFV-G-deltaI177L provides the potential capability of differentiating between infected and vaccinated animals Reprint Icon - (Peer Reviewed Journal)
Borca, M.V., Ramirez Medina, E., Espinoza, N.N., Rai, A., Spinard III, E.J., Velazquez Salinas, L., Valladares, A., Silva, E.B., Burton, L.J., Meyers, A., Clark, J.A., Wu, P., Gay, C.G., Gladue, D.P. 2024. Deletion of the EP402R gene from the genome of African swine fever vaccine strain ASFV-G-deltaI177L provides the potential capability of differentiating between infected and vaccinated animals. Viruses. 16(3). Article 376. https://doi.org/10.3390/v16030376.
Near-complete genome sequences of multiple genotype I African swine fever virus isolates from 2016 to 2018 in Cameroon Reprint Icon - (Peer Reviewed Journal)
Spinard III, E.J., Wade, A., Unger, H., Nenkam, R., Mayega, F.J., Sreenu, V., Da Silva Filipe, A., Mair, D., Borca, M.V., Gladue, D.P., Mesambe, C. 2024. Near-complete genome sequences of multiple genotype I African swine fever virus isolates from 2016 to 2018 in Cameroon. Microbiology Resource Announcements. 13. Article e00978-23. https://doi.org/10.1128/mra.00978-23.
African swine fever virus p72 identification tool Reprint Icon - (Other)
Dinhobl, M.W., Spinard III, E.J., Birtley, H., Tesler, N., Masembe, C., Borca, M.V., Gladue, D.P. 2024. African swine fever virus p72 identification tool. Microbiology Resource Announcements. 13(2). Article e00891-23. https://doi.org/10.1128/mra.00891-23.
Evaluation of the deletion of the African swine fever virus gene O174L from the genome of the Georgia isolate Reprint Icon - (Peer Reviewed Journal)
Ramirez Medina, E., Velazquez Salinas, L., Rai, A., Espinoza, N.N., Valladares, A., Silva, E.B., Burton, L.J., Spinard III, E.J., Meyers, A., Risatti, G., Calvelage, S., Blome, S., Gladue, D.P., Borca, M.V. 2023. Evaluation of the deletion of the African swine fever virus gene O174L from the genome of the Georgia isolate. Viruses. 15(10). Article 2134. https://doi.org/10.3390/v15102134.
African swine fever vaccine candidate ASFV-g-deltaI177l produced in the swine macrophage-derived cell line IPKM remains genetically stable and protective against homologous virulent challenge Reprint Icon - (Peer Reviewed Journal)
Borca, M.V., Rai, A., Espinoza, N.N., Ramirez Medina, E., Spinard III, E.J., Velazquez Salinas, L., Valladares, A., Silva, E.B., Burton, L.J., Meyers, A., Gay, C.G., Gladue, D.P. 2023. African swine fever vaccine candidate ASFV-g-deltaI177l produced in the swine macrophage-derived cell line IPKM remains genetically stable and protective against homologous virulent challenge. Viruses. 5(10). Article 2064. https://doi.org/10.3390/v15102064.
African swine fever vaccine candidate ASFV-G-deltaI177L produced in the swine macrophage-derived cell line IPKM remains genetically stable and protective against homologous virulent challenge Reprint Icon - (Peer Reviewed Journal)
Ramirez Medina, E., Rai, A., Espinoza, N.N., Spinard III, E.J., Meyers, A., Valladares, A., Velazquez Salinas, L., Gay, C.G., Gladue, D.P., Borca, M.V., Silva, E.B., Burton, L.J., Clark, J.A. 2023. African swine fever vaccine candidate ASFV-G-deltaI177L produced in the swine macrophage-derived cell line IPKM remains genetically stable and protective against homologous virulent challenge. Pathogens. 15(10). Article 2064. https://doi.org/10.3390/v15102064.
A re-evaluation of African swine fever genotypes based on p72 sequences reveals only 5 distinct p72 groups Reprint Icon - (Peer Reviewed Journal)
Spinard III, E.J., Dinhobl, M.W., Tesler, N., Birtley, H., Signore, A., Ambagala, A., Masembe, C., Borca, M.V., Gladue, D.P. 2023. A re-evaluation of African swine fever genotypes based on p72 sequences reveals only 5 distinct p72 groups. Viruses. 15(8). Article 1722. https://doi.org/10.3390/v15081722.
The 2022 outbreaks of African swine fever virus demonstrate the first report of genotype II in Ghana Reprint Icon - (Peer Reviewed Journal)
Spinard III, E.J., Rai, A., Osei-Bonsu, J., O'Donnell, V., Ababio, P., Tawuah-Yingar, D., Baah, D., Ramirez Medina, E., Espinoza, N.N., Valladares, A., Faburay, B., Ambagala, A., Odoom, T., Borca, M.V., Gladue, D.P. 2023. The 2022 outbreaks of African swine fever virus demonstrate the first report of genotype II in Ghana. Viruses. 15(8). Article 1722. https://doi.org/10.3390/v15081722.
ASF vaccine candidate ASFV-G-deltaI177l does not exhibit residual virulence in long-term clinical studies Reprint Icon - (Peer Reviewed Journal)
Borca, M.V., Ramirez Medina, E., Silva, E.B., Ayushi, R., Espinoza, N.N., Velazquez Salinas, L., Gladue, D.P. 2023. ASF vaccine candidate ASFV-G-deltaI177l does not exhibit residual virulence in long-term clinical studies. Pathogens. 12(6). https://doi.org/10.3390/pathogens12060805.
Confirming the absence of parental African swine fever virus as a potential contaminant of recombinant live attenuated ASF vaccines Reprint Icon - (Peer Reviewed Journal)
Velazquez Salinas, L., Ramirez Medina, E., Rai, A., Pruitt, S.E., Vuono, E., Espinoza, N.N., Gay, C.G., Witte, S.B., Gladue, D.P., Borca, M.V. 2023. Confirming the absence of parental African swine fever virus as a potential contaminant of recombinant live attenuated ASF vaccines. Biologicals. 3;83:101685. https://doi.org/10.1016/j.biologicals.2023.101685.
Pathogenesis and transmissibility studies of the ASFV strain currently causing outbreaks in the Dominican Republic Reprint Icon - (Abstract Only)
Ramirez Medina, E., O'Donell, V., Silva, E.B., Espinoza, N.N., Velazquez Salinas, L., Moran, K., Daite, D., Barrette, R., Bonto, F., Holland, R., Gladue, D.P., Borca, M.V. 2023. Pathogenesis and transmissibility studies of the ASFV strain currently causing outbreaks in the Dominican Republic. Meeting Abstract. https://doi.org/10.3390/v14051090.
Classical swine fever virus structural glycoprotein E2 interacts with host protein ACADM during the virus infectious cycle Reprint Icon - (Peer Reviewed Journal)
Vuono, E., Ramirez Medina, E., Silva, E.B., Berggren, K., Rai, A., Espinoza, N.N., Gladue, D.P., Borca, M.V. 2023. Classical swine fever virus structural glycoprotein E2 interacts with host protein ACADM during the virus infectious cycle. Viruses. 15(5). https://doi.org/10.3390/v15051036.
Development of porcine monoclonal antibodies with in vitro neutralizing activity against classical swine fever virus from C-strain E2-specific single B cells Reprint Icon - (Peer Reviewed Journal)
Wang, L., Madera, R., Li, Y., Gladue, D.P., Borca, M.V., McIntosh, M., Shi, J. 2023. Development of porcine monoclonal antibodies with in vitro neutralizing activity against classical swine fever virus from C-strain E2-specific single B cells. Viruses. 15(4). Article 863. https://doi.org/10.3390/v15040863.
Evaluation of the function of ASFV gene E66L in the process of virus replication and virulence in swine Reprint Icon - (Peer Reviewed Journal)
Ramirez Medina, E., Vuono, E., Rai, A., Espinoza, N.N., Valladares, A., Spinard III, E.J., Velazquez Salinas, L., Gladue, D.P., Borca, M.V. 2023. Evaluation of the function of ASFV gene E66L in the process of virus replication and virulence in swine. Viruses. 15(2). Article 566. https://doi.org/10.3390/v15020566.
Whole genome sequencing and phylogenetic analysis of African swine fever virus detected from a backyard pig in Mongolia, 2019 Reprint Icon - (Peer Reviewed Journal)
Hyeon, J., Tseren-Ochir, E., Lee, D., Gladue, D.P., Borca, M.V., Risatti, G.R. 2023. Whole genome sequencing and phylogenetic analysis of African swine fever virus detected from a backyard pig in Mongolia, 2019. Viruses. 10. https://doi.org/10.3389/fvets.2023.1094052.
Full genome sequence for the African swine fever virus outbreak in the Dominican Republic in 1980 Reprint Icon - (Peer Reviewed Journal)
Spinard III, E.J., O'Donnell, V., Vuono, E., Davis, C., Rai, A., Ramirez Medina, E., Espinoza, N.N., Valladares, A., Borca, M.V., Gladue, D.P. 2023. Full genome sequence for the African swine fever virus outbreak in the Dominican Republic in 1980. Scientific Reports. 13. Article 1024. https://doi.org/10.1038/s41598-022-25987-5.
A highly effective African swine fever virus vaccine elicits a memory T cell response in vaccinated swine Reprint Icon - (Peer Reviewed Journal)
Attreed, S.E., Silva, C.M., Abbott, S.T., Ramirez Medina, E., Espinoza, N.N., Borca, M.V., Gladue, D.P., Diaz San Segundo, F.C. 2022. A highly effective African swine fever virus vaccine elicits a memory T cell response in vaccinated swine. Pathogens. 11(12). Article 1438. https://doi.org/10.3390/pathogens11121438.
Deletion of an African swine fever ATP-dependent RNA hel-icase QP509L from the highly virulent Georgia 2010 strain does not affect replication or virulence Reprint Icon - (Peer Reviewed Journal)
Ramirez Medina, E., Vuono, E., Pruitt, S., Rai, A., Espinoza, N.N., Spinard III, E.J., Valadares, A., Silva, E.B., Velazquez Salinas, L., Borca, M.V., Gladue, D.P. 2022. Deletion of an African swine fever ATP-dependent RNA hel-icase QP509L from the highly virulent Georgia 2010 strain does not affect replication or virulence. Viruses. 14(11). https://doi.org/10.3390/v14112548.
The presence of virus neutralizing antibodies is highly associated with protection against virulent challenge in domestic pigs immunized with ASFV live attenuated vaccine candidates Reprint Icon - (Peer Reviewed Journal)
Silva, E.B., Krug, P., Ramirez Medina, E., Pruitt, S., Rai, A., Espinoza, N.N., Valladares, A., Gladue, D.P., Borca, M.V. 2022. The presence of virus neutralizing antibodies is highly associated with protection against virulent challenge in domestic pigs immunized with ASFV live attenuated vaccine candidates. Pathogens. 11(11). Article 1311. https://doi.org/10.3390/pathogens11111311.
Structural predictions of the complete ASFV-G proteome Reprint Icon - (Peer Reviewed Journal)
Spinard III, E.J., Azzinaro, P.A., Rai, A., Espinoza, N.N., Ramirez Medina, E., Borca, M.V., Gladue, D.P. 2022. Structural predictions of the complete ASFV-G proteome. Microbiology Resource Announcements. 11(12):e0088122. https://doi.org/10.1128/mra.00881-22.
The FlagT4G vaccine confers a strong and regulated innate immunity that correlates with early virological protection against Classical swine fever Reprint Icon - (Peer Reviewed Journal)
Bohórquez, J.A., Wang, M., Diaz, I., Alberch, M., Perez-Simo, M., Rosell, R., Gladue, D.P., Borca, M.V., Llilianne, G. 2022. The FlagT4G vaccine confers a strong and regulated innate immunity that correlates with early virological protection against classical swine fever. Viruses. 14(9). https://doi.org/10.3390/v14091954.
ASFV Gene A151R is involved in the process of virulence in domestic swine Reprint Icon - (Peer Reviewed Journal)
Ramirez Medina, E., Vuono, E., Pruitt, S., Rai, A., Espinoza, N.N., Valladares, A., Spinard III, E.J., Silva, E.B., Velazquez Salinas, L., Gladue, D.P., Borca, M.V. 2022. ASFV Gene A151R is involved in the process of virulence in domestic swine. Viruses. 14(8). Article 1834. https://doi.org/10.3390/v14081834.
Deletion of the EP296R gene from the genome of highly virulent African swine fever virus Georgia 2010 does not affect virus replication or virulence in domestic pigs Reprint Icon - (Abstract Only)
Vuono, E., Ramirez Medina, E., Pruitt, S.E., Rai, A., Espinoza, N.N., Spinard Iii, E.J., Valladares, A., Velazquez Salinas, L., Gladue, D.P., Borca, M.V. 2022. Deletion of the EP296R gene from the genome of highly virulent African swine fever virus Georgia 2010 does not affect virus replication or virulence in domestic pigs. Virus Research. https://doi.org/10.3390/v14081682.
Deletion of the ASFV dUTPase gene E165R from the genome of highly virulent African swine fever virus Georgia 2010 does not affect virus replication or virulence in domestic pigs Reprint Icon - (Peer Reviewed Journal)
Vuono, E., Ramirez Medina, E., Pruitt, S.E., Rai, A., Espinoza, N.N., Silva, E.B., Velazquez Salinas, L., Gladue, D.P., Borca, M.V. 2022. Deletion of the ASFV dUTPase gene E165R from the genome of highly virulent African swine fever virus Georgia 2010 does not affect virus replication or virulence in domestic pigs. Viruses. 14(7). Article 1409. https://doi.org/10.3390/v14071409.
Determining hyperimmune serum neutralizing effect on African Swine Fever Virus and serum enhancing effect on extracellular virion infectivity in adherent pig PBMC by flow cytometry Reprint Icon - (Peer Reviewed Journal)
Canter, J.A., Aponte, T., Ramirez Medina, E., Pruitt, S.E., Neilan, J., Gladue, D.P., Borca, M.V., Zhu, J.J. 2022. Determining hyperimmune serum neutralizing effect on African Swine Fever Virus and serum enhancing effect on extracellular virion infectivity in adherent pig PBMC by flow cytometry. Scientific Reports. https://doi.org/10.3390/v14061249.
Determining hyperimmune serum neutralizing effect on African Swine Fever Virus and serum enhancing effect on extracellular virion infectivity in adherent pig PBMC by flow cytometryg Reprint Icon - (Abstract Only)
Canter, J.A., Aponte, T., Ramirez Medina, E., Pruitt, S.E., Gladue, D.P., Borca, M.V., Zhu, J.J. 2022. Determining hyperimmune serum neutralizing effect on African Swine Fever Virus and serum enhancing effect on extracellular virion infectivity in adherent pig PBMC by flow cytometryg. Meeting Abstract. https://doi.org/10.3390/v15040915.
Deletion of H108R reduces virulence of the Georgia strain of African swine fever virus with surviving animals being protected against virulent challenge Reprint Icon - (Peer Reviewed Journal)
Vuono, E., Ramirez Medina, E., Silva, E.B., Rai, A., Pruitt, S.E., Espinoza, N.N., Valladares, A., Velazquez Salinas, L., Gladue, D.P., Borca, M.V. 2022. Deletion of H108R reduces virulence of the Georgia strain of African swine fever virus with surviving animals being protected against virulent challenge. Journal of Virology. https://doi.org/10.1128/jvi.00545-22.
Experimental infection of domestic pigs with an African swine fever virus field strain isolated in 2021 from the Dominican Republic Reprint Icon - (Peer Reviewed Journal)
Ramirez Medina, E., O'Donnell, V., Silva, E.B., Espinoza, N.N., Velazquez Salinas, L., Gladue, D.P., Borca, M.V. 2022. Experimental infection of domestic pigs with an African swine fever virus field strain isolated in 2021 from the Dominican Republic. Viruses. https://doi.org/10.3390/v14051090.
Recombinant ASF live attenuated virus strains as experimental vaccine candidates Reprint Icon - (Peer Reviewed Journal)
Gladue, D.P., Borca, M.V. 2022. Recombinant ASF live attenuated virus strains as experimental vaccine candidates. Viruses. https://doi.org/10.3390/v14050878.
Evaluation of the safety profile of the ASFV vaccine candidate ASFV-G-I177L Reprint Icon - (Peer Reviewed Journal)
Xuan, T., Le Thi Thu, P., Nguyen Quang, H., Do Thanh, T., Nguyen Van, D., Pham Hào, Q., Quách Võ, N., Gay, C.G., Gladue, D.P., Borca, M.V. 2022. Evaluation of the safety profile of the ASFV vaccine candidate ASFV-G-I177L. Viruses. https://doi.org/10.3390/v14050896.
Evaluation of an ASFV RNA helicase gene A859L for virus replication and swine virulence Reprint Icon - (Peer Reviewed Journal)
Ramirez Medina, E., Vuono, E., Pruitt, S.E., Rai, A., Espinoza, N.N., Velazquez-Salinas, L., Gladue, D.P., Borca, M.V. 2021. Evaluation of an ASFV RNA helicase gene A859L for virus replication and swine virulence. Viruses. https://doi.org/10.3390/v14010010.
Optimization in the expression of ASFV proteins for the development of subunit vaccines using poxviruses as delivery vectors. Reprint Icon - (Peer Reviewed Journal)
Jaime, L., Lex, M., Gladue, D.P., Borca, M.V., Jorge, O. 2021. Optimization in the expression of ASFV proteins for the development of subunit vaccines using poxviruses as delivery vectors. Scientific Reports. https://doi.org/10.1038/s41598-021-02949-x.
Identification of genetic deletions in ASFV that could be potentially used as targets for the development of DIVA vaccines Reprint Icon - (Abstract Only)
Vuono, E., Ramirez Medina, E., Silva, E.B., Rai, A., Pruitt, S.E., Espinoza, N.N., Velazquez Salinas, L., Borca, M.V., Gladue, D.P. 2022. Identification of genetic deletions in ASFV that could be potentially used as targets for the development of DIVA vaccines. Meeting Abstract. https://doi.org/10.1128/JVI.01419-21.
Deletion of E184L, a Putative DIVA Target from the Pandemic Strain of African Swine Fever Virus, Produces a Reduction in Virulence and Protection against Virulent Challenge Reprint Icon - (Peer Reviewed Journal)
Ramirez Medina, E., Vuono, E., Rai, A., Pruitt, S.E., Espinoza, N.N., Velazquez Salinas, L., Pina-Pedrero, S., Zhu, J.J., Rodriguez, F., Borca, M.V., Gladue, D.P. 2021. Deletion of E184L, a putative DIVA target from the pandemic strain of African Swine Fever Virus, produces a reduction in virulence and protection against virulent challenge. Viruses. https://doi.org/10.1128/JVI.01419-21.
Development of a dendrimeric peptide-based approach for the differentiation of animals vaccinated with FlagT4G against classical swine fever from infected pigs Reprint Icon - (Peer Reviewed Journal)
Bohórquez, J., Defaus, S., Pérez-Simó, M., Alberch, M., Gladue, D.P., Borca, M.V., Andreu, D., Ganges, L. 2021. Development of a dendrimeric peptide-based approach for the differentiation of animals vaccinated with FlagT4G against classical swine fever from infected pigs. Viruses. https://doi.org/10.3390/v13101980.
Development real-time PCR assays to genetically differentiate vaccinated pigs from pigs infected with the Eurasian strain of African swine fever virus Reprint Icon - (Peer Reviewed Journal)
Velazquez Salinas, L., Ramirez Medina, E., Rai, A., Pruitt, S.E., Vuono, E.A., Espinoza, N.N., Gladue, D.P., Borca, M.V. 2021. Development real-time PCR assays to genetically differentiate vaccinated pigs from pigs infected with the Eurasian strain of African swine fever virus. Frontiers in Veterinary Science. https://doi.org/10.3389/fvets.2021.768869.
African swine fever virus vaccine candidate ASFV-G-¿I177L efficiently protects European and native pig breeds against circulating Vietnamese field strain. Reprint Icon - (Peer Reviewed Journal)
Tran, X., Le Thi Thu, P., Nguyen Quang, H., Do Thanh, T., Nguyen Van, D., Gay, C.G., Borca, M.V., Gladue, D.P. 2021. African swine fever virus vaccine candidate ASFV-G-I177L efficiently protects European and native pig breeds against circulating Vietnamese field strain. Transboundary and Emerging Diseases. https://doi.org//10.1111/tbed.14329.
Additional deletions to further attenuate BA71¿CD2 recombinant virus decreases vaccine efficacy without providing additional safety. - (Peer Reviewed Journal)
Development of a novel African swine fever virus (ASFV) live attenuated vaccine candidate: deletion of A137R gene drastically attenuates Georgia strain and induce protection against the virulent challenge Reprint Icon - (Peer Reviewed Journal)
Gladue, D.P., Ramirez Medina, E., Vuono, E., Silva, E.B., Rai, A., Pruitt, S.E., Espinoza, N.N., Velazquez-Salinas, L., Borca, M.V. 2021. Development of a novel African swine fever virus (ASFV) live attenuated vaccine candidate: deletion of A137R gene drastically attenuates Georgia strain and induce protection against the virulent challenge. Journal of Virology. https://doi.org/10.1128/JVI.01139-21.
Evaluation of the function of ASFV KP177R gene, encoding for structural protein p22, in the process of virus replication and in swine virulence Reprint Icon - (Peer Reviewed Journal)
Ramirez-Medina, E., Vuono, E., Pruitt, S.E., Rai, A., Espinoza, N.N., Velazquez-Salinas, L., Gladue, D.P., Borca, M.V. 2021. Evaluation of the function of ASFV KP177R gene, encoding for structural protein p22, in the process of virus replication and in swine virulence. Viruses. https://doi.org/10.3390/v13060986.
A cell culture-adapted vaccine virus against the current pandemic African swine fever virus strain Reprint Icon - (Peer Reviewed Journal)
Borca, M.V., Rai, A., Ramirez-Medina, E., Silva, E.B., Velazquez-Salinas, L., Vuono, E., Espinoza, N.N., Pruitt, S.E., Gladue, D.P. 2021. A cell culture-adapted vaccine virus against the current pandemic African swine fever virus strain. Nature Communications. https://doi.org/10.1128/JVI.00123-21.
ASFV-G-I177L is an effective oral nasal vaccine against the Eurasia Strain of Africa swine fever Reprint Icon - (Peer Reviewed Journal)
Borca, M.V., Ramirez Medina, E., Silva, E., Vuono, E., Rai, A., Pruitt, S.E., Gay, C.G., Espinoza, N.N., Velazquez-Salinas, L., Gladue, D.P. 2021. ASFV-G-I177L is an effective oral nasal vaccine against the Eurasia Strain of Africa swine fever. Viruses. https://doi.org/10.3390/v13050765.
Structural glycoprotein E2 of classical swine fever virus critically interacts with host protein Torsin-1A during the virus infectious cycle Reprint Icon - (Peer Reviewed Journal)
Vuono, E., Ramirez-Media, E., Velazquez-Salinas, L., Berggren, K., Rai, A., Pruitt, S.E., Espinoza, N.N., Gladue, D.P., Borca, M.V. 2021. Structural glycoprotein E2 of classical swine fever virus critically interacts with host protein Torsin-1A during the virus infectious cycle. Virology Journal. https://doi.org/10.1128/JVI.00314-21.
Development and in vivo evaluation of a recombinant African swine fever strain Georgia with a deletion in the MGF110-1L gene Reprint Icon - (Peer Reviewed Journal)
Ramirez-Medina, E., Vuono, E., Rai, A., Pruitt, S.E., Silva, E., Espinoza, N.N., Velazquez-Salinas, L., Zhu, J.J., Borca, M.V., Gladue, D.P. 2021. Development and in vivo evaluation of a recombinant African swine fever strain Georgia with a deletion in the MGF110-1L gene. Viruses. https://doi.org/10.3390/v13020286.
Evaluation of the deletion of ASFV MGF110-5L-6L on swine virulence and its potential use as a DIVA vaccine marker gene Reprint Icon - (Peer Reviewed Journal)
Ramirez Medina, E., Vuono, E., Pruitt, S.E., Rai, A., Valladares, A., Espinoza, N.N., Velazquez Salinas, L., Gladue, D.P., Borca, M.V. 2021. Evaluation of the deletion of ASFV MGF110-5L-6L on swine virulence and its potential use as a DIVA vaccine marker gene. Viruses. https://doi.org/10.3390/v13020286.
Evaluation in swine of a recombinant Georgia 2010 African Swine Fever Virus lacking the I8L Gene Reprint Icon - (Peer Reviewed Journal)
Vuono, E., Ramirez-Medina, E., Rai, A., Pruitt, S.E., Silva, E., Espinoza, N.N., Velazquez-Salinas, L., Zhu, J.J., Borca, M.V., Gladue, D.P. 2020. Evaluation in swine of a recombinant Georgia 2010 African Swine Fever Virus lacking the I8L Gene. Viruses. https://doi.org/10.3390/v13010039.
Deletion of CD2-like (CD2v) and C-type lectin-like (EP153R) genes from African swine fever virus Georgia- ¿9GL abrogates its effectiveness as an experimental vaccine Reprint Icon - (Peer Reviewed Journal)
Gladue, D.P., O'Donnell, V., Ramierez-Medina, E., Rai, A., Pruitt, S.E., Vuono, E., Silva, E., Velazquez-Salinas, L., Borca, M.V. 2020. Deletion of CD2-like (CD2v) and C-type lectin-like (EP153R) genes from African swine fever virus Georgia- 9GL abrogates its effectiveness as an experimental vaccine. Viruses. https://doi.org/10.3390/v12101185.
Evaluation in swine of a recombinant African swine fever virus lacking the MGF-360-1L gene Reprint Icon - (Peer Reviewed Journal)
Ramierez-Medina, E., Vuono, E., Rai, A., Pruitt, S.E., Silva, E., Velazquez-Salinas, L., Zhu, J.J., Gladue, D.P., Borca, M.V. 2020. Evaluation in swine of a recombinant African swine fever virus lacking the MGF-360-1L gene. Viruses. https://doi.org/10.3390/v12101193.
Positive Selection of ORF1ab, ORF3a, and ORF8 Genes Drives the Early Evolutionary Trends of SARS-CoV-2 During the 2020 COVID-19 Pandemic Reprint Icon - (Peer Reviewed Journal)
Velazquez-Salinas, L., Zarate, S., Eberl, S., Gladue, D.P., Novella, I., Borca, M.V. 2020. Positive selection of ORF1ab, ORF3a, and ORF8 genes drives the early evolutionary trends of SARS-CoV-2 during the 2020 COVID-19 pandemic. Frontiers in Microbiology. https://doi.org/10.3389/fmicb.2020.550674.
X69R is a non-essential gene that when deleted from African swine fever does not affect virulence in swine Reprint Icon - (Peer Reviewed Journal)
Ramirez-Medina, E., Vuono, E.A., Pruitt, S., Rai, A., Silva, E., Zhu, J.J., Velazquez-Salinas, L., Gladue, D.P., Borca, M.V. 2020. X69R is a non-essential gene that when deleted from African swine fever does not affect virulence in swine. Viruses. https://doi.org/10.3390/v12090918.
X69R is a non-essential gene that when deleted from African swine fever does not affect virulence in swine Reprint Icon - (Peer Reviewed Journal)
Ramirez-Medina, E., Vuono, E.A., Pruitt, S.E., Rai, A., Silva, E., Zhu, J.J., Velazquez-Salinas, L., Gladue, D.P., Borca, M.V. 2020. X69R is a non-essential gene that when deleted from African swine fever does not affect virulence in swine. Viruses. https://doi.org/10.3390/v12090918.
The C962R ORF of African swine fever strain Georgia is non-essential and not required for virulence in swine Reprint Icon - (Peer Reviewed Journal)
Ramirez-Medina, E., Vuono, E.A., Pruitt, S., Rai, A., Silva, E., Zhu, J.J., Velazquez-Salinas, L., Gladue, D.P., Borca, M.V. 2020. The C962R ORF of African swine fever strain Georgia is non-essential and not required for virulence in swine. Viruses. https://doi.org/10.3390/v12060676.
A Single Amino Acid Substitution In The Matrix Protein (M51R) Of Vesicular Stomatitis New Jersey Virus Impairs Replication In Cultured Porcine Macrophages And Results In Significant Attenuation In Pigs Reprint Icon - (Peer Reviewed Journal)
Velazquez-Salinas, L., Pauszek, S.J., Holinka, L.G., Gladue, D.P., Rekant, S.I., Bishop, E.A., Stenfeldt, C., Verdugo-Rodriguez, A., Borca, M.V., Rodriguez, L.L., Arzt, J. 2020. A single amino acid substitution in the matrix protein (M51R) of Vesicular Stomatitis New Jersey virus impairs replication in cultured porcine macrophages and results in significant attenuation in pigs. Frontiers in Microbiology. https://doi.org/10.3389/fmicb.2020.01123.
SERTA domain containing protein 1 (SERTAD1) interacts with classical swine fever virus structural glycoprotein E2 which is involved in virus virulence in swine Reprint Icon - (Peer Reviewed Journal)
Vuono, E., Ramirez-Medina, E., Azzinaro, P.A., Berggren, K., Rai, A., Pruitt, S., Silva, E., Velazquez-Salinas, L., Borca, M.V., Gladue, D.P. 2020. SERTA domain containing protein 1 (SERTAD1) interacts with classical swine fever virus structural glycoprotein E2 which is involved in virus virulence in swine. Journal of Virology. https://doi.org/10.3390/v12040421.
Detection and quantification of African swine fever in MA104 cells Reprint Icon - (Peer Reviewed Journal)
Rai, A., Pruitt, S.E., Ramierez-Medina, E., Vuono, E., Silva, E., Velazquez-Salinas, L., Carrillo, C., Gladue, D.P., Borca, M.V. 2020. Detection and quantification of African swine fever in MA104 cells. Bio-protocol. https://doi.org/10.21769/BioProtoc.3955.
Swine host protein coiled-coil domain containing 115 (CCDC115) interacts with classical swine fever virus structural glycoprotein E2 during virus Reprint Icon - (Peer Reviewed Journal)
Vuonno, E., Ramirez-Media, E., Berggren, K., Rai, A., Pruitt, S., Silva, E., Velazquez-Salinas, L., Gladue, D.P., Borca, M.V. 2020. Swine host protein coiled-coil domain containing 115 (CCDC115) interacts with classical swine fever virus structural glycoprotein E2 during virus. Viruses. https://doi.org/10.3390/v12040388.
The MGF360-16R ORF of African swine fever virus strain Georgia encodes for a non-essential gene that interacts with host proteins SERTAD3 and SDCBP Reprint Icon - (Peer Reviewed Journal)
Ramirez-Medina, E., Silva, E., Rai, A., Pruitt, S.E., Vuono, E.A., Velazquez-Salinas, L., Berggren, K.A., Zhu, J.J., Borca, M.V., Gladue, D.P. 2020. The MGF360-16R ORF of African swine fever virus strain Georgia encodes for a non-essential gene that interacts with host proteins SERTAD3 and SDCBP. Viruses. https://doi.org/10.1128/JVI.02017-19.
Deletion of CD2-like gene from the genome of African swine fever virus strain Georgia does not attenuate virulence in swine. Reprint Icon - (Abstract Only)
Borca, M.V., Ramirez Media, E., Silva, E., Vuono, E., Rai, A., Pruitt, S., Holinka, L., Velazquezsalinas, L., Gladue, D.P., Risatti, G., Shi, J. 2020. Deletion of CD2-like gene from the genome of African swine fever virus strain Georgia does not attenuate virulence in swine.. Meeting Abstract. https://doi.org/10.1038/s41598-020-57455-3.
Mechanisms of African Swine Fever Virus Pathogenesis and Immune Evasion Inferred from Gene Expression Changes in Infected Swine Macrophages - (Peer Reviewed Journal)
Structural glycoprotein E2 of classical swine fever virus binding to host protein dynactin subunit (DCTN6) is necessary for virus virulence in swine Reprint Icon - (Peer Reviewed Journal)
Borca, M.V., Vuono, E.A., Ramirez-Medina, E., Azzinaro, P.A., Berggren, K.A., Singer, M., Rai, A., Pruitt, S., Silva, E., Velazquez-Salinas, L., Carrillo, C., Gladue, D.P. 2019. Structural glycoprotein E2 of classical swine fever virus binding to host protein dynactin subunit (DCTN6) is necessary for virus virulence in swine. Journal of Virology. https://doi.org/10.1128/JVI.01642-19.
The differential effect of the deletion of African swine fever virus virulence-associated genes in the induction of attenuation of the highly virulent Georgia strain Reprint Icon - (Peer Reviewed Journal)
Ramirez-Medina, E., Vuono, E., O'Donnell, V., Holinka, L.G., Silva, E., Rai, A., Pruitt, S., Carrillo, C., Gladue, D.P., Borca, M.V. 2019. The differential effect of the deletion of African swine fever virus virulence-associated genes in the induction of attenuation of the highly virulent Georgia strain. Virology. https://doi.org/10.3390/v11070599.
The role of interleukin 6 during viral infections Reprint Icon - (Peer Reviewed Journal)
Velazquez-Salinas, L., Verdugo-Rodriguez, A., Rodriguez, L.L., Borca, M.V. 2019. The role of interleukin 6 during viral infections. Frontiers in Microbiology. https://doi.org/10.3389/fmicb.2019.01057.
Interaction of structural glycoprotein E2 of Classical Swine Fever Virus with protein phosphatase 1 catalytic subunit beta (PP1ß) - (Peer Reviewed Journal)
Vuono, E., Ramirez-Medina, E., Holinka-Patterson, L.G., Baker-Branstetter, R., Borca, M.V., Gladue, D.P. 2019. Interaction of structural glycoprotein E2 of Classical Swine Fever Virus with protein phosphatase 1 catalytic subunit beta (PP1ß). Virology. Viruses 2019, 11(4), 307. https://doi.org/10.3390/v11040307.
Validation of a site-specific recombination cloning technique for the rapid development of an infectious cDNA clone of a virulent field strain of vesicular stomatitis New Jersey virus Reprint Icon - (Peer Reviewed Journal)
Velazquez-Salinas, L., Pauszek, S.J., Barrera, J., Clark, B., Borca, M.V., Verdugo-Rodriguez, A., Arzt, J., Rodriguez, L.L. 2019. Validation of a site-specific recombination cloning technique for the rapid development of an infectious cDNA clone of a virulent field strain of vesicular stomatitis New Jersey virus. Journal of Virological Methods. Volume 265, March 2019, Pages 113-116. https://doi.org/10.1016/j.jviromet.2019.01.003.
Identification of structural glycoprotein E2 domain critical to mediate Classical Swine Fever Virus replication in SK6 cells Reprint Icon - (Peer Reviewed Journal)
Borca, M.V., Holinka-Patterson, L.G., Ramirez-Medina, E., Vuono, E., Berggren, K., Gladue, D.P. 2018. Identification of structural glycoprotein E2 domain critical to mediate Classical Swine Fever Virus replication in SK6 cells. Virology. 526:38-44. https://doi.org/10.1016/j.virol.2018.10.004.
Molecular characterization of the viroporin function of foot-and-mouth disease virus non-structureal protein 2B Reprint Icon - (Peer Reviewed Journal)
Gladue, D.P., Largo, E., De La Arada, I., Aguilella, V., Alcaraz, A., Arrondo, J., Holinka-Patterson, L.G., Brocchi, E., Ramirez-Medina, E., Vuono, E., Berggren, K., Carrillo, C., Borca, M.V. 2018. Molecular characterization of the viroporin function of foot-and-mouth disease virus non-structureal protein 2B. Journal of Virology. https://doi.org/10.1128/JVI.01360-18.
Classical swine fever virus p7 protein interacts with host protein CAMLG and regulates calcium permeability at the endoplasmic reticulum Reprint Icon - (Peer Reviewed Journal)
Gladue, D.P., Largo, E., Holinka-Patterson, L.G., Ramirez-Medina, E., Vuono, E.A., Berggren, K., Risatti, G.R., Nieva, J.L., Borca, M.V. 2018. Classical swine fever virus p7 protein interacts with host protein CAMLG and regulates calcium permeability at the endoplasmic reticulum. Virus Research. 10(9):E460. https://doi.org/10.3390/v10090460.
Characterization of naturally occurring, acute and persistent subclinical foot-and-mouth disease virus infection in vaccinated Asian buffalo in Pakistan - (Peer Reviewed Journal)
Increased virulence of an epidemic strain of vesicular stomatitis virus is associated with interference of the innate response in pigs Reprint Icon - (Peer Reviewed Journal)
Velazquez-Salinas, L., Pauszek, S.J., Stenfeldt, C., O'Hearn, E.S., Pacheco, J.M., Borca, M.V., Verdugo-Rodriguez, A., Arzt, J., Rodriguez, L.L. 2018. Increased virulence of an epidemic strain of vesicular stomatitis virus is associated with interference of the innate response in pigs. Frontiers in Microbiology. 9:1891. https://doi.org/10.3389/fmicb.2018.01891.
CRISPR/Cas gene editing of a large DNA virus: African Swine Fever Virus Reprint Icon - (Peer Reviewed Journal)
Borca, M.V., Berggren, K., Ramirez, E., Vuono, E., Gladue, D.P. 2018. CRISPR/Cas gene editing of a large DNA virus: African Swine Fever Virus. Bio-protocol. 8(16). https://doi.org/10.21769/BioProtoc.2978.
The L83L ORF of African swine fever virus strain Georgia encodes for a non-essential gene that interacts with host protein IL-1ß Reprint Icon - (Peer Reviewed Journal)
Borca, M.V., O'Donnell, V., Holinka-Patterson, L.G., Ramirez, E., Vuono, E., Berggren, K., Alfano, M., Carey, L.B., Richt, J.A., Risatti, G.R., Gladue, D.P. 2018. The L83L ORF of African swine fever virus strain Georgia encodes for a non-essential gene that interacts with host protein IL-1ß. Virus Research. 249:116-123. https://doi.org/10.1016/j.virusres.2018.03.017.
ICTV Virus taxonomy profile: Asfarviridae Reprint Icon - (Review Article)
Alonso, C., Borca, M.V., Dixon, L., Revilla, Y., Rodriguez, F., Escribano, J.M. 2018. ICTV Virus taxonomy profile: Asfarviridae. Journal of General Virology. 99:613-614. https://doi.org/10.1099/jgv.0.001049.
CRISPR-Cas9, a tool to efficiently increase the development of recombinant African swine fever viruses Reprint Icon - (Peer Reviewed Journal)
Borca, M.V., Holinka-Patterson, L.G., Berggren, K., Gladue, D.P. 2018. CRISPR-Cas9, a tool to efficiently increase the development of recombinant African swine fever viruses. Scientific Reports. 8(3154). https://doi.org/10.1038/s41598-018-21575-8.
Systemic antibodies administered by passive immunization prevent generalization of the infection by foot-and-mouth disease virus in cattle after oronasal challenge Reprint Icon - (Peer Reviewed Journal)
Barrionuevo, F., Di Giacomo, S., Bucafusco, D., Ayude, A., Schammas, J., Miraglia, M.C., Capozzo, A., Borca, M.V., Perez-Filgueira, M. 2018. Systemic antibodies administered by passive immunization prevent generalization of the infection by foot-and-mouth disease virus in cattle after oronasal challenge. Virology. 518:143-151. https://doi.org/10.1016/j.virol.2018.02.012.
A partial deletion within foot-and-mouth disease virus non-structural protein 3A causes clinical attenuation in cattle but does not prevent subclinical infection Reprint Icon - (Peer Reviewed Journal)
Stenfeldt, C., Arzt, J., Pacheco, J., Gladue, D.P., Smoliga, G.R., Silva, E., Rodriguez, L.L., Borca, M.V. 2018. A partial deletion within foot-and-mouth disease virus non-structural protein 3A causes clinical attenuation in cattle but does not prevent subclinical infection. Virology. 516:115-126. https://doi.org/10.1016/j.virol.2018.01.008.
Mutation-induced changes of transmembrane pore size revealed by combined ion-channel conductance and single vesicle permeabilization analyses Reprint Icon - (Peer Reviewed Journal)
Largo, E., Gladue, D.P., Aguilella, V.M., Alcaraz, A., Borca, M.V., Nieva, J.L. 2018. Mutation-induced changes of transmembrane pore size revealed by combined ion-channel conductance and single vesicle permeabilization analyses. Biochimica et Biophysica Acta. 1860:1015-1021. https://doi.org/10.1016/j.bbamem.2018.01.012.
Early protection events in swine immunized with an experimental live attenuated classical swine fever marker vaccine, FlagT4G - (Peer Reviewed Journal)
Holinka-Patterson, L.G., O'Donnell, V., Risatti, G., Azzinaro, P.A., Arzt, J., Stenfeldt, C., Velazquez-Salinas, L., Gladue, D.P., Borca, M.V. 2017. Early protection events in swine immunized with an experimental live attenuated classical swine fever marker vaccine, FlagT4G. PLoS One. doi: 10.1371/journal.pone.0177433.
Development of a fluorescent ASFV strain that retains the ability to cause disease in swine - (Peer Reviewed Journal)
Borca, M.V., O'Donnell, V., Holinka-Patterson, L.G., Sanford, B., Azzinaro, P.A., Risatti, G., Gladue, D.P. 2017. Development of a fluorescent ASFV strain that retains the ability to cause disease in swine. Nature Scientific Reports. 7:46747. doi: 10.1038/srep46747.
Simultaneous deletion of the 9GL and UK genes from the African swine fever virus Georgia 2007 isolate results in virus attenuation and may be a potential virus vaccine strain - (Peer Reviewed Journal)
O'Donnell, V., Risatti, G.R., Holinka-Patterson, L.G., Krug, P.W., Carlson, J., Velazquez-Salinas, L., Azzinaro, P., Gladue, D.P., Borca, M.V. 2017. Simultaneous deletion of the 9GL and UK genes from the African swine fever virus Georgia 2007 isolate results in virus attenuation and may be a potential virus vaccine strain. Journal of Virology. 91(1):e01760-16.
Association of the host immune response with protection using a live attenuated African swine fever virus model Reprint Icon - (Peer Reviewed Journal)
Carlson, J., O'Donnell, V.K., Alfano, M., Velazquez-Salinas, L., Holinka-Patterson, L.G., Krug, P.W., Gladue, D.P., Higgs, S., Borca, M.V. 2016. Association of the host immune response with protection using a live attenuated African swine fever virus model . Viruses. 8(10):E291. https://doi.org/10.3390/v8100291.
Association of the host immune response with protection using a live attenuated African swine fever virus model - (Peer Reviewed Journal)
Carlson, J., O'Donnell, V., Alsfonso, M., Velazquez Salinas, L., Holinka-Patterson, L.G., Krug, P.W., Gladue, D.P., Higgs, S., Borca, M.V. 2016. Association of the host immune response with protection using a live attenuated african swine fever virus model. Viruses. 8(10):291. doi: 10.3390/v8100291.
Alteration of a second putative fusion peptide of structural glycoprotein E2 of Classical Swine Fever Virus alters virus replication and virulence in swine - (Peer Reviewed Journal)
Holinka-Patterson, L.G., Largo, E., Gladue, D.P., O'Donnell, V.K., Risatti, G.R., Nieva, J.L., Borca, M.V. 2016. Alteration of a second putative fusion peptide of structural glycoprotein E2 of Classical Swine Fever Virus alters virus replication and virulence in swine. Journal of Virology. 90:10299-10308. doi: 10.1128/JVI.01530-16.
The Ep152R ORF of African Swine Fever Virus strain Georgia encodes for an essential gene that interacts with host protein BAG6 Reprint Icon - (Peer Reviewed Journal)
Borca, M.V., O'Donnell, V., Holinka-Patterson, L.G., Rai, D., Sanford, B., Alfano, M., Carlson, J., Azzinaro, P., Alonso, C., Gladue, D.P. 2016. The Ep152R ORF of African Swine Fever Virus strain Georgia encodes for an essential gene that interacts with host protein BAG6. Virus Research. 223:181-189. doi: 10.1016/j.virusres.2016.07.013.
African Swine Fever Virus Georgia isolate harboring deletions of 9GL and MGF360/505 genes in highly attenuated in swine but does not confer protection against parental virus challenge - (Peer Reviewed Journal)
O'Donnell, V.K., Holinka-Patterson, L.G., Gladue, D.P., Sanford, B., Krug, P.W., Carlson, J., Pacheco, J., Reese, B., Risatti, G.R., Borca, M.V. 2016. African Swine Fever Virus Georgia isolate harboring deletions of 9GL and MGF360/505 genes in highly attenuated in swine but does not confer protection against parental virus challenge. Virology. 221:8-14. doi: 10.1016/j.virusres.2016.05.014.
Recoding structural glycoprotein E2 in classical swine fever virus (CSFV) produces complete virus attenuation in swine and protects infected animals against disease - (Peer Reviewed Journal)
Velazquez-Salinas, L., Risatti, G.R., Holinka-Patterson, L.G., O'Donnell, V.K., Carlson, J., Alfano, M., Rodriguez, L.L., Carrillo, C., Gladue, D.P., Borca, M.V. 2016. Recoding classical swine fever virus (CSFV) structural glycoprotein E2 produces complete virus attenuation in swine and protects infected animals against disease. Virology. 494:178-189. doi: 10.1016/j.virol.2016.4.007.
Viral hemorrhagic fevers of animals caused by DNA viruses - (Book / Chapter)
Borca, M.V., Gay, C.G., Risatti, G.R., O'Toole, D., Li, N., Kuhn, J.H., Lewis, C.E., Loiacono, C.M., White, D. 2016. Viral hemorrhagic fevers of animals caused by DNA viruses. Book Chapter. Borca. 2015. Global Virology I: Identifying and Invetsigating Viral Diseases. Springer Science, NY.
Viral hemorrhagic fevers of animals caused by positive-stranded RNA viruses - (Book / Chapter)
Borca, M.V., Van Campen, H., Risatti, G.R., Kerr, P., Strive, T., Jhaling, P.B., Kuhn, J.H., Lewis, C.E., Loiacono, C.M., White, D. 2016. Viral hemorrhagic fevers of animals caused by positive-stranded RNA viruses. Book Chapter. Borca, M. V. Global Virology I: Springer Science, NY P 361-401.
Overview of Classical Swine Fever (Hog Cholera, Classical Swine fever) - (Book / Chapter)
Risatti, G.R., Borca, M.V. 2016. Overview of Classical Swine Fever (Hog Cholera, Classical Swine fever) . Book Chapter. Borca, M.V. The Merck Veterinary Manual. The Merck Publishing Group 2016.
Deletion of the thymidine kinase gene induces complete attenuation of the Georgia isolate of African swine fever virus - (Peer Reviewed Journal)
Sandford, B., Holinka-Patterson, L.G., O'Donnell, V., Krug, P.W., Carlson, J., Alfano, M., Carrillo, C., Wu, P., Lowe, A., Risatti, G.R., Gladue, D.P., Borca, M.V. 2015. Deletion of the thymidine kinase gene induces complete attenuation of the Georgia isolate of African swine fever virus. Virus Research. 213:165-171. doi: 10.1016/j.viruses.2015.12.002.
Systemic foot-and-mouth disease vaccination in cattle promotes specific antibody secreting cells at the respiratory tract and triggers local anamnestic-compatible responses upon aerosol infection Reprint Icon - (Peer Reviewed Journal)
Pega, J., Di Giacomo, S., Bucafusco, D., Schammas, J.M., Barrionuevo, F., Capozzo, A., Rodriguez, L.L., Borca, M.V., Perez-Filgueira, M. 2015. Systemic foot-and-mouth disease vaccination in cattle promotes specific antibody secreting cells at the respiratory tract and triggers local anamnestic-compatible responses upon aerosol infection. Journal of Virology. 89(18):9581-9590. doi: 10.1128/JVI.01082-15.
Deletion of African swine fever virus Georgia 2007 virulence-associated gene 9GL (B119L) leads to virus attenuation in swine at low doses while inducing an effective protection against homologous challenge - (Peer Reviewed Journal)
O'Donnell, V., Holinka-Patterson, L.G., Krug, P.W., Gladue, D.P., Carlson, J., Sanford, B.J., Alfano, M., Kramer, E., Lu, Z., Arzt, J., Reese, B., Carrillo, C., Risatti, G., Borca, M.V. 2015. Deletion of African swine fever virus Georgia 2007 virulence-associated gene 9GL (B119L) leads to virus attenuation in swine at low doses while inducing an effective protection against homologous challenge. Journal of Virology. 89(16):8556-8566. DOI: 10.1128/JVI.00969-15.
Treatment with interferon-alpha delays disease in swine infected with a highly virulent CSFV strain Reprint Icon - (Peer Reviewed Journal)
Fernandez-Sainz, I., Zhu, J.J., O'Donnell, V., Diaz San Segundo, F.C., Velazquez, L., De Los Santos, T.B., Borca, M.V. 2015. Treatment with interferon-alpha delays disease in swine infected with a highly virulent CSFV strain. Virology. 483:284-290. DOI: 10.1016/j.virol.2015.04.024.
Viral hemorrhagic fevers of animals caused by DNA viruses - (Book / Chapter)
Borca, M.V., Gay, C.G., Risatti, G., O'Toole, G., Li, H., Kuhn, J., Lewis, C., Loiacono, C., White, D.M. 2015. Viral hemorrhagic fevers of animals caused by DNA viruses. In:Shapshak, P., Sinnott, J.T., Somboonwit, C., Kuhn, J., editors. Global Virology-Identifying and Investigating Viral Diseases. New York, NY:Springer. p. 319-343.
African swine fever virus Georgia isolate harboring deletions of MGF360 and MGF505 genes is attenuated in swine and confers protection against challenge with the virulent parental virus Reprint Icon - (Peer Reviewed Journal)
O'Donnell, V., Holinka-Patterson, L.G., Gladue, D.P., Sanford, B.J., Krug, P.W., Lu, Z., Arzt, J., Reese, B., Carrillo, C., Risatti, G.R., Borca, M.V. 2015. African swine fever virus Georgia isolate harboring deletions of MGF360 and MGF505 genes is attenuated in swine and confers protection against challenge with the virulent parental virus. Journal of Virology. 89(11):6048-6056.
Progressive adaptation of a Georgian isolate of African swine fever virus to vero cells leads to a gradual attenuation of virulence in swine corresponding to major changes of the viral genome Reprint Icon - (Peer Reviewed Journal)
Krug, P.W., Holinka-Patterson, L.G., O'Donnell, V., Reese, B., Sanford, B.J., Fernandez-Sainz, I., Gladue, D.P., Arzt, J., Rodriguez, L.L., Risatti, G.R., Borca, M.V. 2014. Progressive adaptation of a Georgian isolate of African swine fever virus to vero cells leads to a gradual attenuation of virulence in swine corresponding to major changes of the viral genome. Journal of Virology. 89(4):2324-2332.
Virus-host interactions in persistently FMDV-infected cells derived from bovine pharynx - (Peer Reviewed Journal)
O'Donnell, V., Pacheco Tobin, J., Larocco, M.A., Gladue, D.P., Pauszek, S.J., Smoliga, G.R., Krug, P.W., Baxt, B., Borca, M.V., Rodriguez, L.L. 2014. Virus-host interactions in persistently FMDV-infected cells derived from bovine pharynx. Virology. 468-470C:185-196.
Morphologic and phenotypic characteristics of myocarditis in two pigs infected by foot-and mouth disease virus strains of serotypes O or A - (Peer Reviewed Journal)
Stenfeldt, C., Pacheco Tobin, J., Borca, M.V., Rodriguez, L.L., Arzt, J. 2014. Morphologic and phenotypic characteristics of myocarditis in two pigs infected by foot-and mouth disease virus strains of serotypes O or A. Acta Veterinaria Scandinavica. 56(1):42.
Interaction of structural core protein of Classical Swine Fever Virus with endoplasmic reticulum-associated degradation pathway protein OS9 - (Peer Reviewed Journal)
Gladue, D.P., O'Donnell, V., Fernandez-Sainz, I.J., Fletcher, P., Baker-Branstetter, R., Holinka-Patterson, L.G., Sanford, B.J., Carlson, J., Lu, Z., Borca, M.V. 2014. Interaction of structural core protein of Classical Swine Fever Virus with endoplasmic reticulum-associated degradation pathway protein OS9. Virology. 460-461:173-179. DOI: 10.1016/j.virol.2014.05.008.
Effect of specific amino acid substitutions in the putative fusion peptide of structural glycoprotein E2 on Classical Swine Fever Virus replication - (Peer Reviewed Journal)
Fernandez-Sainz, I.J., Largo, E., Gladue, D.P., Fletcher, P., O'Donnell, V., Holinka-Patterson, L.G., Carey, B., Lu, Z., Nieva, J.L., Borca, M.V. 2014. Effect of specific amino acid substitutions in the putative fusion peptide of structural glycoprotein E2 on Classical Swine Fever Virus replication. Virology. 456-457:121-130.
Interaction of foot-and-mouth disease virus non-structural protein 3A with host protein DCTN3 is important for viral virulence in cattle - (Peer Reviewed Journal)
Gladue, D.P., O'Donnell, V., Baker-Branstetter, R., Pacheco Tobin, J., Holinka-Patterson, L.G., Arzt, J., Pauszek, S.J., Fernandez-Sainz, I., Fletcher, P., Brocchi, E., Lu, Z., Rodriguez, L.L., Borca, M.V. 2014. Interaction of foot-and-mouth disease virus non-structural protein 3A with host protein DCTN3 is important for viral virulence in cattle. Journal of Virology. 88(5):2737-2747.
Interaction of CSFV E2 protein with swine host factors as detected by yeast two-hybrid system - (Peer Reviewed Journal)
Gladue, D.P., Baker-Branstetter, R., Holinka-Patterson, L.G., Fernandez-Sainz, I.J., O'Donnell, V., Fletcher, P., Lu, Z., Borca, M.V. 2014. Interaction of CSFV E2 protein with swine host factors as detected by yeast two-hybrid system . PLoS One. 9(1):e68532.
Pore-forming activity of pestivirus p7 in a minimal model system supports genus-specific viroporin function - (Peer Reviewed Journal)
Largo, E., Gladue, D.P., Huarte, N., Borca, M.V., Nieva, J.L. 2013. Pore-forming activity of pestivirus p7 in a minimal model system supports genus-specific viroporin function. Antiviral Research. 101:30-36.
The pathogenesis of highly virulent African Swine Fever virus in domestic pigs exposed via intraoropharyngeal, intranasopharyngeal, and intramuscular inoculation, and by direct contact with infected pigs Reprint Icon - (Peer Reviewed Journal)
Howey, E.B., O'Donnell, V., De Carvalho Ferreira, H.C., Borca, M.V., Arzt, J. 2013. The pathogenesis of highly virulent African Swine Fever virus in domestic pigs exposed via intraoropharyngeal, intranasopharyngeal, and intramuscular inoculation, and by direct contact with infected pigs. Virus Research. 178(2):328-339.
A partial deletion in non-structural protein 3A can attenuate foot-and-mouth disease virus in cattle Reprint Icon - (Peer Reviewed Journal)
Pacheco Tobin, J., Gladue, D.P., Holinka-Patterson, L.G., Arzt, J., Bishop, E.A., Smoliga, G.R., Pauszek, S.J., Bracht, A.J., O'Donnell, V., Fernandez-Sainz, I., Fletcher, P., Piccone, M.E., Rodriguez, L.L., Borca, M.V. 2013. A partial deletion in non-structural protein 3A can attenuate foot-and-mouth disease virus in cattle. Virology. 446(1-2):260-267.
Heterogeneity in the antibody response to foot-and-mouth disease primo-vaccinated calves - (Peer Reviewed Journal)
Di Giacomo, S., Brito, B.P., Perez, A.M., Bucafusco, D., Pega, J., Rodriguez, L.L., Borca, M.V., Perez-Filgueira, M. 2013. Heterogeneity in the antibody response to foot-and-mouth disease primo-vaccinated calves. Transboundary and Emerging Diseases. DOI:10.1111/tbed.12130.
Foot-and-mouth disease virus modulates cellular vimentin for virus survival Reprint Icon - (Peer Reviewed Journal)
Gladue, D.P., O'Donnell, V., Baker-Branstetter, R., Holinka-Patterson, L.G., Pacheco Tobin, J., Fernandez Sainz, I.J., Lu, Z., Brocchi, E., Piccone, M.E., Fletcher, P., Ambroggio, X., Rodriguez, L.L., Borca, M.V. 2013. Foot-and-mouth disease virus modulates cellular vimentin for virus survival. Journal of Virology. 87(12):6794-6803.
Early adaptive immune responses in the respiratory tract of foot and mouth disease-infected cattle - (Peer Reviewed Journal)
Pega, J., Bucafusco, D., Di Giacomo, S., Malacari, D., Capozzo, A., Arzt, J., Perez-Beascoechea, C., Maradei, E., Rodriguez, L.L., Borca, M.V., Perez-Filgueira, M. 2012. Early adaptive immune responses in the respiratory tract of foot and mouth disease-infected cattle. Journal of Virology. 87(5):2489-2495.
Foot and mouth disease virus non structural protein 2C interacts with Beclin1 modulating virus replication - (Peer Reviewed Journal)
Gladue, D.P., O'Donnell, V., Baker-Branstetter, R., Pacheco Tobin, J., Holinka-Patterson, L.G., Fernandez Sainz, I.J., Lu, Z., Brocchi, E., Baxt, B., Piconne, M.E., Rodriguez, L.L., Borca, M.V. 2012. Foot and mouth disease virus non structural protein 2C interacts with Beclin1 modulating virus replication. Journal of Virology. 86(22):12080-12090. doi:10.1128/JVI.01610-12.
Classical Swine Fever Virus p7 protein is a viroporin involved in virulence in swine - (Peer Reviewed Journal)
Gladue, D.P., Holinka-Patterson, L.G., Largo, E., Fernandez Sainz, I.J., O'Donnell, V., Baker-Branstetter, R., Lu, Z., Ambroggio, X., Risatti, G.R., Nieva, J.L., Borca, M.V. 2012. Classical Swine Fever Virus p7 protein is a viroporin involved in virulence in swine. Journal of Virology. 86(12):6778-6791.
Role of the Arg56 of the structural protein VP3 of foot-and-mouth disease virus (FMDV) 01 campos in virus virulence - (Peer Reviewed Journal)
Borca, M.V., Pacheco Tobin, J., Holinka-Patterson, L.G., Hartwig, E.J., Carrillo, C., Garriga, D., Kramer, E., Rodriguez, L.L., Piccone, M.E. 2012. Role of the Arg56 of the structural protein VP3 of foot-and-mouth disease virus (FMDV) 01 campos in virus virulence. Virology. (422):37-45.
Effects of glycosylation on antigenicity and immunogenicity of classical swine fever virus envelope proteins - (Peer Reviewed Journal)
Gavrilov, B.K., Rogers, K., Fernandez Sainz, I.J., Holinka-Patterson, L.G., Borca, M.V., Risatti, G.R. 2011. Effects of glycosylation on antigenicity and immunogenicity of classical swine fever virus envelope proteins. Virology. 420:135-145.
Interaction between core protein of classical swine fever virus with cellular IQGAP1 proetin appears essential for virulence in swine - (Peer Reviewed Journal)
Gladue, D.P., Holinka-Patterson, L.G., Fernandez-Sainz, I., Prarat, M.V., O'Donnell, V.K., Vepkhvadze, N., Lu, Z., Risatti, G.R., Borca, M.V. 2011. Interaction between core protein of classical swine fever virus with cellular IQGAP1 proetin appears essential for virulence in swine. Virology. 412:68-74.
Identification of an NTPase motif in classical swine fever virus NS4B protein - (Peer Reviewed Journal)
Gladue, D.P., Gavrilov, B.K., Holinka-Patterson, L.G., Fernandez-Sainz, I.J., Vepkhvadze, N.G., Rogers, K., O'Donnell, V., Borca, M.V. 2011. Identification of an NTPase motif in classical swine fever virus NS4B protein. Virology. 411(1):41-49.
Foot-and-mouth disease virus utilizes an autophagic pathway during viral replication - (Peer Reviewed Journal)
O'Donnell, V., Pacheco Tobin, J., Larocco, M.A., Burrage, T., Jackson, W., Rodriguez, L.L., Borca, M.V., Baxt, B. 2010. Foot-and-mouth disease virus utilizes an autophagic pathway during viral replication. Virology. 410:142-150.
Substitution of specific cysteine residues in E1 glycoprotein of classical swine fever virus strain Brescia affects formation of E1-E2 heterodimers and alters virulence in swine - (Peer Reviewed Journal)
Fernandez-Sainz, I.J., Holinka-Patterson, L.G., Gladue, D.P., O'Donnell, V., Lu, Z., Gavrilov, B.K., Risatti, G.R., Borca, M.V. 2011. Substitution of specific cysteine residues in E1 glycoprotein of classical swine fever virus strain Brescia affects formation of E1-E2 heterodimers and alters virulence in swine. Journal of Virology. 407:129-136.
Effects of the interactions of classical swine fever virus core protein with proteins of SUMOylation pathway on virulence in swine - (Peer Reviewed Journal)
Gladue, D.P., Holinka-Patterson, L.G., Fernandez-Saniz, I., Prarat, M.V., O'Donnell, V.K., Vepkhvadze, N., Lu, Z., Rogers, K., Risatti, G.R., Borca, M.V. 2010. Effects of the interactions of classical swine fever virus core protein with proteins of SUMOylation pathway on virulence in swine. Virology. 407:129-136.
Domain disruptions of individual 3B proteins for foot-and-mouth disease virus do not alter growth in cell culture nor virulence in cattle - (Peer Reviewed Journal)
Pacheco Tobin, J., Piccone, M.E., Rieder, A.E., Pauszek, S.J., Borca, M.V., Rodriguez, L.L. 2010. Domain disruptions of individual 3B proteins for foot-and-mouth disease virus do not alter growth in cell culture nor virulence in cattle. Virology. DOI: 10.1016/j.virol.2010.05.036.
Mutations in the classical swine fever virus NS4B protein affects virulence in swine - (Abstract Only)
Fernandez Sainz, I., Gladue, D.P., Holinka-Patterson, L.G., O'Donnell, V.K., Gudmundsdottir, I., Prarat, M.V., Patch, J.R., Golde, W.T., Zhiqiang, L., Risatti, G.R., Zhu, J.J., Borca, M.V. 2009. Mutations in the classical swine fever virus NS4B protein affects virulence in swine. Proceedings. P. 143.
Mutations in classical swine fever virus NS4B affect virulence in swine - (Peer Reviewed Journal)
Fernandez-Sainz, I., Gladue, D.P., Holinka-Patterson, L.G., O'Donnell, V.K., Gudmundsdottir, I., Prarat, M.V., Patch, J.R., Golde, W.T., Lu, Z., Risatti, G.R., Zhu, J.J., Borca, M.V. 2010. Mutations in classical swine fever virus NS4B affect virulence in swine. Journal of Virology. 84(3):1536-1549.
The region between the two polyprotein initiation codons of foot-and-mouth disease virus is critical for virulence in cattle - (Peer Reviewed Journal)
Piccone, M.E., Pacheco Tobin, J., Pauszek, S.J., Borca, M.V., Rieder, A.E., Kramer Jr, E.V., Zhu, J.J., Rodriguez, L.L. 2010. The region between the two polyprotein initiation codons of foot-and-mouth disease virus is critical for virulence in cattle. Virology. 396:152-159.
Sumoylation of the Core Protein in Classical Swine Fever Virus is Essential for Virulence in swine - (Abstract Only)
Gladue, D.P., Holinka-Patterson, L.G., Fernandez-Sainz, I., Prarat, M.V., Lu, Z., Borca, M.V. 2009. Sumoylation of the Core Protein in Classical Swine Fever Virus is Essential for Virulence in swine. American Society for Virology Meeting. Paper No. W49-5: P. 213.
Alteration of the N-linked Glycosylation Condition of E1 Glycoprotein of Classical Swine Fever Virus Strain Brescia Alters Virulence in Swine - (Peer Reviewed Journal)
Fernandez-Sainz, I.J., Holinka-Patterson, L.G., Gavrilov, B.K., Prarat, M.V., Gladue, D.P., Lu, Z., Jia, W., Risatti, G.R., Borca, M.V. 2009. Alteration of the N-linked Glycosylation Condition of E1 Glycoprotein of Classical Swine Fever Virus Strain Brescia Alters Virulence in Swine. Virology. 386:210-216.
Development of a live attenuated antigenic marker classical swine fever vaccine - (Peer Reviewed Journal)
Holinka-Patterson, L.G., Fernandez-Sainz, I., O'Donnell, V., Prarat, M.V., Gladue, D.P., Lu, Z., Risatti, G., Borca, M.V. 2008. Development of a live attenuated antigenic marker classical swine fever vaccine. Virology. 384:106-113.
Patterns of gene expression in swine macrophages infected with classical swine fever virus detected by microarray - (Peer Reviewed Journal)
Gladue, D.P., Zhu, J.J., Holinka-Patterson, L.G., Fernandez-Sainz, I., Prarat, M.V., O'Donnell, V.K., Borca, M.V. 2010. Patterns of gene expression in swine macrophages infected with classical swine fever virus detected by microarray. Virology. 138(1-2):89-96.
Patterns of Cellular Gene Expression in Swine Macrophages Infected with Highly Virulent Classical Swine Fever Virus Strain Brescia - (Peer Reviewed Journal)
Borca, M.V., Gudmundsdottir, I., Fernandez-Sainz, I.J., Holinka-Patterson, L.G., Risatti, G.R. 2008. Patterns of Cellular Gene Expression in Swine Macrophages Infected with Highly Virulent Classical Swine Fever Virus Strain Brescia. Virus Research (138): 89-96
Role of the Region Located Between the Two AUG Initiation Codons in the Pathogenesis of Foot-and-Mouth Disease Virus (FMDV) - (Abstract Only)
Piccone, M.E., Pacheco Tobin, J., Pauszek, S.J., Borca, M.V., Rieder, A.E., Rodriguez, L.L. 2008. Role of the Region Located Between the Two AUG Initiation Codons in the Pathogenesis of Foot-and-Mouth Disease Virus (FMDV). European Study Group on the Molecular Biology of Picornaviruses. p. 199.
Removal of a N-linked Glycosylation Site on the Classical Swine Fever Virus Strain Brescia E(rns) Glycoprotein Affects Virulence in Swine - (Peer Reviewed Journal)
Fernandez-Sainz, I., Holinka, L.G., Lu, Z., Risatti, G.R., Borca, M.V. 2007. Removal of a N-linked Glycosylation Site on the Classical Swine Fever Virus Strain Brescia E(rns) Glycoprotein Affects Virulence in Swine. Virology. 370:122-129.
Sheeppox virus kelch-like gene SPPV-019 affects virus virulence - (Peer Reviewed Journal)
Balinsky, C.A., Delhon, G., Afonso, C.L., Risatti, G.R., Borca, M.V., French, R.A., Tulman, E.R., Geary, S.J., Rock, D.L. 2007. Sheeppox virus kelch-like gene SPPV-019 affects virus virulence. Journal of Virology. 81(20):11392-11401.
Genetic and Phenotypic Variation of FMDV During Serial Passages in a Natural Host - (Peer Reviewed Journal)
Carrillo, C., Lu, Z., Borca, M.V., Vagnozzi, A., Kutish, G.F., Rock, D.L. 2007. Genetic and Phenotypic Variation of FMDV During Serial Passages in a Natural Host. Journal of Virology. 81(20):11341-11351. DOI: 10-1128/jvi.00930-07.
Classical Swine Fever Virus Inhibits Nitric Oxide Production in Infected Macrophages - (Peer Reviewed Journal)
Zaffuto, K.M., Piccone, M.E., Burrage, T.G., Risatti, G.R., Borca, M.V., Holinka, L.G., Rock, D.L., Afonso, C.L. 2007. Classical Swine Fever Virus Inhibits Nitric Oxide Production in Infected Macrophages. Journal of General Virology. 88:3007-3012.
Mutations in the Carboxyl Terminal Region of E2 Glycoprotein of Classical Swine Fever Virus are Responsible for Viral Attenuation in Swine - (Abstract Only)
Risatti, G.R., Holinka, L.G., Fernanez-Sainz, I., Carrillo, C., Kutish, G., Lu, Z., Zhu, J., Rock, D.L., Borca, M.V., 2007, Mutations in the Carboxyl Terminal Region of E2 Glycoprotein of Classical Swine Fever Virus are Responsible for Viral Attenuation in Swine. Positive Strand RNA Virus International Conference Proceedings. P2-K27, p.116
N-linked Glycosylation of Classical Swine Fever Virus Strain Brescia Erns Glycoprotein Alters Virulence in Swine - (Abstract Only)
Fernandez-Sainz, I., Holinka, L.G., Lu, Z., Risatti, G.R., Borca, M.V. 2007. N-linked Glycosylation of Classical Swine Fever Virus Strain Brescia Erns Glycoprotein Alters Virulence in Swine. Eighth International Symposium on Positive Strand RNA Virus, P2-K26, P.116
Mutations in the Carboxyl Terminal Region of E2 Glycoprotein of Classical Swine Fever Virus is Responsible for Viral Attenuation in Swine - (Peer Reviewed Journal)
Risatti, G., Holinka, L.G., Fernandez-Sainz, I., Carrillo, C., Kutish, G.F., Lu, Z., Zhu, J., Rock, D.L., Borca, M.V. 2007. Mutations in the Carboxyl Terminal Region of E2 Glycoprotein of Classical Swine Fever Virus is Responsible for Viral Attenuation in Swine. Virology. 364 (2): 371-82.
N-Linked Glycosylation Status Of Classical Swine Fever Virus Strain Brescia E2 Glycoprotein Influences Virulence In Swine - (Abstract Only)
Risatti, G., Holinka, L.G., Fernandez-Sainz, I., Carrillo, C., Lu, Z., Borca, M.V. 2006. N-linked glycosylation status of classical swine fever virus strain brescia e2 glycoprotein influences virulence in swine. Conference of Research Workers in Animal Disease (CRWAD) annual meeting, Chicago, IL, December 3-5, 2006, page 200.
Mutations in the Carboxi Terminal Region of E2 Glycoprotein of Classical Swine Fever Virus is Responsible for Viral Attenuation in Swine - (Abstract Only)
Risatti, G.R., Holinka, L.G., Fernandez-Sainz, I., Carillo, C., Kutish, G.F., Lu, Z., Tulman, E.R., Rock, D.L., Borca, M.V. Mutations in the Carboxi Terminal Region of E2 Glycoprotein of Classical Swine Fever Virus is Responsible for Viral Attenuation in Swine. Conference of Research Workers in Animal Disease (CRWAD) annual meeting, Chicago, IL, December 3-5, 2006, page 138.
Identification of a Novel Virulence Determinant Within the E2 Structural Glycoprotein of Classical Swine Fever Virus - (Abstract Only)
Risatti, G.R., Holinka, L.G., Carillo, C., Kutish, G.F., Lu, Z., Tulman, E.R., Fernandez-Sainz, I., Borca, M.V. 2006. Identification of a Novel Virulence Determinant Within the E2 Structural Glycoprotein of Classical Swine Fever Virus. Conference of Research Workers in Animal Disease (CRWAD) annual meeting, Chicago, IL, December 3-5, 2006, page 201.
N-LINKED GLYCOSYLATION STATUS OF CLASSICAL SWINE FEVER VIRUS STRAIN BRECIA E2 GLYCOPROTEIN INFLUENCES VIRULENCE IN SWINE - (Peer Reviewed Journal)
Rissatti, G., Holinka, L.G., Fernandez-Sainz, I., Carrillo, C., Lu, Z., Borca, M.V. 2007. N-linked glycosylation status of classical swine fever virus strain brecia e2 glycoprotein influences virulence in swine. Journal of Virology. Vol. 81, No.2, P. 924-933.
IDENTIFICATION OF A NOVEL VIRULENCE DETERMINAT WITHIN THE E2 STRUCTURAL GLYCOPROTEIN OF CLASSICAL SWINE FEVER - (Peer Reviewed Journal)
Risatti, G., Holinka, L.G., Carrillo, C., Kutish, G.F., Lu, Z., Fernandez-Sainz, I., Borca, M.V. 2006. Identification of a novel virulence determinat within the e2 structural glycoprotein of classical swine fever. Virology, 11/10/2006, Vol 355, Issue i, pages 94-101.
MUTATION OF E1 GLYCOPROTEIN OF CLASSICAL SWINE FEVER VIRUS AFFECTS VIRAL VIRULENCE IN SWINE - (Peer Reviewed Journal)
Risatti, G.R., Holinka, L.G., Lu, Z., Kutish, G.F., Tulman, E.R., French, R.A., Sur, J.H., Rock, D.L., Borca, M.V. 2005 Mutation of E1 Glycoprotein of Classical Swine Fever Virus Affects Viral Virulence in Swine. Journal of Virology 343: 116-127.
CHIMERIC CLASSICAL SWINE FEVER VIRUS CONTAINING THE E2 GENE FROM A VACCINE STRAIN IN THE GENETIC BACKGROUND OF STRAIN BRESCIA INDUCES SOLID EARLY PROTECTION IN SWINE - (Abstract Only)
Risatti, G.R., Holinka, L.G., Lu, Z., Kutish, G.F., Rock, D.L., Borca, M.V. 2005. Chimeric Classical Swine Fever Virus Containing the E2 Gene from a Vaccine Strain in the Genetic Background of Strain Brescia Induces Solid Early Protection in Swine. 6th Pestivirus Symposium, Thun, Switzerland.Page 89, P5-10
GENETIC AND PHENOTYPIC VARIATION OF FMDV DURING SERIAL PASSAGES IN PIGS - (Abstract Only)
Carrillo, C., Lu, Z., Tulman, E., Borca, M.V., Vagnozzi, A., Kutish, G.F., Rock, D.L. 2005. Genetic and Phenotypic Variation of FMDV During Serial Passages in Pigs. European Study Group on the Molecular Biology of Picornaviruses. P. E06.
NOVEL TOOLS TO PREVENT AND ERADICATE FOREIGN ANIMAL DISEASES - (Abstract Only)
Rodriguez, L.L., Baxt, B., Rieder, A.E., Golde, W.T., Borca, M.V., Grubman, M.J. 2005. Novel tools to prevent and eradicate foreign animal diseases. American Society for Microbiology Biodefense Meeting. p 41.
PRECLINICAL DIAGNOSIS OF AFRICAN SWINE FEVER IN CONTACT EXPOSED SWINE BY A PORTABLE REAL-TIME PCR ASSAY - (Proceedings)
Zsak, L., Borca, M.V., Risatti, G.R., French, R.A., Lu, Z., Kutish, G.F., Neilan, J.G., Callahan, J.D., Nelson, W.M., Rock, D.L. 2004. Preclinical diagnosis of african swine fever in contact exposed swine by a portable real-time pcr assay [abstract]. Conference Research Workers Disease Meeting. p. 87.
THE E2 GLYCOPROTEIN OF CLASSICAL SWINE FEVER VIRUS AFFECTS VIRAL VIRULENCE IN SWINE - (Peer Reviewed Journal)
Risatti, G.R., Borca, M.V., Kutish, G.F., Lu, Z., Holinka, L.G., French, R., Tulman, E., Rock, D.L. 2005 The E2 Glycoprotein of Classical Swine Fever Virus Affects Viral Virulence in Swine. Journal of Virology.79: 3787-3796.
A 19MER PEPTIDE INSERTION IN THE E1 GLYCOPROTEIN OF CLASSICAL SWINE FEVER FEVER VIRUS AFFECTS VIRAL VIRULENCE IN SWINE - (Proceedings)
Risatti, G.R., Kutish, G.F., Lu, Z., Holinka, L.G., French, R.A., Sur, J.H., Rock, D.L., Borca, M.V. 2004. A 19mer Peptide Insertion in the E1 Glycoprotein of Classical Swine Fever Virus Affects Viral Virulence in Swine. Conference of Research Worksers in Animal Disese Meeting. P.195
THE E2 GLYCOPROTEIN OF CLASSICAL SWINE FEVER VIRUS IS A VIRULENCE DETERMINANT IN SWINE - (Proceedings)
Risatti, G.R., Kutish, G.F., Lu, Z., Holinka, L.G., French, R.A., Tulman, E., Rock, D.L., Borca, M.V. 2004. The E2 Glycoprotein of Classical Swine Fever Virus is a Virulence Determinant in Swine. Conference of Research Worksers in Animal Disese Meeting. P. 196.
DIAGNOSTIC EVALUATION OF A PORTABLE REAL-TIME REVERSE TRANSCRIPTASE PCR ASSAY FOR THE DETECTION OF CLASSICAL SWINE FEVER. - (Proceedings)
Risatti, G.R., Holinka, L.G., Lu, Z., Kutish, G.F., Callahan, J.D., Nelson, W.N., Tio, B.E., Borca, M.V. 2004. Diagnostic Evaluation of a Portable Real-Time Reverse Transcriptase PCR Assay for the Detection of Classical Swine Fever. Conference of Research Worksers in Animal Disese Meeting. P 194.
DIAGNOSTIC EVALUATION OF A PORTABLE REAL-TIME REVERSE TRANSCRIPTASE PCR ASSAY FOR THE DETECTION OF CLASSICAL SWINE FEVER - (Peer Reviewed Journal)
Risatti, G.R., Holinka, L.G., Lu, Z., Kutish, G.F., Callahan, J.D., Nelson, W.M., Brea, T.E., Borca, M.V. 2005. Diagnostic Evaluation of a Portable Real-Time Reverse transcriptase PCR Assay for the Detection of Classical Swine Fever. Journal of Clinical Microbiology. 43 (1): 468-471.
PRECLINICAL DIAGNOSIS OF AFRICAN SWINE FEVER IN CONTACT EXPOSED SWINE BY A PORTABLE REAL-TIME PCR ASSAY - (Peer Reviewed Journal)
PRECLINICAL DIAGNOSIS OF AFRICAN SWINE FEVER IN CONTACT EXPOSED SWINE BY A PORTABLE REAL-TIME PCR ASSAY - (Peer Reviewed Journal)
Zsak, L., Borca, M.V., Risatti, G.R., French, R., Lu, Z., Kutish, G.F., Neilan, J.G., Callahan, J.D., Nelson, W.M., Rock, D.L. 2005. Preclinical Diagnosis of African Swine Fever in Contact Exposed Swine by a Portable Real-time PCR Assay. Journal of Clinical Microbiology. 43 (1):112-119.
DEVELOPMENT OF TRANSGENIC ALFALFA PLANTS CONTAINING THE FOOT AND MOUTH DISEASE VIRUS STRUCTURAL POLYPROTEIN GENE P1 AND ITS UTILIZATION AS AN EXPERIMENTAL IMMUNOGEN - (Peer Reviewed Journal)
Dus Santos, M., Carrillo, C., Ardila, F., Rios, R., Franzone, M., Piccone, M.E., Wigdorovitz, A., Borca, M.V. 2005. Development of Transgenic Alfalfa Plants Containing the Foot-and-Mouth Disease Virus Structural Polyprotein Gene p1 and Its Utilization as an Experimental Immunogen. Vaccine.23(15): 1838-43.
PRESERVED ANTIGENICITY OF HIV-1 P24 PRODUCED AND PURIFIED IN HIGH YIELDS FROM PLANTS INOCULATED WITH TOBACCO MOSAIC VIRUS (TMV)-DERIVED VECTOR - (Peer Reviewed Journal)
Perez, D., Brayfield, B., Phiri, S., Borca, M.V., Woods, C., Morris, T. 2004. Preserved antigenicity of hiv-1 p24 produced and purified in high yields from plants inoculated with tobacco mosaic virus (tmv)-derived vector.. Journal of Virological Methods. 121 (2004) 201-208.
PASSIVE PROTECTION TO BOVINE ROTAVIRUS (BRV) INFECTION INDUCED BY A BRV VP8* PRODUCED IN PLANTS USING A TMV-BASED VECTOR - (Peer Reviewed Journal)
Perez Filgueira, D., Mozgovoj, M., Wigdorovitz, A., Dus Santos, M., Parreno, V., Trono, K., Fernandez, F., Carrillo, C., Badiuk, L., Morris, T., Borca, M.V. 2004. Passive protection to bovine rotavirus (brv) infection induced by a brv vp8* produced in plants using a tmv-based vector.. Archives of Virology. 149:2337-2348.
A 19MER PEPTIDE INSERTION IN THE E1 GLYCOPROTEIN OF CLASSICAL SWINE FEVER VIRUS AFFECTS VIRAL VIRULENCE IN SWINE - (Proceedings)
Borca, M.V., Risatti, G.R., Kutish, G.F., Lu, Z., Holinka, L.G., French, R.A., Sur, J., Rock, D.L. 2004. A 19mer peptide insertion in the e1 glycoprotein of classical swine fever virus affects viral virulence in swine [abstract]. Positive Strand RNA Virus International Conference Proceedings. p. 109.
THE E2 GLYCOPROTEIN OF CLASSICAL SWINE FEVER VIRUS AFFECTS VIRAL VIRULENCE IN SWINE - (Proceedings)
Risatti, G.R., Kutish, G.F., Lu, Z., Holinka, L.G., French, R.A., Borca, M.V., Rock, D.L. 2004. The e2 glycoprotein of classical swine fever virus affects viral virulence in swine [abstract]. Positive Strand RNA Virus International Conference Proceedings. P. 109.
PROTECTIVE LACTOGENIC IMMUNITY CONFERRED BY AN EDIBLE PEPTIDE VACCINE TO BOVINE ROTAVIRUS PRODUCED IN TRANSGENIC PLANTS - (Peer Reviewed Journal)
Wigdorovitz, A., Mozgovoj, M., Dus Santos, M.J., Parreno, V., Perez-Filgueira, D.M., Trono, K.G., Rios, R.D., Carrillo, C., Escribano, J.M., Borca, M.V. 2004. Protective lactogenic immunity conferred by an edible peptide vaccine to bovine rotavirus produced in transgenic plants. Journal of General Virology. 85:1825-1832.
BOVINE HERPES VIRUS G-D PROTEIN PRODUCED IN PLANTS USING A RECOMBINANT TOBACCO MOSAIC VIRUS (TMV) VECTOR POSSESSES AUTHENTIC ANTIGENICITY - (Peer Reviewed Journal)
Perez Filgueira, D.M., Zamorano, P.I., Dominguez, M.G., Taboga, O., Del Medico Zajac, M.P., Puntel, M., Romera, S.A., Morris, T.J., Borca, M.V., Sadir, A.M. 2003. Bovine herpes virus g-d protein produced in plants using a recombinant tobacco mosaic virus (tmv) vector possesses authentic antigenicity. Vaccine. 21(27-30):4201-4209.
RAPID DETECTION OF CLASSICAL SWINE FEVER VIRUS BY A PORTABLE REAL-TIME REVERSE TRANSCRIPTASE PCR ASSAY - (Peer Reviewed Journal)
Rapid detection of classical swine fever virus by a portable real time reverse transcriptase-PCR asssay - (Peer Reviewed Journal)
Risatti, G.R., Callahan, J.D., Nelson, W.M., Borca, M.V. 2003. Rapid detection of classical swine fever virus by a portable real time reverse transcriptase-PCR asssay. Journal of Clinical Microbiology. 41(1):500-505. doi: 10.1128/jcm.41.1.500-505.2003.
ARS News Articles