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ARS Home » Northeast Area » Ithaca, New York » Robert W. Holley Center for Agriculture & Health » Emerging Pests and Pathogens Research » People & Locations » Michelle Heck
Michelle Heck
Emerging Pests and Pathogens Research
Research Molecular Biologist

Phone: (607) 254-5453
Fax: (607) 255-1132

(Employee information on this page comes from the REE Directory. Please contact your front office staff to update the REE Directory.)

Projects
Therapeutic Molecule Evaluation and Field Delivery Pipeline for Solutions to HLB
Cooperative Agreement (A)
  Accession Number: 444671
SP: Molecular Strategies to Block Psyllid Transmission of the Huanglongbing (HLB) Pathogen
Cooperative Agreement (A)
  Accession Number: 445097
Grove Evaluation of Symbiont Technology
Cooperative Agreement (A)
  Accession Number: 445321
Evaluation of Symbiont Technology in Florida Citrus Groves
Cooperative Agreement (A)
  Accession Number: 445323
CAP: Therapeutic Molecule Evaluation and Field Delivery Pipeline for Solutions to HLB
Cooperative Agreement (A)
  Accession Number: 438915
Leveraging a Structure-Function Approach to Block Polerovirus Transmission by Aphids
Cooperative Agreement (A)
  Accession Number: 439060
Development of Biological Control Tools to Manage Insect Vector Borne Plant Pathogens
Cooperative Agreement (A)
  Accession Number: 447133
Greenhouse and Field Evaluation of Therapeutic Solutions to Citrus HLB
Cooperative Agreement (A)
  Accession Number: 437746
Interfering with Insect Vector Transmission of Plant Pathogens Using RNA-based Technology
Cooperative Agreement (A)
  Accession Number: 441074
Cap: Therapeutic Molecule Evaluation And Field Delivery Pipeline For Solutions To HLB
Cooperative Agreement (A)
  Accession Number: 441865
Development of Molecular Tools to Control Citrus Greening Disease
Cooperative Agreement (A)
  Accession Number: 443161
Cap: Therapeutic Molecule Evaluation and Field Delivery Pipeline for Solutions to HLB
Cooperative Agreement (A)
  Accession Number: 439557
SP: Molecular Strategies to Block Psyllid Transmission of the Huanglongbing (HLB) Pathogen
Cooperative Agreement (A)
  Accession Number: 443725
Management and Biology of Arthropod Pests and Arthropod-borne Plant Pathogens
In-House Appropriated (D)
  Accession Number: 439292
Proteomic, Genomic and Biological Characterization of Emerging Viruses in Cotton
Interagency Reimbursable Agreement (I)
  Accession Number: 446222
Leveraging a Structure-Function Approach to Block Polerovirus Transmission by Aphids
Interagency Reimbursable Agreement (I)
  Accession Number: 438588
SP: Molecular Strategies to Block Psyllid Transmission of the Huanglongbing (HLB) Pathogen
Interagency Reimbursable Agreement (I)
  Accession Number: 443440
Therapeutic Molecule Evaluation and Field Delivery Pipeline for Solutions to HLB
Interagency Reimbursable Agreement (I)
  Accession Number: 439266
Therapeutic Molecule Evaluation and Field Delivery Pipeline for Solutions to HLB
Interagency Reimbursable Agreement (I)
  Accession Number: 439270
Characterization of Virus Populations Associated with Cotton Leafroll Dwarf Virus-Infected Plants in Cotton
Interagency Reimbursable Agreement (I)
  Accession Number: 444150
New Approaches to Control Development of Novel Management Tools for Cotton Leafroll Dwarf Virus and its Insect Vectors Using Molecular Virology
Interagency Reimbursable Agreement (I)
  Accession Number: 439720
A Holistic Time-Series Analysis of Stony Coral Tissue Loss Disease
Reimbursable Cooperative Agreement (R)
  Accession Number: 445480

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Data mining redefines the timeline and geographic spread of cotton leafroll dwarf virus - (Peer Reviewed Journal)
Understanding and Engineering Symbiont Physiology with Arabidopsis Cell Identity Lines and Post Transcriptional Gene Silencing - (Abstract Only)
Nanometal reagent therapeutics suppress Candidatus Liberibacter asiaticus, the causal pathogen of Huanglongbing - (Abstract Only)
Trunk injection of commercial oxytetracycline for HLB management in young Grapefruit trees using a novel injection system - (Abstract Only)
Flipping the HLB therapeutic screening paradigm: That’s right we went straight to the field - (Abstract Only)
Genome-wide association study reveals genes regulating vector competency of Diaphorina citri, insect vector of Huanglongbing (HLB) - (Abstract Only)
Symbiont Technology: development and optimization of a novel delivery approach for therapeutics to control Candidatus Liberibacter asiaticus - (Abstract Only)
Finding new HLB therapies: A grove-first approach - (Trade Journal)
Niedz, R.P., Locatelli, G., Larson, N.R., Rossi, L., Cochran, E., Heck, M.L. Finding new HLB therapies: A grove-first approach. Citrus Industry. 2024. 105/8-11.
Characterization of cotton virus A, a distinct member of the caulimovirus genus with endogenous viral elements in upland cotton Reprint Icon - (Pre-print Publication)
West-Ortiz, M., Stuehler, D., Pollock, E., Wilson, J.R., Preising, S., Alabi, O., Fuchs, M., Heck, M.L., Olmedo-Velarde, A. 2023. Characterization of cotton virus A, a distinct member of the caulimovirus genus with endogenous viral elements in upland cotton. bioRxiv. https://doi.org/10.1101/2023.06.14.544975.
Profiling plant proteome and transcriptome changes during grapevine fanlead virus infection Reprint Icon - (Peer Reviewed Journal)
Roy, B., Deblasio, S.L., Yang, Y., Thannhauser, T.W., Heck, M.L., Fuchs, M. 2023. Profiling plant proteome and transcriptome changes during grapevine fanlead virus infection. Journal of Proteome Research. 22(6):1997-2017. https://doi.org/10.1021/acs.jproteome.3c00069?urlappend=%3Fref%3DPDF&jav=VoR&rel=cite-as.
Potato leafroll virus molecular interactions with plants and aphids: gaining a new tactical advantage on an old foe Reprint Icon - (Review Article)
Olmedo-Velarde, A., Wilson, J.R., Stallone, M., Deblasio, S.L., Chappie, J.S., Heck, M.L. 2023. Potato leafroll virus molecular interactions with plants and aphids: gaining a new tactical advantage on an old foe. Physiological and Molecular Plant Pathology. https://doi.org/10.1016/j.pmpp.2023.102015.
Grapevine fanleaf virus RNA1-encoded proteins 1A and 1BHel individually or cooperatively suppress RNA silencing Reprint Icon - (Peer Reviewed Journal)
Choi, J., Pakbaz, S., Yepes, L., Cieniewicz, E., Schmitt-Keichinger, C., Labarile, R., Minutillo, S., Heck, M.L., Hua, J., Fuchs, M. 2023. Grapevine fanleaf virus RNA1-encoded proteins 1A and 1BHel individually or cooperatively suppress RNA silencing. Molecular Plant-Microbe Interactions. https://doi.org/10.1094/MPMI-01-23-0008-R.
Potato leafroll virus modulates aphid infection with a newly described insect flavivirus Reprint Icon - (Peer Reviewed Journal)
Larrea-Sarmiento, A., Olmedo-Velarde, A., Preising, S., West-Ortiz, M., Fei, Z., Heck, M.L. 2023. Potato leafroll virus modulates aphid infection with a newly described insect flavivirus. Phytopathology. 125:102015. https://doi.org/10.1016/j.pmpp.2023.102015.
A perspective on current therapeutic molecule screening methods against ‘candidatus liberibacter asiaticus’, the presumed causative agent of citrus Huanglongbing Reprint Icon - (Peer Reviewed Journal)
Kennedy, J.P., Wood, K., Pitino, M., Mandadi, K., Igwe, D., Shatters, R.G., Widmer, T.L., Niedz, R.P., Heck, M.L. 2023. A perspective on current therapeutic molecule screening methods against ‘candidatus liberibacter asiaticus’, the presumed causative agent of citrus Huanglongbing. Phytopathology. https://doi.org/10.1094/PHYTO-12-22-0455-PER.
Polerovirus N-terminal readthrough domain structures reveal novel molecular strategies for mitigating virus transmission by aphids Reprint Icon - (Peer Reviewed Journal)
Schiltz, C.J., Wilson, J.R., Hosford, C., Adams, M.C., Preising, S.E., Deblasio, S.L., Maccleod, H.J., Ven Eck, J., Heck, M.L., Chappie, J.S. 2022. Polerovirus N-terminal readthrough domain structures reveal novel molecular strategies for mitigating virus transmission by aphids. Nature Communications. 13:6368. https://doi.org/10.1038/s41467-022-33979-2.
Strain tracking of ‘candidatus liberibacter asiaticus’, citrus greening disease pathogen, enabled by high-resolution microbiome analysis of the Asian citrus psyllid Reprint Icon - (Peer Reviewed Journal)
Higgins, S., Mann, M., Heck, M.L. 2022. Strain tracking of ‘candidatus liberibacter asiaticus’, citrus greening disease pathogen, enabled by high-resolution microbiome analysis of the Asian citrus psyllid. Phytopathology. 112(11):2273-2287. https://doi.org/10.1094/PHYTO-02-22-0067-R.
Transmission of grapevine red blotch virus by spissistilus festinus [Say, 1830] (Hemiptera: Membracidae) between free-living vines and vitis vinifera ‘cabernet franc’ Reprint Icon - (Peer Reviewed Journal)
Hoyle, V., Flasco, M., Choi, J., Cieniewicz, E., Mclane, H., Perry, K., Dangl, G., Rwahnih, M., Heck, M.L., Loeb, G., Fuchs, M. 2022. Transmission of grapevine red blotch virus by spissistilus festinus [Say, 1830] (Hemiptera: Membracidae) between free-living vines and vitis vinifera ‘cabernet franc’. Viruses. 14: Article e1156. https://doi.org/10.3390/v14061156.
Polerovirus N-terminal readthrough domain structures reveal novel molecular strategies for mitigating virus transmission by aphids Reprint Icon - (Pre-print Publication)
Schiltz, C.J., Wilson, J.R., Hosford, C.J., Adams, M., Preising, S., Deblasio, S.L., Maccleod, H.J., Van Eck, J., Heck, M.L., Chappie, J.S. 2022. Polerovirus N-terminal readthrough domain structures reveal novel molecular strategies for mitigating virus transmission by aphids. bioRxiv. https://doi.org/10.1101/2022.04.10.487772.
Lessons learned about the biology and genomics of Diaphorina citri infection with “Candidatus Liberibacter asiaticus” by integrating new and archived organ-specific transcriptome data Reprint Icon - (Peer Reviewed Journal)
Mann, M., Saha, S., Pitino, M., Moulton, K.M., Cano, L., Hunter, W.B., Mueller, L.A., Heck, M.L. 2022. Lessons learned about the biology and genomics of Diaphorina citri infection with “Candidatus Liberibacter asiaticus” by integrating new and archived organ-specific transcriptome data. Gigascience. 11:1-16. https://doi.org/10.1093/gigascience/giac035.
Quantitative isotope-labeled cross-linker proteomics reveals developmental variation in protein interactions and post-translational modifications in diaphorina citri, the citrus greening insect vector Reprint Icon - (Peer Reviewed Journal)
Ramsey, J.S., Zhong, X., Saha, S., Chavez, J., Johnson, R., Mahoney, J., Keller, A., Moulton, K., Mueller, L., Hall, D.G., Maccoss, M., Bruce, J., Heck, M.L. 2022. Quantitative isotope-labeled cross-linker proteomics reveals developmental variation in protein interactions and post-translational modifications in diaphorina citri, the citrus greening insect vector. ACS Agricultural Science and Technology. 2(3):486-500. https://doi.org/10.1021/acsagscitech.1c00264.
Crystal structure of the potato leafroll virus coat protein and implications for viral assembly Reprint Icon - (Peer Reviewed Journal)
Adams, M., Schiltz, C., Heck, M.L., Chappie, J. 2021. Crystal structure of the potato leafroll virus coat protein and implications for viral assembly. Journal of Structural Biology. 214(1):107811. https://doi.org/10.1016/j.jsb.2021.107811.
Host plant adaptation drives changes in Diaphorina citri proteome regulation, proteoform expression and transmission of Candidatus Liberibacter asiaticus, the citrus greening pathogen Reprint Icon - (Peer Reviewed Journal)
Ramsey, J.S., Ammar, D., Mahoney, J.E., Rivera, K., Johnson, R., Igwe, D.O., Thannhauser, T.W., Maccoss, M.J., Hall, D.G., Heck, M.L. 2022. Host plant adaptation drives changes in Diaphorina citri proteome regulation, proteoform expression and transmission of Candidatus Liberibacter asiaticus, the citrus greening pathogen. Phytopathology. 112:101-115. https://doi.org/10.1094/phyto-06-21-0275-r.
An excised leaf assay to measure acquisition of “candidatus liberibacter asiaticus” by psyllids associated with citrus Huanglongbing disease Reprint Icon - (Peer Reviewed Journal)
Igwe, D.O., Higgins, S.A., Heck, M.L. 2021. An excised leaf assay to measure acquisition of “candidatus liberibacter asiaticus” by psyllids associated with citrus Huanglongbing disease. Phytopathology. in press. https://doi.org/10.1094/PHYTO-03-21-0124-SC.
Affinity purification–mass spectrometry identifies a novel interaction between a polerovirus and a conserved innate immunity aphid protein that regulates transmission efficiency Reprint Icon - (Peer Reviewed Journal)
Deblasio, S.L., Wilson, J., Tamborindeguy, C., Johnson, R., Pinheiro, P., Maccoss, M., Gray, S.M., Heck, M.L. 2021. Affinity purification–mass spectrometry identifies a novel interaction between a polerovirus and a conserved innate immunity aphid protein that regulates transmission efficiency. Journal of Proteome Research. 20(6):3365-3387. https://doi.org/10.1021/acs.jproteome.1c00313.
Proteomic polyphenism in color morphotypes of diaphorina citri, insect vector of citrus greening disease Reprint Icon - (Peer Reviewed Journal)
Hosseinzadeh, S., Higgins, S.A., Ramsey, J.S., Howe, K.J., Griggs, M., Castrillo, L.A., Heck, M.L. 2021. Proteomic polyphenism in color morphotypes of diaphorina citri, insect vector of citrus greening disease. Journal of Proteome Research. 20(5):2851-2866. https://doi.org/10.1021/acs.jproteome.1c00089.
Grapevine red blotch virus is transmitted by the three-cornered alfalfa hopper in a circulative, nonpropagative mode with unique attributes Reprint Icon - (Peer Reviewed Journal)
Flasco, M., Hoyle, V., Cieniewicz, E., Roy, B., Mclane, H., Perry, K., Loeb, G., Nault, B., Heck, M.L., Fuchs, M. 2021. Grapevine red blotch virus is transmitted by the three-cornered alfalfa hopper in a circulative, nonpropagative mode with unique attributes. Phytopathology. https://doi.org/10.1094/PHYTO-02-21-0061-R.
A proteomic approach reveals possible molecular mechanisms and roles for endosymbiotic bacteria in begomovirus transmission by whiteflies Reprint Icon - (Peer Reviewed Journal)
Kliot, A., Johnson, R., Maccoss, M., Kontsedalov, S., Lebedev, G., Czosnek, H., Heck, M.L., Ghanim, M. 2020. A proteomic approach reveals possible molecular mechanisms and roles for endosymbiotic bacteria in begomovirus transmission by whiteflies. Gigascience. 9:1-10. https://doi.org/10.1093/gigascience/giaa124.
Natural products: potential for HLB control - (Peer Reviewed Journal)
Development on Citrus medica infected with ‘Candidatus Liberibacter asiaticus’ has sex-specific and -nonspecific impacts on adult Diaphorina citri and its endosymbionts Reprint Icon - (Peer Reviewed Journal)
Coates, L.C., Mahoney, J., Ramsey, J.S., Warick, E., Johnson, R., Maccoss, M., Krasnoff, S., Howe, K.J., Moulton, K.M., Saha, S., Mueller, L., Hall, D.G., Shatters, R.G., Heck, M.L., Slupsky, C. 2020. Development on Citrus medica infected with ‘Candidatus Liberibacter asiaticus’ has sex-specific and -nonspecific impacts on adult Diaphorina citri and its endosymbionts. PLOS ONE. 15(1):e0239771. https://doi.org/10.1371/journal.pone.0239771.
Bacterial vector-borne plant diseases: unanswered questions and future directions Reprint Icon - (Peer Reviewed Journal)
Huang, W., Reyes-Caldas, P., Mann, M., Seifbarghi, S., Kahn, A., Almeida, R., Beven, L., Heck, M.L., Hogenhout, S., Coaker, G. 2020. Bacterial vector-borne plant diseases: unanswered questions and future directions. Molecular Plant. https://doi.org/10.1016/j.molp.2020.08.010.
Proteomics in non-model organisms: A new analytical frontier Reprint Icon - (Peer Reviewed Journal)
Heck, M.L., Neely, B. 2020. Proteomics in non-model organisms: A new analytical frontier. Journal of Proteome Research. 19:3595-3606. https://doi.org/10.1021/acs.jproteome.0c00448?ref=pdf.
Longitudinal transcriptomic, proteomic, and metabolomic analysis of Citrus limon response to graft inoculation by ‘Candidatus Liberibacter asiaticus’ Reprint Icon - (Peer Reviewed Journal)
Ramsey, J.S., Chin, E., Chavez, J., Saha, S., Mischuk, D., Mahoney, J., Mohr, J., Robison, F., Godfrey, K., Levesque, C., Foster, L., Xu, Y., Strickler, S., Fernandez, N., Polek, M., Giovannoni, J.J., Mueller, L., Slupsky, C., Bruce, J., Heck, M.L. 2020. Longitudinal transcriptomic, proteomic, and metabolomic analysis of Citrus limon response to graft inoculation by ‘Candidatus Liberibacter asiaticus’. Journal of Proteome Research. 19:2247-2263. https://doi.org/10.1021/acs.jproteome.9b00802.
Asian citrus psyllid: biology, ecology and management of the huanglongbing vector Reprint Icon - (Book / Chapter)
Ammar, E., Heck, M.L., Shatters, R.G. 2020. Asian citrus psyllid: biology, ecology and management of the huanglongbing vector. Book Chapter. 127-155. https://doi.org/10.1079/9781786394088.0113.
Peptidomics approaches for the identification of bioactive molecules from Diaphorina citri Reprint Icon - (Peer Reviewed Journal)
Fleites, L.A., Johnson, R., Kruse, A.R., Nachman, R.J., Hall, D.G., Maccoss, M., Heck, M.L. 2020. Peptidomics approaches for the identification of bioactive molecules from Diaphorina citri . Journal of Proteome Research. 19/4; 1392-1408. https://doi.org/10.1021/acs.jproteome.9b00509.
Peptidomics-driven insights into Diaphorina citri physiology and response to ‘Candidatus Liberibacter asiaticus’ Reprint Icon - (Peer Reviewed Journal)
Fleites, L.A., Igwe, D., Johnson, R., Hall, D.G., Nachman, R.J., Maccoss, M., Heck, M.L. 2020. Peptidomics-driven insights into Diaphorina citri physiology and response to ‘Candidatus Liberibacter asiaticus’. Journal of Proteome Research. https://doi.org/10.1021/acs.jproteome.9b00509.
Siderophores from the Entomopathogenic Fungus Beauveria bassiana Reprint Icon - (Peer Reviewed Journal)
Krasnoff, S., Howe, K.J., Heck, M.L., Donzelli, B. 2020. Siderophores from the Entomopathogenic Fungus Beauveria bassiana. Journal of Natural Products. 83(2):296-304.
Longitudinal transcriptomic, proteomic, and metabolomic analyses of Citrus sinensis (L.) Osbeck graft-inoculated with ‘Candidatus Liberibacter asiaticus’ Reprint Icon - (Peer Reviewed Journal)
Chin, E., Ramsey, J.S., Mishchuk, D.O., Saha, S., Mitrovic, E., Chavez, J.D., Howe, K.J., Zhong, X., Polek, M., Godfrey, K.E., Mueller, L.A., Bruce, J.E., Heck, M.L., Slupsky, C.M. 2019. Longitudinal transcriptomic, proteomic, and metabolomic analyses of Citrus sinensis (L.) Osbeck graft-inoculated with ‘Candidatus Liberibacter asiaticus’. Journal of Proteome Research. 19(2):719-732. https://doi.org/10.1021/acs.jproteome.9b00616.
Assessing protein sequence database suitability using de novosequencing Reprint Icon - (Peer Reviewed Journal)
Johnson, R., Searle, B.C., Nunn, B.L., Gilmore, J.M., Phillips, M., Amemiya, C.T., Heck, M.L., Maccoss, M.J. 2020. Assessing protein sequence database suitability using de novosequencing. Molecular and Cellular Proteomics. http://dx.doi.org/10.1074/mcp.TIR119.001752.
Lessons from one fastidious bacterium to another: What can we learn about Liberibacter species from Xylella fastidiosa Reprint Icon - (Review Article)
Kruse, A., Fleites, L.A., Heck, M.L. 2019. Lessons from one fastidious bacterium to another: What can we learn about Liberibacter species from Xylella fastidiosa. Insects. 10(9):300. https://doi.org/10.3390/insects10090300.
Color morphology of Diaphorina citri influences interactions with its bacterial endosymbionts and 'Candidatus Liberibacter asiaticus' Reprint Icon - (Peer Reviewed Journal)
Hosseinzadeh, S., Ramsey, J.S., Mann, M., Bennett, L., Hunter, W.B., Shams-Bakhsh, M., Hall, D.G., Heck, M.L. 2019. Hemocyanin protein expression is correlated with color morphology and immunity to ‘Candidatus Liberibacter asiaticus’ in the Huanglongbing insect vector, Diaphorina citri. PLoS One. 14(5):e0216599. https://doi.org/10.1371/journal.pone.0216599.
The identify of a Single Residue of the RNA-dependent RNA Polymerase of Grapevine Fanleaf Virus modulates vein clearing symptoms in Nicotiana benthamiana Reprint Icon - (Peer Reviewed Journal)
Osterbaan, L., Choi, J., Kenney, J., Flasco, M., Vigne, E., Schmitt-Keichinger, C., Rebelo, A., Heck, M.L., Fuchs, M. 2019. The identify of a Single Residue of the RNA-dependent RNA Polymerase of Grapevine Fanleaf Virus modulates vein clearing symptoms in Nicotiana benthamiana. Molecular Plant-Microbe Interactions. 32:7. https://doi.org/10.1094/MPMI-12-18-0337-R.
The Hunt for Early HLB Biomarkers - (Trade Journal)
Heck, M.L. 2019. The Hunt for Early HLB Biomarkers. Citrograph. 10(2):26-29.
Plant viruses transmitted in two different modes produce differing effects on small rna-mediated processes in their aphid vector Reprint Icon - (Peer Reviewed Journal)
Pinheiro, P.V., Wilson, J.R., Xu, Y., Zheng, Y., Rebelo, A., Fattah-Houseini, S., Kruse, A., Santos Dos Silva, R., Xu, Y., Kramer, M.H., Giovannoni, J.J., Fei, Z., Gray, S.M., Heck, M.L. 2019. Plant viruses transmitted in two different modes produce differing effects on small rna-mediated processes in their aphid vector. Phytobiomes Journal. 3(1):71-81. https://doi.org/10.1094/PBIOMES-10-18-0045-R.
Looking through the lens of ‘omics technologies: Insights into the transmission of insect vector-borne plant viruses Reprint Icon - (Book / Chapter)
Wilson, J.R., Deblasio, S.L., Alexander, M.M., Heck, M.L. 2019. Looking through the lens of ‘omics technologies: Insights into the transmission of insect vector-borne plant viruses. Insect Molecular Biology. 6(34):113-144. https://doi.org/10.21775/cimb.034.113.
Looking through the lens of ‘omics technologies: Insights into the transmission of insect vector-borne plant viruses Reprint Icon - (Peer Reviewed Journal)
Wilson, J.R., Deblasio, S.L., Alexandr, M.M., Heck, M.L. 2019. Looking through the lens of ‘omics technologies: Insights into the transmission of insect vector-borne plant viruses. Current Issues in Molecular Biology. 34:113-144. https://doi.org/10.21775/cimb.034.113.
Distribution and variation of bacterial endosymbiont and “candidatus liberibacter asiaticus” titer in the huanglongbing insect vector, diaphorina citri kuwayama Reprint Icon - (Peer Reviewed Journal)
Hosseinzadeh, S., Shams-Bakhsh, M., Mann, M., Fattah-Hosseini, S., Bagheri, A., Heck, M.L. 2018. Distribution and variation of bacterial endosymbiont and “candidatus liberibacter asiaticus” titer in the huanglongbing insect vector, diaphorina citri kuwayama. Microbial Ecology. 78(1):206-222. https://doi.org/10.1007/s00248-018-1290-1.
The interaction dynamics of two potato leafroll virus movement proteins affects their localization to the outer membranes of mitochondria and plastids Reprint Icon - (Peer Reviewed Journal)
DeBlasio, S.L., Xu, Y., Johnson, R., Rebelo, A., MacCoss, M., Gray, S.M., Heck, M.L. 2018. The interaction dynamics of two potato leafroll virus movement proteins affects their localization to the outer membranes of mitochondria and plastids. Viruses. 10(11):585. https://doi.org/10.3390/v10110585.
‘Candidatus Liberibacter asiaticus’ minimally alters expression of immunity and metabolism proteins in the hemolymph of Diaphorina citri, the insect vector of Huanglongbing Reprint Icon - (Peer Reviewed Journal)
Kruse, A., Ramsey, J.S., Johnson, R., Hall, D.G., MacCoss, M., Heck, M.L. 2018. ‘Candidatus Liberibacter asiaticus’ minimally alters expression of immunity and metabolism proteins in the hemolymph of Diaphorina citri, the insect vector of Huanglongbing. Journal of Proteome Research. 17(9):2995-3011. https://doi.org/10.1021/acs.jproteome.8b00183.
Bacteria associated with Russian Wheat Aphid (Diuraphis noxia) enhance aphid virulence to wheat Reprint Icon - (Peer Reviewed Journal)
Luna, E., Van Eck, L., Campillo, T., Weinroth, M., Metcalf, J., Perez-Quintero, A.L., Botha, A., Thannhauser, T.W., Pappin, D., Tissarat, N.A., Lapitan, N.L., Argueso, C.T., Ode, P.J., Heck, M.L., Leach, J.E. 2018. Bacteria associated with Russian Wheat Aphid (Diuraphis noxia) enhance aphid virulence to wheat. Phytobiomes Journal. https://doi.org/10.1094/PBIOMES-06-18-0027-R.
Targeted disruption of aphid transmission: A vision for the management of crop diseases caused by Luteoviridae members Reprint Icon - (Peer Reviewed Journal)
Heck, M.L., Brault, V. 2018. Targeted disruption of aphid transmission: A vision for the management of crop diseases caused by Luteoviridae members. Current Opinion in Virology. 33:24-32. https://doi.org/10.1016/j.coviro.2018.07.007.
Disruption of chloroplast function through downregulation of phytoene desaturase enhances the systemic accumulation of an aphid-borne, phloem-restricted virus Reprint Icon - (Peer Reviewed Journal)
DeBlasio, S.L., Rebelo, A., Parks, K., Gray, S.M., Heck, M.L. 2018. Disruption of chloroplast function through downregulation of phytoene desaturase enhances the systemic accumulation of an aphid-borne, phloem-restricted virus. Molecular Plant-Microbe Interactions. https://doi.org/10.1094/MPMI-03-18-0057-R.
The quest for a non-vector psyllid: Natural variation in acquisition and transmission of the huanglongbing pathogen 'Candidatus Liberibacter asiaticus' by Asian citrus psyllid isofemale lines Reprint Icon - (Peer Reviewed Journal)
Ammar, E., Hall, D.G., Hosseinzadeh, S., Heck, M.L. 2018. The quest for a non-vector psyllid: Natural variation in acquisition and transmission of the huanglongbing pathogen 'Candidatus Liberibacter asiaticus' by Asian citrus psyllid isofemale lines. PLoS One. 13(4):e0195804. https://doi.org/10.1371/journal.pone.0195804.
A stem-loop structure in potato leafroll virus ORF5 that is essential for readthrough translation of the coat protein ORF stop codon 700 bases upstream Reprint Icon - (Peer Reviewed Journal)
Xu, Y., Ju, H., DeBlasio, S.L., Carino, E.J., Johnson, R., MacCoss, M., Heck, M.L., Miller, W., Gray, S.M. 2018. A stem-loop structure in potato leafroll virus ORF5 that is essential for readthrough translation of the coat protein ORF stop codon 700 bases upstream. Journal of Virology. https://doi.org/10.1128/JVI.01544-17.
Crystal structure of diaphorin methanol monosolvaate isolated from diaphorina citri kuwayama, the insect vector of citrus greening disease Reprint Icon - (Peer Reviewed Journal)
Szebenyi, D., Kriksunov, I., Howe, K.J., Ramsey, J.S., Hall, D.G., Heck, M.L., Krasnoff, S. 2018. Crystal structure of diaphorin methanol monosolvaate isolated from diaphorina citri kuwayama, the insect vector of citrus greening disease. Acta Crystallographica Secion E. 74(Pt 4):445-449. https://doi.org/10.1107/S2056989018002992.
Diaphorina citri nymphs are resistant to morphological changes induced by “Candidatus Liberibacter asiaticus” in midgut epithelial cells Reprint Icon - (Peer Reviewed Journal)
Mann, M., Fattah-Hosseini, S., Ammar, E., Strange, R., Warrick, E., Sturgeon, K., Shatters, R.G., Heck, M.L. 2018. Diaphorina citri nymphs are resistant to morphological changes induced by “Candidatus Liberibacter asiaticus” in midgut epithelial cells. Infection and Immunity. 86(4):e00889-17. https://doi.org/10.1128/IAI.00889-17.
Insect transmission of plant pathogens: a systems biology perspective Reprint Icon - (Peer Reviewed Journal)
Heck, M.L. 2018. Insect transmission of plant pathogens: a systems biology perspective. mSystems. 3(2). https://doi.org/10.1128/mSystems.00168-17.
Protein interaction networks at the host-microbe interface in Diaphorina citri, the insect vector of the citrus greening pathogen Reprint Icon - (Proceedings)
Ramsey, J.S., Chavez, J., Johnson, R., Mahoney, J., Mohr, J., Robison, F., Zhong, X., Hall, D.G., Maccoss, M., Bruce, J., Heck, M.L. 2017. Protein interaction networks at the host-microbe interface in Diaphorina citri, the insect vector of the citrus greening pathogen. International Research Conference on Huanglongbing. 4(2):160545. https://doi.org/10.1098/rsos.160545.
Relative susceptibility of Musa genotypes to banana bunchy top disease in Cameroon and implication for disease management - (Peer Reviewed Journal)
Ngatat, S., Hanna, R., Kumar, P.L., Gray, S.M., Cilia, M., Ghogomu, R.T., Fontem, D.A. 2017. Relative susceptibility of Musa genotypes to banana bunchy top disease in Cameroon and implication for disease management. Crop Protection. 101:116-122.
Evaluation of a bead-free immunoprecipitation technique coupled with tandem mass spectrometry for identification of plant-virus protein interactions Reprint Icon - (Peer Reviewed Journal)
DeBlasio, S.L., Bereman, M.S., Mahoney, J., Thannhauser, T.W., Gray, S.M., MacCoss, M.J., Heck, M.L. 2017. Evaluation of a bead-free immunoprecipitation technique coupled with tandem mass spectrometry for identification of plant-virus protein interactions. Journal of Biomolecular Techniques. 28(3):111-121. https://doi.org/10.7171/jbt.17-2803-002.
Combining 'omics and microscopy to visualize interactions between the Asian citrus psyllid vector and the Huanglongbing pathogen Candidatus Liberibacter asiaticus in the insect gut - (Peer Reviewed Journal)
Kruse, A., Fattah-Hosseini, S., Saha, S., Johnson, R., Warwick, E., Sturgeon, K., Mueller, L., Maccoss, M.J., Shatters, R.G., Cilia, M. 2017. Combining 'omics and microscopy to visualize interactions between the Asian citrus psyllid vector and the Huanglongbing pathogen Candidatus Liberibacter asiaticus in the insect gut. PLoS One. https://doi.org/10.1371/journal.pone.0179531.
A molecular tug-of-war: Global plant proteome changes during viral infection - (Peer Reviewed Journal)
Alexander, M., Cilia, M. 2017. A molecular tug-of-war: Global plant proteome changes during viral infection. Current Plant Biology. 5:13-24.
Insights in luteovirid structural biology guided by chemical cross-linking and high resolution mass spectrometry Reprint Icon - (Peer Reviewed Journal)
Alexander, M., Mohr, J.P., DeBlasio, S.L., Chavez, J., Ziegler-Graff, V., Brault, V., Bruce, J., Heck, M.L. 2017. Insights in luteovirid structural biology guided by chemical cross-linking and high resolution mass spectrometry. Virus Research. 241:42-52. https://doi.org/10.1016/j.virusres.2017.05.005.
Improved annotation of the insect vector of citrus greening disease: biocuration by a diverse genomics community Reprint Icon - (Peer Reviewed Journal)
Saha, S., Hosmani, P.S., Villalobos-Ayala, K., Miller, S., Shippy, T., Flores, M., Rosendale, A., Shatters, R.G., D'Elia, T.D., Brown, S.J., Hunter, W.B., Heck, M.L. 2019. Improved annotation of the insect vector of citrus greening disease: biocuration by a diverse genomics community. Database: The Journal of Biological Databases and Curation. 2019. https://doi.org/10.1093/database/baz035.
Protein interaction networks at the host-microbe interface in Diaphorina citri, the insect vector of the citrus greening pathogen - (Peer Reviewed Journal)
Ramsey, J.S., Chavez, J., Johnson, R., Mahoney, J., Mohr, J., Robison, F., Zhong, X., Hall, D.G., Maccoss, M., Bruce, J., Cilia, M. 2017. Protein interaction networks at the host-microbe interface in Diaphorina citri, the insect vector of the citrus greening pathogen. Royal Society Open Science. https://doi.org/10.1098/rsos.160545.
Host plants indirectly influence plant virus transmission by altering gut cysteine protease activity of aphid vectors - (Peer Reviewed Journal)
Pinheiro, P., Ghanim, M., Rebelo, A., Santos, R., Orsburn, B.C., Gray, S.M., Cilia, M. 2016. Host plants indirectly influence plant virus transmission by altering gut cysteine protease activity of aphid vectors. Molecular and Cellular Proteomics. DOI: 10.1074/mcp.M116.063495.
Improved annotation of the insect vector of citrus greening disease: biocuration by a diverse genomics community Reprint Icon - (Peer Reviewed Journal)
Saha, S., Hosmani, P.S., Billalobos-Ayala, K., Miller, S., Shippy, T., Flores, M., Rosendale, A., Cordola, C., Bell, T., Mann, H., DeAvila, G., DeAvila, D., Moore, Z., Buller, K., Ciolkevich, K., Nandyal, S., Mahoney, R., Van Voorhis, J., Dunlevy, M., Farrow, D., Hunter, D., Morgan, T., Shore, K., Guzman, V., Izsak, A., Dixon, D.E., Cridge, A., Cano, L., Cao, X., Jiang, H., Leng, N., Johnson, S., Cantarel, B.L., Richards, S., English, A., Shatters, R.G., Childers, C., Chen, M., Cilia, M., Mueller, L., Munoz-Torres, M., Nelson, D., Poelchau, M.F., Benoit, J., Wiersma-Koch, Brown, S.J. 2017. Improved annotation of the insect vector of citrus greening disease: biocuration by a diverse genomics community. bioRxiv. https://doi.org/10.1093/database/bax032.
A general method for targeted quantitative cross-linking mass spectrometry - (Peer Reviewed Journal)
Chavez, J.D., Eng, J.K., Cilia, M., Rivera, K., Zhong, X., Wu, X., Schweppe, D., Allen, T., Khurgel, M., Kumar, A., Lampropoulos, A., Larsson, M., Maity, S., Morozov, Y., Pathmasiri, W., Perez-Neut, M., Pineyro-Ruiz, C., Polina, E., Post, S., Rider, M., Tokmina-Roszyk, D., Parrine Sant'Ana, D., Bruce, J.E. 2016. A general method for targeted quantitative cross-linking mass spectrometry. PLoS One. 11(12):e0167547.
The draft genome of whitefly Bemisia tabaci MEAM1, a global crop pest, provides novel insights into virus transmission, host adaptation, and insecticide resistance - (Peer Reviewed Journal)
Chen, W., Hasegawa, D.K., Kaur, N., Kliot, A., Pinheiro, P.V., Luan, J., Stensmyr, M.C., Zheng, Y., Liu, W., Sun, H., Xu, Y., Luo, Y., Kruse, A., Yang, X., Kontsedalov, S., Lebedev, G., Fisher, T., Nelson, D.R., Hunter, W.B., Brown, J.K., Jander, G., Cilia, M., Douglas, A.E., Ghanim, M., Simmons, A.M., Wintermantel, W.M., Ling, K., Fei, Z. 2016. The draft genome of whitefly Bemisia tabaci MEAM1, a global crop pest, provides novel insights into virus transmission, host adaptation, and insecticide resistance. BMC Biology. 14:110 doi:10.1186/s12915-016-0321-Y.
Model system-guided protein interaction mapping for virus isolated from phloem tissue - (Peer Reviewed Journal)
Deblasio, S.L., Johnson, R., Maccoss, M., Gray, S.M., Cilia, M. 2016. Model system-guided protein interaction mapping for virus isolated from phloem tissue. Journal of General Virology. 15:4601-4611.
Morphological abnormalities and cell death in the Asian citrus psyllid (Diaphorina citri) midgut associated with Candidatus Liberibacter asiaticus Reprint Icon - (Peer Reviewed Journal)
Ghanim, M., Fattah-Hosseini, S., Levy, A., Heck, M.L. 2018. Morphological abnormalities and cell death in the Asian citrus psyllid (Diaphorina citri) midgut associated with Candidatus Liberibacter asiaticus. Scientific Reports. 6:33418. doi:10.1038/srep33418.
Factors affecting transmission rates of 'Candidatus Liberibacter asiaticus' by Asian citrus psyllid - (Abstract Only)
Visualization of host-polerovirus interaction topologies using Protein Interaction Reporter technology - (Peer Reviewed Journal)
Deblasio, S.L., Chavez, J., Alexander, M., Ramsey, J.S., Eng, J., Mahoney, J., Gray, S.M., Bruce, J., Cilia, M. 2015. Visualization of host-polerovirus interaction topologies using Protein Interaction Reporter technology. Journal of Virology. 90:1973-1987.
Metabolic interplay between the Asian citrus psyllid and its Profftella symbiont: An Achilles’ heel of the citrus greening insect vector - (Peer Reviewed Journal)
Ramsey, J.S., Johnson, R., Hoki, J., Kruse, A., Mahoney, J., Hilf, M.E., Hunter, W.B., Hall, D.G., Shroeder, F., Maccoss, M., Cilia, M. 2015. Metabolic interplay between the Asian citrus psyllid and its Profftella symbiont: An Achilles’ heel of the citrus greening insect vector. PLoS One. DOI: 10.1371/journal.pone.0140826.
Persistent, circulative transmission of begomoviruses by whitefly vectors - (Review Article)
Rosen, R., Kanakala, S., Kliot, A., Pakkianathan, B., Abu Farich, B., Santana-Magal, N., Elimelech, M., Kontsedalov, S., Lebedev, G., Cilia, M., Ghanim, M. 2015. Persistent, circulative transmission of begomoviruses by whitefly vectors. Current Opinion in Virology. 15:1-8.
The Potato leafroll virus structural proteins manipulate overlapping, yet distinct protein interaction networks during infection - (Peer Reviewed Journal)
Deblasio, S.L., Johnson, R., Sweeney, M., Karasev, A., Gray, S.M., Maccoss, M., Cilia, M. 2015. The Potato leafroll virus structural proteins manipulate overlapping, yet distinct protein interaction networks during infection. Proteomics. 15(12):2098-2112.
Is there a role for symbiotic bacteria in plant virus transmission? - (Peer Reviewed Journal)
Pinheiro, P., Kliot, A., Ghanim, M., Cilia, M. 2015. Is there a role for symbiotic bacteria in plant virus transmission? Current Opinion in Insect Science. 8:69-78.
Insights into the polerovirus-plant interactome revealed by co-immunoprecipitation and mass spectrometry - (Peer Reviewed Journal)
Deblasio, S.L., Johnson, R., Mahoney, J., Karasev, A., Gray, S.M., Maccoss, M., Cilia, M. 2014. Insights into the polerovirus-plant interactome revealed by co-immunoprecipitation and mass spectrometry. Molecular Plant-Microbe Interactions. DOI: 10.1094/MPMI-11-14-0363-R.
Evidence for lysine acetylation in the coat protein of a Polerovirus - (Peer Reviewed Journal)
Cilia, M., Johnson, R., Sweeney, M., Deblasio, S.L., Bruce, J.E., Maccoss, M.J., Gray, S.M. 2014. Evidence for lysine acetylation in the coat protein of a Polerovirus. Journal of General Virology. DOI: 10.1099/vir.0.066514-0.
An interdisciplinary approach to combat HLB - (Trade Journal)
Chin, E., Mishchuk, D.O., Bruce, J., Cilia, M., Coaker, G., Davis, C., Jin, H., Ma, W., Seller, G., Levesque, C., Godfrey, K., Slupsky, C.M. 2014. An interdisciplinary approach to combat HLB. Citrograph. Available: http://www.citrusresearch.org/citrograph-winter-2014.
Circulative, “Nonpropagative” Virus Transmission: An orchestra of virus, insect and plant derived instruments - (Peer Reviewed Journal)
Gray, S.M., Cilia, M., Ghanim, M. 2014. Circulative, “Nonpropagative” Virus Transmission: An orchestra of virus, insect and plant derived instruments. Advances in Virus Research. 89:141-99.
Evidence for the biochemical basis of host virulence in the greenbug aphid, Schizaphis graminum (Homoptera: Aphididae) - (Peer Reviewed Journal)
Pinheiro, P., Bereman, M., Burd, J., Pals, M.A., Armstrong, J.S., Howe, K.J., Thannhauser, T.W., Maccoss, M., Gray, S.M., Cilia, M. 2014. Evidence for the biochemical basis of host virulence in the greenbug aphid, Schizaphis graminum (Homoptera: Aphididae). Journal of Proteome Research. 13(4):2094–2108.
Infection of the whitefly Bemisia tabaci with Rickettsia spp. alters its interactions with Tomato yellow leaf curl virus - (Peer Reviewed Journal)
Kliot, A., Cilia, M., Czosnek, H., Ghanim, M. 2014. Infection of the whitefly Bemisia tabaci with Rickettsia spp. alters its interactions with Tomato yellow leaf curl virus. Journal of Virology. 88(10):5652-60.
Genomic and proteomic analysis of Schizaphis graminum reveals cyclophilin proteins are involved in the transmission of Cereal yellow dwarf virus - (Peer Reviewed Journal)
Tamborindeguy, C., Bereman, M., Deblasio, S.L., Igwe, D., Smith, D., White, F., Maccoss, M., Gray, S.M., Cilia, M. 2013. Genomic and proteomic analysis of Schizaphis graminum reveals cyclophilin proteins are involved in the transmission of Cereal yellow dwarf virus. PLoS One. 8(8):e71620.
The identification of protein biomarkers distinguishing virus transmission competent and refractive insect populations by coupling genetics with quantitative intact proteomics - (Proceedings)
Discovery and targeted LC-MS/MS of purified polerovirus reveals differences in the virus-host interactome associated with altered aphid transmission - (Peer Reviewed Journal)
Cilia, M., Peter, K.A., Bereman, M., Howe, K.J., Fish, T., Smith, D., Gildow, F., Maccoss, M., Gray, S.M. 2012. Discovery and targeted LC-MS/MS of purified polerovirus reveals differences in the virus-host interactome associated with altered aphid transmission. PLoS One. 7(10).
Cross-linking measurements of the potato leafroll virus reveal protein interaction topologies required for virion stability, aphid transmission, and virus-plant interactions - (Peer Reviewed Journal)
Chavez, J., Cilia, M., Ju, H., Weisbrod, C., Eng, J.K., Gray, S.M., Bruce, J.E. 2012. Cross-linking measurements of the potato leafroll virus reveal protein interaction topologies required for virion stability, aphid transmission, and virus-plant interactions. Journal of Proteome Research. 11(5):2968-2981.
Homopteran vector biomarkers for efficient circulative plant virus transmission are conserved in multiple aphid species and the whitefly Bemisia tabaci - (Peer Reviewed Journal)
Cilia, M., Bereman, M., Fish, T., Maccoss, M.J., Gray, S.M. 2012. Homopteran vector biomarkers for efficient circulative plant virus transmission are conserved in multiple aphid species and the whitefly Bemisia tabaci. Journal of Integrative Agriculture. 11(2):249-262.
Biomarker discovery from the top down: protein biomarkers for efficient virus transmission by insects (Homoptera: Aphididae) discovered by coupling genetics and 2-D DIGE - (Peer Reviewed Journal)
Cilia, M., Howe, K.J., Fish, T., Smith, D., Mahoney, J., Tamborindeguy, C., Burd, J.D., Thannhauser, T.W., Gray, S.M. 2011. Biomarker discovery from the top down: protein biomarkers for efficient virus transmission by insects (Homoptera: Aphididae) discovered by coupling genetics and 2-D DIGE. Proteomics. 11:2440-2458.
Genetics coupled to quantitative intact proteomics links heritable aphid and endosymbiont protein isoform expression to polerovirus transmission - (Peer Reviewed Journal)
Cilia, M., Tamborindeguy, C., Fish, T., Howe, K.J., Thannhauser, T.W., Gray, S.M. 2011. Genetics coupled to quantitative intact proteomics links heritable aphid and endosymbiont protein isoform expression to polerovirus transmission. Journal of Virology. 85(5):2148-2166.
Tangible benefits of the pea aphid genome sequencing in proteomics research: enhancements in protein identification, data incorporation, and evaluation criteria - (Peer Reviewed Journal)
Cilia, M., Thannhauser, T.W., Gray, S.M. 2010. Tangible benefits of the pea aphid genome sequencing in proteomics research: enhancements in protein identification, data incorporation, and evaluation criteria. Journal of Insect Physiology. Available: http://www.ncbi.nlm.nih.gov/pubmed/21070785.
The identification of protein biomarkers distinguishing virus transmission competent and refractive insect populations by coupling genetics with quantitative intact proteomics - (Proceedings)
Cilia, M., Tamborindeguy, C., Thannhauser, T.W., Gray, S.M. 2010. The identification of protein biomarkers distinguishing virus transmission competent and refractive insect populations by coupling genetics with quantitative intact proteomics. Journal of American Society for Mass Spectrometry. 58:42.
The proteomics toolbox for agricultural research: precision biomarker discovery using genetics - (Proceedings)
Cilia, M., Tamborindeguy, C., Thannhauser, T.W., Gray, S.M. 2010. The proteomics toolbox for agricultural research: precision biomarker discovery using genetics. Journal of Agricultural and Urban Entomology. 27:20.
Combined use of 2-D reverse phase chromatography & data independent mass spectrometry simultaneously characterizing proteomes of Schizaphis graminum & its obligate endosymbiont Buchnera aphidicola, from whole aphid extracts - (Abstract Only)
Mclaughlin, M., Cilia, M., Howe, K.J., Gray, S.M., Thannhauser, T.W. 2010. Combined use of 2-D reverse phase chromatography & data independent mass spectrometry simultaneously characterizing proteomes of Schizaphis graminum & its obligate endosymbiont Buchnera aphidicola, from whole aphid extracts. Journal of Biomolecular Techniques. 21(3):S58.
A Comparison of Protein Extraction Methods Suitable for Gel-Based Proteomics Studies of Aphid Proteins - (Peer Reviewed Journal)
Cilia, M., Fish, T., Yang, X., Mclaughlin, M., Thannhauser, T.W., Gray, S.M. 2009. A Comparison of Protein Extraction Methods Suitable for Gel-Based Proteomics Studies of Aphid Proteins. Journal of Biomolecular Techniques. 20(4):201-215.
Control of Arabidopsis meristem development by thioredoxin-dependent regulation of intercellular transport - (Peer Reviewed Journal)
Benitez-Alfonso, Y., Cilia, M., San Roman, A., Thomas, C., Maule, A., Hearn, S., Jackson, D. 2009. Control of Arabidopsis meristem development by thioredoxin-dependent regulation of intercellular transport. Proceedings of the National Academy of Sciences. 106(9):3615-3620.
Towards sustaining women through critical transition points in scientific careers: a workshop summary. - (Popular Publication)
Cilia, M. 2008. Towards sustaining women through critical transition points in scientific careers: a workshop summary.. Journal of Biomolecular Techniques. 19(5):353–355.
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