Robert Hnasko
Produce Safety and Microbiology Research
Research Molecular Biologist
Phone: (510) 559-5878
Fax: (510) 559-6429
(Employee information on this page comes from the REE Directory. Please contact your front office staff to update the REE Directory.)
Publications
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Complete genome Sequences of Enterococcus casseliflavus strains ASE2 and ASE4 generated by PacBio sequencing
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Tran, T.D., Lee, S., Hnasko, R.M., Mcgarvey, J.A. 2024. Complete genome Sequences of Enterococcus casseliflavus strains ASE2 and ASE4 generated by PacBio sequencing. Microbiology Resource Announcements. 0:e00660-24. https://doi.org/10.1128/mra.00660-24.
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Biocontrol of Escherichia coli O157:H7 by Enterobacter asburiae AEB30 on intact cantaloupe melons
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Tran, T.D., Lee, S., Hnasko, R.M., McGarvey, J.A. 2024. Biocontrol of Escherichia coli O157:H7 by Enterobacter asburiae AEB30 on intact cantaloupe melons. Microbial Biotechnology. 17(3). Article e14437. https://doi.org/10.1111/1751-7915.14437.
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Complete genome sequence of Citrobacter braakii ASE1 generated by PacBio sequencing
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Tran, T.D., Lee, S., Hnasko, R.M., McGarvey, J.A. 2024. Complete genome sequence of Citrobacter braakii ASE1 generated by PacBio sequencing. Microbiology Resource Announcements. 13(3). Article e01000-23. https://doi.org/10.1128/MRA.01000-23.
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Use of Pantoea agglomerans ASB05 as a biocontrol agent to inhibit the growth of Salmonella enterica on intact cantaloupe melons
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Lee, S., Tran, T.D., Hnasko, R.M., McGarvey, J.A. 2023. Use of Pantoea agglomerans ASB05 as a biocontrol agent to inhibit the growth of Salmonella enterica on intact cantaloupe melons. Journal of Applied Microbiology. 134(10). Article lxad235. https://doi.org/10.1093/jambio/lxad235. [Corrigendum: Journal of Applied Microbiology: 2023, 134(11), Article lxad254, https://doi.org/10.1093/jambio/lxad254].
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Sensitive and selective detection of peanut allergen Ara h 1 by ELISA and lateral flow immunoassay
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Hnasko, R.M., Lin, A.V., McGarvey, J.A., Mattison, C.P. 2022. Sensitive and selective detection of peanut allergen Ara h 1 by ELISA and lateral flow immunoassay. Food Chemistry. 396. Article 133657. https://doi.org/10.1016/j.foodchem.2022.133657.
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Complete genome sequence of Enterobacter asburiae strain AEB30, determined using Illumina and PacBio sequencing
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Tran, T., Hnasko, R.M., Huynh, S., Parker, C.T., Gorski, L.A., McGarvey, J.A. 2021. Complete genome sequence of Enterobacter asburiae strain AEB30, determined using Illumina and PacBio sequencing. Microbiology Resource Announcements. 10(31). Article e00562-21. https://doi.org/10.1128/MRA.00562-21.
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A rapid and sensitive lateral flow immunoassay (LFIA) for the detection of gluten in foods
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Hnasko, R.M., Jackson, E.S., Lin, A.V., Haff, R.P., McGarvey, J.A. 2021. A rapid and sensitive lateral flow immunoassay (LFIA) for the detection of gluten in foods. Journal of Food Chemistry. 355. Article 129514. https://doi.org/10.1016/j.foodchem.2021.129514.
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TOR coordinates nucleotide availability with ribosome biogenesis in plants
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Busche, M., Scarpin, R.M., Hnasko, R.M., Brunkard, J.O. 2021. TOR coordinates nucleotide availability with ribosome biogenesis in plants. The Plant Cell. 33(5):1615-1632. https://doi.org/10.1093/plcell/koab043.
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Bacillus amyloliquefaciens ALB65 inhibits the growth of Listeria monocytogenes on cantaloupe melons
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Tran, T.D., Del Cid, C., Hnasko, R.M., Gorski, L.A., McGarvey, J.A. 2020. Bacillus amyloliquefaciens ALB65 inhibits the growth of Listeria monocytogenes on cantaloupe melons. Applied and Environmental Microbiology. 87(1). Article e01926-20. https://doi.org/10.1128/AEM.01926-20.
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X-ray based sterilization of larvae of the navel orangeworm (lepidoptera:pyralaide) for sterile insect technique
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Haff, R.P., Ovchinnikova, I., Liang, P., Mahoney, N.E., Gee, W.S., Gomez, J., Toyofuku, N., Jackson, E.S., Hnasko, R.M., Light, D. 2020. X-ray based sterilization of larvae of the navel orangeworm (lepidoptera:pyralaide) for sterile insect technique. Journal of Economic Entomology. 113(4):1685-1693. https://doi.org/10.1093/jee/toaa111.
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Lateral flow immunoassay (LFIA) for the detection of lethal amatoxins from mushrooms
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Bever, C.R., Adams, C.A., Hnasko, R.M., Cheng, L.W., Stanker, L.H. 2020. Lateral flow immunoassay (LFIA) for the detection of lethal amatoxins from mushrooms. PLoS One. 15(4). https://doi.org/10.1371/journal.pone.0231781.
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Complete genomic sequences of three Salmonella enterica subsp. enterica serovar muenchen strains from an orchard in San Joaquin County, California
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Tran, T.D., Huynh, S., Parker, C., Hnasko, R.M., Gorski, L.A., Khalsa Sat, D., Brown, P., McGarvey, J.A. 2020. Complete genomic sequences of three Salmonella enterica subsp. enterica serovar muenchen strains from an orchard in San Joaquin County, California. Microbiology Resource Announcements. 9(13):e00048-20. https://doi.org/10.1128/MRA.00048-20.
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Genomic insight into natural inactivation of Shiga toxin 2 production in an environmental Escherichia coli strain producing Shiga toxin 1
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Carter, M.Q., Pham, A.C., He, X., Hnasko, R.M. 2020. Genomic insight into natural inactivation of Shiga toxin 2 production in an environmental Escherichia coli strain producing Shiga toxin 1. Foodborne Pathogens and Disease. 17(9):555-567. https://doi.org/10.1089/fpd.2019.2767.
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A rapid extraction method combined with a monoclonal antibody-based immunoassay for the detection of amatoxins
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Bever, C.R., Hnasko, R.M., Cheng, L.W., Stanker, L.H. 2019. A rapid extraction method combined with a monoclonal antibody-based immunoassay for the detection of amatoxins. Toxins. 11(12). https://doi.org/10.3390/toxins11120724.
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Rapid detection of staphylococcal enterotoxin-B by lateral flow assay
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Hnasko, R.M., McGarvey, J.A., Lin, A.V. 2019. Rapid detection of staphylococcal enterotoxin-B by lateral flow assay. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy. 38(5):209-212. https://doi.org/10.1089/mab.2019.0028.
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Use of phyllosphere associated lactic acid bacteria as biocontrol agents to reduce salmonella enterica serovar poona growth on cantaloupe melons
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McGarvey, J.A., Tran, T.D., Hnasko, R.M., Gorski, L.A. 2019. Use of phyllosphere associated lactic acid bacteria as biocontrol agents to reduce salmonella enterica serovar poona growth on cantaloupe melons. Journal of Food Protection. 82(12):2148-2153. https://doi.org/10.4315/0362-028X.JFP-19-246.
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Development and characterization of monoclonal antibodies to botulinum neurotoxin type E
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Bever, C.R., Scotcher, M., Cheng, L.W., Hnasko, R.M., Stanker, L. 2019. Development and characterization of monoclonal antibodies to botulinum neurotoxin type E. Toxins. 11:407. https://doi.org/10.3390/toxins11070407.
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Bacterial population dynamics after foliar fertilization of almond leaves
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McGarvey, J.A., Han, R., Tran, T., Hnasko, R.M., Brown, P. 2018. Bacterial population dynamics after foliar fertilization of almond leaves. Journal of Applied Microbiology. 126(3):945-953. https://doi.org/10.1111/jam.14169.
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Complete genome sequence of Lactococcus lactis subsp. lactis strain 14B4, which inhibits the growth of Salmonella enterica serotype Poona in vitro
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Tran, T.D., Huynh, S., Parker, C., Han, R., Hnasko, R.M., Gorski, L.A., McGarvey, J.A. 2018. Complete genome sequence of Lactococcus lactis subsp. lactis strain 14B4, which inhibits the growth of Salmonella enterica serotype Poona in vitro. Microbiology Resource Announcements. 7(19):e01364-18. https://doi.org/10.1128/MRA.01364-18.
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Dosage-dependent effects of Monensin on the rumen microbiota of lactating dairy cattle
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McGarvey, J.A., Place, S., Palumbo, J.D., Hnasko, R.M., Mitloehner, F. 2018. Dosage-dependent effects of Monensin on the rumen microbiota of lactating dairy cattle. Advances in Dairy Research. 8(7):e00783. https://doi.org/10.1002/mbo3.783.
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A new conjugation method used for the development of an immunoassay for amanitin, a deadly mushroom toxin
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Bever, C.R., Barnych, B., Hnasko, R.M., Cheng, L.W., Stanker, L.H. 2018. A new conjugation method used for the development of an immunoassay for amanitin, a deadly mushroom toxin. Toxins. 10(7):265. https://doi.org/10.3390/toxins10070265.
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A bioassay for the optimization of macrophage conditioned medium (MCM) as culture supplement used to promote hybridoma cell survival and growth
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Hnasko, R.M., Lin, A.V., Stanker, L.H., McGarvey, J.A. 2018. A bioassay for the optimization of macrophage conditioned medium (MCM) as culture supplement used to promote hybridoma cell survival and growth. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy. 37(3):126-133. https://doi.org/10.1089/mab.2018.0008.
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Enhanced detection of infectious prions by direct ELISA from the brains of asymptomatic animals using DRM2-118 monoclonal antibody and Gdn-HCl
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Hnasko, R.M., Lin, A.V., McGarvey, J.A., Stanker, L.H. 2018. Enhanced detection of infectious prions by direct ELISA from the brains of asymptomatic animals using DRM2-118 monoclonal antibody and Gdn-HCl. Journal of Immunological Methods. 456:38-43. https://doi.org/10.1016/j.jim.2018.02.010.
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Identification and verification of hybridoma-derived monoclonal antibody variable region sequences using recombinant DNA technology and mass spectrometry
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Babrak, L.M., McGarvey, J.A., Stanker, L.H., Hnasko, R.M. 2017. Identification and verification of hybridoma-derived monoclonal antibody variable region sequences using recombinant DNA technology and mass spectrometry. Molecular Immunology. 90:287-294. https://doi.org/10.1016/j.molimm.2017.08.014.
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Rapid microfluidic assay for the detection of botulinum neurotoxin in animal sera
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Babrak, L.M., Lin, A.V., Stanker, L.H., McGarvey, J.A., Hnasko, R.M. 2016. Rapid microfluidic assay for the detection of botulinum neurotoxin in animal sera. Toxins. 8(1):13.
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Monoclonal antibody technologies and rapid detection assays
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Hnasko, R.M., Stanker, L.H., Mcgarvey, J.A. 2015. Monoclonal antibody technologies and rapid detection assays.September 23-25, 2015 Carlsbad, CA: p. 1-44.
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Bacterial populations on the surfaces of organic and conventionally grown almond drupes
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Mcgarvey, J.A., Hnasko, R.M., Stanker, L.H., Han, R., Connel, J. 2015. Bacterial populations on the surfaces of organic and conventionally grown almond drupes. Journal of Applied Microbiology. 119:529-538.
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The western blot
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Hnasko, R.M., Hnasko, T.S. 2015. The western blot. In: Hnasko, Robert, editor. ELISA: Methods and protocols. Methods in Molecular Biology. 1st edition. New York, NY:Humana Press. p. 87-96. doi: 10.1007/978-1-4939-2742-5.
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ELISA: Methods and Protocols
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Hnasko, R.M. 2015. ELISA: Methods and Protocols. Methods in Molecular Biology. Volume 1318. New York: Humana Press. 435 p. doi: 10.1007/978-1-4939-2742-5.
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The biochemical properties of antibodies and their fragments
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Hnasko, R.M. 2015. The biochemical properties of antibodies and their fragments. In: Hnasko, R., editor. ELISA: Methods and protocols. Methods in Molecular Biology. 1st edition. New York, NY: Humana Press. p. 1-14. doi: 10.1007/978-1-4939-2742-5.
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Hybridoma technology
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Hnasko, R.M., Stanker, L.H. 2015. Hybridoma technology. In: Hnasko, R., editor. ELISA: Methods and protocols. Methods in Molecular Biology. 1st edition. New York, NY: Humana Press. p. 15-28. doi: 10.1007/978-1-4939-2742-5.
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Bioconjugation of antibodies to horseradish peroxidase (hrp)
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Hnasko, R.M. 2015. Bioconjugation of antibodies to horseradish peroxidase (hrp).In: Hnasko, R., editor. ELISA: Methods and protocols. Methods in Molecular Biology. 1st edition. New York, NY: Humana Press. p. 43-50. doi: 10.1007/978-1-4939-2742-5.
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Affinity purification of antibodies
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Hnasko, R.M., McGarvey, J.A. 2015. Affinity purification of antibodies. In: Hnasko, R., editor. ELISA Methods and Protocols. New York, NY: Humana Press. p. 29-41. doi: 10.1007/978-1-4939-2742-5.
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Rapid detection of E. coli produced shiga-like toxins by lateral flow immunoassay in multiple food matrices
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Ching, K.H., He, X., Stanker, L.H., Lin, A.V., Hnasko, R.M. 2015. Rapid detection of E. coli produced shiga-like toxins by lateral flow immunoassay in multiple food matrices. Toxins. Toxins 7:1163-1173.
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Detection of shiga toxins by lateral flow assay
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Ching, K.H., He, X., Stanker, L.H., Lin, A.V., Mcgarvey, J.A., Hnasko, R.M. 2015. Detection of shiga toxins by lateral flow assay. Toxins. 7:1163-1173. doi: 10.3390/Toxins7041163.
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Prion extraction methods: comparison of bead beating, ultrasonic disruption and repeated freeze-thaw methodologies for the recovery of functional renilla-prion fusion protein from bacteria
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Hnasko, R.M., Lin, A.V., Stanker, L.H., Bala, K., McGarvey, J.A. 2016. Prion extraction methods: comparison of bead beating, ultrasonic disruption and repeated freeze-thaw methodologies for the recovery of functional renilla-prion fusion protein from bacteria. In: Micic, M., editor. Sample Preparation Techniques for Soil, Plant, and Animal Samples. New York, NY: Humana Press. p. 389-399.
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A double-sandwich ELISA for identification of monoclonal antibodies suitable for sandwich immunoassays
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Stanker, L.H., Hnasko, R.M. 2015. A double-sandwich ELISA for identification of monoclonal antibodies suitable for sandwich immunoassays. Methods in Molecular Biology. Springer Science and Business Media, New York. 216 pp. 2015. (Book Chapter.
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The generation of monoclonal antibodies and their use in rapid diagnostic tests
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Hnasko, R.M., Stanker, L.H., Mcgarvey, J.A., Lin, A.V. 2014. The generation of monoclonal antibodies and their use in rapid diagnostic tests. Proceedings: Microfluidics Regenerated: New Approaches, Applications and Enabling Technologies. Meeting Abstract. 7(1-16).
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Bacterial population structure and dynamics during the development of almond drupes
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McGarvey, J.A., Connell, J., Stanker, L.H., Hnasko, R.M. 2014. Bacterial population structure and dynamics during the development of almond drupes. Journal of Applied Microbiology. 116:1542-1552.
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A monoclonal antibody based capture ELISA for botulinum neurotoxin serotype B: toxin detection in food
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Stanker, L.H., Scotcher, M.C., Cheng, L.W., Ching, K., Mcgarvey, J.A., Hodge, D., Hnasko, R.M. 2013. A monoclonal antibody based capture ELISA for botulinum neurotoxin serotype B: toxin detection in food. Toxins. 5(11):2212-2226.
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A polyclonal antibody based immunoassay detects seven subtypes of Shiga toxin 2 produced by escherichia coli in human and environmental samples
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He, X., Patfield, S.A., Hnasko, R.M., Rasooly, R., Mandrell, R.E. 2013. A polyclonal antibody based immunoassay detects seven subtypes of Shiga toxin 2 produced by escherichia coli in human and environmental samples. PLoS One. 8(10):e76368.
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Detection of bacterial toxins by lateral flow immunoassay
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Ching, K.H., Stanker, L.H., He, X., Hnasko, R.M. 2013. Detection of bacterial toxins by lateral flow immunoassay. p. 1-28. Irvine, CA; Biodot.
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Bacterial population dynamics during the ensiling of Medicago sativa (alfalfa) and subsequent exposure to air
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McGarvey, J.A., Franco, R., Palumbo, J.D., Hnasko, R.M., Stanker, L.H., Mitloehner, F. 2013. Bacterial population dynamics during the ensiling of Medicago sativa (alfalfa) and subsequent exposure to air. Journal of Applied Microbiology. 114:1661-1670.
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Development and characterization of six monoclonal antibodies to hemagglutinin-70 (HA70) of Clostridium botulinum and their application in a sandwich ELISA
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Scotcher, M.C., Cheng, L.W., Ching, K.H., Mcgarvey, J.A., Hnasko, R.M., Stanker, L.H. 2013. Development and characterization of six monoclonal antibodies to hemagglutinin-70 (HA70) of Clostridium botulinum and their application in a sandwich ELISA. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy. 32(1):6-15. doi: 10.1089/mab.2012.0071.
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Rapid multiplex immunoassay to distinguish botulinum neurotoxin serotypes on a single lateral flow device(Abstract)
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Novel epitopes identified by Anti-PrP monoclonal antibodies produced following immunization of Prnp0/0 Balb/cJ mice with purified scrapie prions
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Stanker, L.H., Scotcher, M.C., Lin, A.V., Mcgarvey, J.A., Prusiner, S., Hnasko, R.M. 2012. Novel epitopes identified by Anti-PrP monoclonal antibodies produced following immunization of Prnp0/0 Balb/cJ mice with purified scrapie prions. Hybridoma. 31(5):312-324. doi:10.1089/hyb.2012.0022.
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Replacement of animals in assays for detection of toxins in food(Abstract)
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Rapid and selective detection of botulinum neurotoxin serotypes-A and –B with a single immunochromatographic test strip
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Ching, K.H., Lin, A.V., Mcgarvey, J.A., Stanker, L.H., Hnasko, R.M. 2012. Rapid and selective detection of botulinum neurotoxin serotypes-A and –B with a single immunochromatographic test strip. Journal of Immunological Methods. 380:23-29.
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A rapid method to improve protein detection by indirect ELISA
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Hnasko, R.M., Lin, A.V., Mcgarvey, J.A., Stanker, L.H. 2011. A rapid method to improve protein detection by indirect ELISA. Biochemical and Biophysical Research Communications. 410(4):726-731. doi: 10.1016/j.bbrc.2011.06.005.
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Generation of Antisera to Purified Prions in Lipid Rafts
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Hnasko, R.M., Serban, A.V., Carlson, G.A., Prusiner, S.B., Stanker, L.H. 2010. Generation of Antisera to Purified Prions in Lipid Rafts. Prion. 4:294-104.
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Conformation-dependent high-affinity mAbs to prion proteins
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Stanker, L.H., Serban, A.V., Safar, J., Hnasko, R.M., Prusiner, S.B. 2010. Conformation-dependent high-affinity mAbs to prion proteins. Journal of Immunology. 185:729-737.
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Conformation-Dependent High-Affinity Monoclonal Antibodies to Prion Proteins
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Stanker, L.H., Serban, A.V., Cleveland, E., Hnasko, R.M., Azucena, L., Safar, J., Dearmond, S.J., Prusiner, S.B. 2010. Isolation and characterization of new anti-PrP monoclonal antibodies. Journal of Immunology. 185: 729-737
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Generation of Monoclonal Antibodies to Purified Prions in Lipid Rafts
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Inoculation of Scrapie with the Self-Assembling RADA-Peptide Disrupts Prion Accumulation and Extends Hamster Survival
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Hnasko, R.M., Bruederle, C.E. 2009. Inoculation of Scrapie with the Self-Assembling RADA-Peptide Disrupts Prion Accumulation and Extends Hamster Survival. PLoS ONE. 4(2): http://www.plosone.org/article/info:doi/10.1371/journal.pone.0004440
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Prion infected Meat-and-Bone Meal is still infectious after biodiesel production
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Bruederle, C.E., Hnasko, R.M., Kraemer, T., Garcia, R.A., Haas, M.J., Marmer, W.N., Carter, J.M. 2008. Prion infected Meat-and-Bone Meal is still Infectious After Biodiesel Production. PLoS One 3(8): e2969. doi:10.1371/journal.pone.0002969.
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Sensitive, Preclinical Detection of Prions in Brain by nanospray liquid chromatography/tandem mass spectrometry
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Onisko, B.C., Silva, C.J., Dynin, I.A., Erickson, M.L., Vensel, W.H., Hnasko, R.M., Requena, J.R., Carter, J.M. 2007. Sensitive, Preclinical Detection of Prions in Brain by nanospray liquid chromatography/tandem mass spectrometry. Rapid Communications in Mass Spectrometry. 21(24):4023-4026.
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Genetic disruption of dopamine production results in pituitary adenomas and severe prolactinemia
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Hnasko, T.S., Hnasko, R.M., Sotak, B.N., Kapur, R.P. 2007. Genetic disruption of dopamine production results in pituitary adenomas and severe prolactinemia. Neuroendocrinology Journal. 86(1): 48-57
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PV-1 LABELS TRANS-CELLULAR OPENINGS IN MOUSE ENDOTHELIAL CELLS AND IS NEGATIVELY REGULATED BY VEGF
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Hnasko, R.M., Carter, J.M., Medina, F., Lisanti, M. 2006. Pv-1 labels trans-cellular openings in mouse endothelial cells and is negatively regulated by vegf. Cell Cycle. 5(17):2021-28.
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PV-1 IS NEGATIVELY REGULATED BY VEGF IN THE LUNG OF CAV-1, BUT NOT CAV-2, NULL MICE
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Hnasko, R.M., Philippe, F.G., Ben-Jonathan, N., Lisanti, M.P. 2006. Pv-1 is negatively regulated by vegf in the lung of cav-1, but not cav-2, null mice. Cell Cycle 5(17):2012-20.
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Loss of Nkx2.8 deregulates progenitor cells in the large airways and leads to dysplasia
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Tian, J., Radma, M., Hnasko, R.M., Locker, J. 2006. Loss of Nkx2.8 deregulates progenitor cells in the large airways and leads to dysplasia. Cancer Research. 1:66(21):10399-407.
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ARS News Articles
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