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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Research Program Support » People & Locations » Adrienne Shircliff
Adrienne Shircliff
Research Program Support
Biological Science Technician

Phone: (515) 337-6214
Fax:

(Employee information on this page comes from the REE Directory. Please contact your front office staff to update the REE Directory.)


Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Co-detection of RNA and protein (CD3e, IBA-1, Pax5, Ki-67, or pan-cytokeratin type I/II) in formalin-fixed, paraffin-embedded tonsil tissues of pigs, cattle, and white-tailed deer Reprint Icon - (Other)
Wiarda, J.E., Shircliff, A.L., Zanella, E.L., Loving, C.L., Palmer, M.V. 2024. Co-detection of RNA and protein (CD3e, IBA-1, Pax5, Ki-67, or pan-cytokeratin type I/II) in formalin-fixed, paraffin-embedded tonsil tissues of pigs, cattle, and white-tailed deer. Protocols.io. https://doi.org/10.17504/protocols.io.n92ldr5o8g5b/v1.
CD3e protein, Pax5 protein, and Senecavirus RNA detection in formalin-fixed, paraffin-embedded (FFPE) pig tissues Reprint Icon - (Other)
Wiarda, J.E., Shircliff, A.L., Cassmann, E.D., Devries, A.C. 2024. CD3e protein, Pax5 protein, and Senecavirus RNA detection in formalin-fixed, paraffin-embedded (FFPE) pig tissues. Protocols.io. https://doi.org/10.17504/protocols.io.dm6gp9d4dvzp/v1.
IBA-1 protein, cytokeratin type I/II protein, and Senecavirus RNA detection in formalin-fixed, paraffin-embedded (FFPE) pig tissues Reprint Icon - (Other)
Wymore Brand, M.J., Shircliff, A.L., Cassmann, E.D., Devries, A.C. 2024. IBA-1 protein, cytokeratin type I/II protein, and Senecavirus RNA detection in formalin-fixed, paraffin-embedded (FFPE) pig tissues. Protocols.io. https://doi.org/10.17504/protocols.io.yxmvm9yd9l3p/v1.
Multiplexed fluorescent detection of CD3e, IBA-1, Pax5, and Ki-67 in formalin-fixed, paraffin-embedded pig lymph node tissue Reprint Icon - (Other)
Wiarda, J.E., Zanella, E.L., Shircliff, A.L. 2024. Multiplexed fluorescent detection of CD3e, IBA-1, Pax5, and Ki-67 in formalin-fixed, paraffin-embedded pig lymph node tissue. Protocols.io. https://doi.org/10.17504/protocols.io.j8nlk94yxv5r/v1.
In situ staining with antibodies cross-reactive in pigs, cattle, and white-tailed deer facilitates understanding of biological tissue status and immunopathology Reprint Icon - (Peer Reviewed Journal)
Wiarda, J.E., Zanella, E.L., Shircliff, A.L., Cassmann, E.D., Loving, C.L., Devries, A.C., Palmer, M.V. 2024. In situ staining with antibodies cross-reactive in pigs, cattle, and white-tailed deer facilitates understanding of biological tissue status and immunopathology . Veterinary Immunology and Immunopathology. https://doi.org/10.1016/j.vetimm.2024.110865.
Investigations into the growth and formation of biofilm by Leptospira biflexa at temperatures encountered during infection Reprint Icon - (Peer Reviewed Journal)
Dos Santos Ribeiro, P., Stasko, J.A., Shircliff, A.L., Fernandes, L.G., Putz, E.J., Andreasen, C., Azevedo, V., Ristow, P., Nally, J.E. 2025. Investigations into the growth and formation of biofilm by Leptospira biflexa at temperatures encountered during infection. Biofilms. https://doi.org/10.1016/j.bioflm.2024.100243.
Conserved B cell signaling, activation, and differentiation in porcine jejunal and ileal Peyer’s patches despite distinct immune landscapes Reprint Icon - (Peer Reviewed Journal)
Wiarda, J.E., Shircliff, A.L., Becker, S.R., Stasko, J.A., Sivasankaran, S.K., Ackermann, M.R., Loving, C.L. 2024. Conserved B cell signaling, activation, and differentiation in porcine jejunal and ileal Peyer’s patches despite distinct immune landscapes. Mucosal Immunology. 17 (2024) 1222-1241. https://doi.org/10.1016/j.mucimm.2024.08.005.
Butyrate receptor gene expression in myeloid cells of the lower porcine gastrointestinal tract - (Abstract Only)
Detection B cell and antibody-secreting cell populations in pigs at RNA and protein levels - (Abstract Only)
Pseudotemporal and spatial organization of B cell activation and differentiation in Peyer’s patches - (Abstract Only)
Prediction of Johne's disease state based upon quantification of T cell markers and their interaction with macrophages in the bovine intestine Reprint Icon - (Peer Reviewed Journal)
Jenvey, C.J., Shircliff, A.L., Obando Marrero, E.E., Stabel, J.R. 2021. Prediction of Johne's disease state based upon quantification of T cell markers and their interaction with macrophages in the bovine intestine. Veterinary Research. Vol 52 Article 55. https://doi.org/10.1186/s13567-021-00925-x.
Prediction of Johne's disease state based upon quantification of T cell markers and their interaction with macrophages in the bovine intestine Reprint Icon - (Peer Reviewed Journal)
Jenvey, C.J., Shircliff, A.L., Obando Marrero, E.E., Stabel, J.R. 2021. Prediction of Johne's disease state based upon quantification of T cell markers and their interaction with macrophages in the bovine intestine. Veterinary Research. https://doi.org/10.1186/s13567-021-00925-x.
Identification of a reliable fixative solution to preserve complex architecture of bacterial biofilms for scanning electron microscopy evaluation Reprint Icon - (Peer Reviewed Journal)
Dassanayake, R.P., Falkenberg, S.M., Stasko, J.A., Shircliff, A.L., Lippolis, J.D., Briggs, R.E. 2020. Identification of a reliable fixative solution to preserve complex architecture of bacterial biofilms for scanning electron microscopy evaluation. PLoS One. 15(5):e0233973. https://doi.org/10.1371/journal.pone.0233973.
Quantification of macrophages and Mycobacterium avium subsp. paratuberculosis in bovine intestinal tissue during different stages of Johne's disease. Reprint Icon - (Peer Reviewed Journal)
Jenvey, C.J., Hostetter, J.M., Shircliff, A.L., Bannantine, J.P., Stabel, J.R. 2019. Quantification of macrophages and Mycobacterium avium subsp. paratuberculosis in bovine intestinal tissue during different stages of Johne's disease. Veterinary Pathology. https://doi.org/10.1177/0300985819844823.
Relationship between the pathology of bovine intestinal tissue and current diagnostic tests for Johne's disease Reprint Icon - (Peer Reviewed Journal)
Jenvey, C.J., Hostetter, J.M., Shircliff, A.L., Bannantine, J.P., Stabel, J.R. 2018. Relationship between the pathology of bovine intestinal tissue and current diagnostic tests for Johne's disease. Veterinary Immunology and Immunopathology. 202:93-101. https://doi.org/10.1016/j.vetimm.2018.06.012.
Disease state influences the presence of macrophages and Mycobacterium avium subsp. paratuberculosis in bovine intestinal tissue - (Abstract Only)