Research Project: Host-Pathogen Interactions in Fungal Diseases of Wheat and Barley

Location: Cereal Crops Research

Publications (Clicking on the reprint icon will take you to the publication reprint.)

Registration of ND VitPro Hard Red Spring Wheat - (Peer Reviewed Journal)
Green, A.J., Mergoum, M., Frohberg, R., Underdahl, J., Walz, A., Simsek, S., Otteson, B., Heilman-Morales, A., Friskop, A., Rickertsen, J., Friesen, T.L., Rouse, M.N., Jin, Y., Chao, S. 2022. Registration of ND VitPro hard red spring wheat. Journal of Plant Registrations. http://doi.org/10.1002/plr2.20239.

Association mapping reveals a reciprocal virulence/avirulence locus within diverse US Pyrenophora teres f. maculata isolates - (Peer Reviewed Journal)
Clare, S., Duellman, K., Richards, J., Poudel, R., Merrick, L., Friesen, T.L., Brueggeman, R.S. 2022. Association mapping reveals a reciprocal virulence/avirulence locus within diverse US Pyrenophora teres f. maculata isolates. BMC Genomics. 23. Article 285. https://doi.org/10.1186/s12864-022-08529-1.

Two complete genomes of Xanthomonas translucens pv. translucens isolated from barley - (Peer Reviewed Journal)
Schachterle, J.K., Shi, G., Baldwin, T., Liu, Z. 2022. Two complete genomes of Xanthomonas translucens pv. translucens isolated from barley. Microbiology Resource Announcements. https://doi.org/10.1128/mra.00010-22.

Identification of novel virulence factors in Erwinia amylovora through temporal transcriptomic analysis of infected apple flowers under field conditions - (Peer Reviewed Journal)
Schachterle, J.K., Gdanetz, K., Pandya, I., Sundin, G.W. 2022. Identification of novel virulence factors in Erwinia amylovora through temporal transcriptomic analysis of infected apple flowers under field conditions. Molecular Plant Pathology. 1-15. https://doi.org/10.1111/mpp.13199.

A conserved hypothetical gene is required but not sufficient for Ptr ToxC production in Pyrenophora tritici-repentis - (Peer Reviewed Journal)
Shi, G., Kariyawasam, G., Liu, S., Leng, Y., Zhong, S., Ali, S., Moolhuijzen, P., Moffat, C., Rasmussen, J., Friesen, T.L., Faris, J.D., Liu, Z. 2022. A conserved hypothetical gene is required but not sufficient for Ptr ToxC production in Pyrenophora tritici-repentis. Molecular Plant-Microbe Interactions. 35(4). https://apsjournals.apsnet.org/doi/10.1094/MPMI-12-21-0299-R.

Did the Parastagonospora nodorum necrotrophic effector SnTox3 evolved from SnTox5? - (Abstract Only)
Kariyawasam, G.K., Wyatt, N.A., Liu, Z., Stukenbrock, E.H., Faris, J.D., Friesen, T.L. 2022. Did the Parastagonospora nodorum necrotrophic effector SnTox3 evolved from SnTox5? [abstract]. Plant and Animal Genome Conference. Session No.7178.

Parastagonospora nodorum necrotrophic effector SnTox5 facilitates the colonization of leaf mesophyll in the presence and absence of Snn5 - (Abstract Only)
Kariyawasam, G.K., Richards, J.K., Wyatt, N.A., Running, K.L., Liu, Z., Faris, J.D., Friesen, T.L. 2022. Parastagonospora nodorum necrotrophic effector SnTox5 facilitates the colonization of leaf mesophyll in the presence and absence of Snn5 [abstract]. Plant and Animal Genome Conference. Session No.7511.

Characterization of the role of the Parastagonospora nodorum effector SnTox267 in virulence on wheat using confocal microscopy - (Abstract Only)
Nelson, A.C., Kariyawasam, G.K., Seneviratne, S., Borowicz, P., Faris, J.D., Liu, Z., Friesen, T.L. 2022. Characterization of the role of the Parastagonospora nodorum effector SnTox267 in virulence on wheat using confocal microscopy. In: Fungal Genetics Conference, March 15-20, 2022, Pacific Grove, CA.

The Parastagonospora nodorum necrotrophic effector SnTox3 likely evolved from a duplication event of SnTox5 - (Abstract Only)
Kariyawasam, G.K., Wyatt, N.A., Liu, Z., Stukenbrock, E.H., Faris, J.D., Friesen, T.L. 2022. The Parastagonospora nodorum necrotrophic effector SnTox3 likely evolved from a duplication event of SnTox5. In: Fungal Genetics Conference, Mar. 15-20,2022, Pacific Grove, CA.

Validation and characterization of Pyrenophora teres f. teres effectors VR1 and VR2 conferring virulence on Rika barley - (Abstract Only)
Li, J., Wyatt, N.A., Brueggeman, R.S., Friesen, T.L. 2022. Validation and characterization of Pyrenophora teres f. teres effectors VR1 and VR2 conferring virulence on Rika barley. In: Fungal Genetics Conference, Mar. 15-20,2022, Pacific Grove, CA.

Mutations in a barley cytochrome P450 gene enhances pathogen induced programmed cell death and cutin layer instability - (Peer Reviewed Journal)
Ameen, G., Solanki, S., Sager-Bittara, L., Richards, J., Tamang, P., Friesen, T.L., Brueggeman, R.S. 2021. Mutations in a barley cytochrome P450 gene enhances pathogen induced programmed cell death and cutin layer instability. PLoS Genetics. 17(12). Article e1009473. https://doi.org/10.1371/journal.pgen.1009473.

The Parastagonospora nodorum necrotrophic effector SnTox5 targets the wheat gene Snn5 and facilitates entry into the leaf mesophyll - (Pre-print Publication)
Kariyawasam, G.K., Richards, J.K., Wyatt, N.A., Running, K., Xu, S.S., Liu, Z., Borowicz, P., Faris, J.D., Friesen, T.L. 2021. The Parastagonospora nodorum necrotrophic effector SnTox5 targets the wheat gene Snn5 and facilitates entry into the leaf mesophyll. bioRxiv. https://doi.org/10.1101/2021.02.26.433117.

A triple threat: the Parastagonospora nodorum SnTox267 effector exploits three distinct host genetic factors to cause disease in wheat - (Pre-print Publication)
Richards, J.K., Kariyawasam, G.K., Seneviratne, S., Wyatt, N., Xu, S.S., Liu, Z., Faris, J.D., Friesen, T.L. 2021. A triple threat: the Parastagonospora nodorum SnTox267 effector exploits three distinct host genetic factors to cause disease in wheat. bioRxiv. https://doi.org/10.1101/2021.02.25.432871.

The Parastagonospora nodorum necrotrophic effector SnTox5 targets the wheat gene Snn5 and facilitates entry into the leaf mesophyll - (Peer Reviewed Journal)
Kariyawasam, G.K., Richards, J.K., Wyatt, N.A., Running, K., Xu, S.S., Liu, Z., Borowicz, P., Faris, J.D., Friesen, T.L. 2021. The Parastagonospora nodorum necrotrophic effector SnTox5 targets the wheat gene Snn5 and facilitates entry into the leaf mesophyll. New Phytologist. https://doi.org/10.1111/nph.17602.

A triple threat: The Parastagonospora nodorum SnTox267 effector exploits three distinct host genetic factors to cause disease in wheat - (Peer Reviewed Journal)
Richards, J.K., Seneviratne, S., Wyatt, N., Xu, S.S., Liu, Z., Faris, J.D., Friesen, T.L. 2021. A triple threat: The Parastagonospora nodorum SnTox267 effector exploits three distinct host genetic factors to cause disease in wheat. New Phytologist. https://doi.org/10.1111/nph.17601.

Characterization of effectors and their targets in necrotrophic pathogens reveals trends and variation in host manipulation - (Review Article)
Friesen, T.L., Faris, J.D. 2021. Characterization of effectors and their targets in necrotrophic pathogens reveals trends and variation in host manipulation. Annual Review of Phytopathology. https://doi.org/10.1146/annurev-phyto-120320-012807.

Development of a genome-wide genetic linkage map in the wheat pathogen Pyrenophora tritici-repentis and its utilization in genome assembling and comparison - (Peer Reviewed Journal)
Kariyawasam, G.K., Wyatt, N., Shi, G., Liu, S., Yan, C., Ma, Y., Zhong, S., Rasmussen, J.B., Moolhuijzen, P., Moffat, C.S., Friesen, T.L., Liu, Z. 2021. Development of a genome-wide genetic linkage map in the wheat pathogen Pyrenophora tritici-repentis and its utilization in genome assembling and comparison. Fungal Genetics and Biology. 152:103571. https://doi.org/10.1016/j.fgb.2021.103571.

Understanding the plant-pathogen interaction associated with septoria nodorum blotch of wheat - (Book / Chapter)

The barley HvWRKY6 transcription factor is required for resistance against Pyrenophora teres f. teres - (Peer Reviewed Journal)
Tamang, P., Richards, J.K., Solanki, S., Ameen, G., Poudel, R.S., Deka, P., Effertz, K., Clare, S., Hegstad, J., Bezbaruah, A.N., Li, X., Horsley, R.D., Friesen, T.L., Brueggeman, R.S. 2021. The barley HvWRKY6 transcription factor is required for resistance against Pyrenophora teres f. teres. Frontiers in Genetics. 11:601500. https://doi.org/10.3389/fgene.2020.601500.

Four reference quality genome assemblies of Pyrenophora teres f. maculata: A resource for studying the barley-spot form net blotch interaction - (Peer Reviewed Journal)
Wyatt, N., Friesen, T.L. 2021. Four reference quality genome assemblies of Pyrenophora teres f. maculata: A resource for studying the barley-spot form net blotch interaction. Molecular Plant-Microbe Interactions. 34:135-139. https://doi.org/10.1094/MPMI-08-20-0228-A.

Parastagonospora nodorum and related species in western Canada: genetic variability and effector genes - (Peer Reviewed Journal)
Hafez, M., Gourlie, R., Despins, T., Turkington, T.K., Friesen, T.L., Aboukhaddour, R. 2020. Parastagonospora nodorum and related species in western Canada: genetic variability and effector genes. Phytopathology. 110:1946-1958. https://doi.org/10.1094/PHYTO-05-20-0207-R.

Evidence of local adaptation and regional effector repertoires in Parastagonospora nodorum - (Abstract Only)
Richards, J.K., Stukenbrock, E.H., Liu, Z., Cowger, C., Faris, J.D., Friesen, T.L. 2020. Evidence of local adaptation and regional effector repertoires in Parastagonospora nodorum [abstract]. Plant and Animal Genome Conference. W502.

Research advances in the Pyrenophora teres-barley interaction - (Review Article)
Clare, S.J., Wyatt, N.A., Brueggeman, R.S., Friesen, T.L. 2020. Research advances in the Pyrenophora teres-barley interaction. Molecular Plant Pathology. 21(2):272-288. https://doi.org/10.1111/mpp.12896.

Validation of the P. teres f. teres effectors VR1 and VR2 conferring virulence on Rika barley identified in the bi-parental mapping population 15A × 6A - (Abstract Only)
Friesen, T.L., Wyatt, N.A. 2020. Validation of the P. teres f. teres effectors VR1 and VR2 conferring virulence on Rika barley identified in the bi-parental mapping population 15A × 6A. 15th European Conference on Fungal Genetics. Poster No.289.

Genome-wide association mapping identifies SnTox5 in Parastagonospora nodorum - (Abstract Only)

A comparative genomic analysis of the barley pathogen Pyrenophora teres f. teres identifies sub-telomeric regions as drivers of virulence - (Peer Reviewed Journal)
Wyatt, N.A., Richards, J.K., Brueggeman, R.S., Friesen, T.L. 2020. A comparative genomic analysis of the barley pathogen Pyrenophora teres f. teres identifies sub-telomeric regions as drivers of virulence. Molecular Plant-Microbe Interactions. 33:173-188. https://doi.org/10.1094/MPMI-05-19-0128-R.

Local adaptation drives the diversification of effectors in the fungal wheat pathogen Parastagonospora nodorum in the United States - (Peer Reviewed Journal)
Richards, J.K., Stukenbrock, E.H., Carpenter, J., Liu, Z., Cowger, C., Faris, J.D., Friesen, T.L. 2019. Local adaptation drives the diversification of effectors in the fungal wheat pathogen Parastagonospora nodorum in the United States. PLoS Genetics. 15(10):e1008223. https://doi.org/10.1371/journal.pgen.1008223.

Genome-wide association mapping identifies SnTox5 in Parastagonospora nodorum - (Abstract Only)

Functional characterization of the conserved effector AvrHar in Pyrenophora teres f. teres - (Abstract Only)

A necrotrophic effector from Parastagonospora nodorum triggers programmed cell death by targeting two distinct non-homoeologous wheat sensitivity genes - (Abstract Only)

Genome wide association in Parastagonospora nodorum identifies novel necrotrophic effectors with unique characteristics - (Abstract Only)

A single necrotrophic effector from Parastagonospora nodorum has two susceptibility targets in wheat - (Abstract Only)

Mapping of barley susceptibility/resistance QTL against spot form net blotch caused by Pyrenophora teres f. maculata using RIL populations - (Peer Reviewed Journal)
Tamang, P., Richards, J.K., Alhashal, A., Poudel, R.S., Horsley, R.D., Friesen, T.L., Brueggeman, R.S. 2019. Mapping of barley susceptibility/resistance QTL against spot form net blotch caused by Pyrenophora teres f. maculata using RIL populations. Theoretical and Applied Genetics. 132(7):1953-1963. https://doi.org/10.1007/s00122-019-03328-x.

Pan-parastagonospora comparative genome analysis - effector prediction and genome evolution - (Peer Reviewed Journal)
Syme, R.A., Tan, K., Rybak, K., Friesen, T.L., McDonald, B.A., Oliver, R.P., Hane, J.K. 2018. Pan-parastagonospora comparative genome analysis - effector prediction and genome evolution. Genome Biology and Evolution. 10(9):2443-2457. https://doi.org/10.1093/gbe/evy192.

ToxA is present in the U.S. Bipolaris sorokiniana population and is a significant virulence factor on wheat harboring Tsn1 - (Peer Reviewed Journal)
Friesen, T.L., Holmes, D.J., Bowden, R.L., Faris, J.D. 2018. ToxA is present in the U.S. Bipolaris sorokiniana population and is a significant virulence factor on wheat harboring Tsn1. Plant Disease. 102(12):2446-2452. Plant Dis. 102:2446-2452.

Transposable element genomic fissuring in Pyrenophora teres is associated with genome expansion and dynamics of host-pathogen genetic interactions - (Peer Reviewed Journal)
Syme, R.A., Martin, A., Wyatt, N.A., Lawrence, J.A., Muria-Gonzalez, M.J., Friesen, T.L., Ellwood, S.R. 2018. Transposable element genomic fissuring in Pyrenophora teres is associated with genome expansion and dynamics of host-pathogen genetic interactions. Frontiers in Genetics. 9:130. https://doi.org/10.3389/fgene.2018.00130.

Reference quality genome assemblies of three Parastagonospora nodorum isolates differing in virulence on wheat - (Peer Reviewed Journal)
Richards, J.K., Wyatt, N.A., Liu, Z., Faris, J.D., Friesen, T.L. 2018. Reference quality genome assemblies of three Parastagonospora nodorum isolates differing in virulence on wheat. G3, Genes/Genomes/Genetics. 8:393-399. https://doi.org/10.1534/g3.117.300462.

Reference assembly and annotation of the Pyrenophora teres f. teres isolate 0-1 - (Peer Reviewed Journal)
Wyatt, N.A., Brueggeman, R.S., Friesen, T.L. 2018. Reference assembly and annotation of the Pyrenophora teres f. teres isolate 0-1. G3, Genes/Genomes/Genetics. 8:1-8.

Molecular manipulation of the mating-type system and development of a new approach for characterizing pathogen virulence in Pyrenophora tritici-repentis - (Peer Reviewed Journal)
Ameen, G., Kariyawasam, G., Shi, G., Friesen, T.L., Faris, J.D., Ali, S., Rasmussen, J.B., Liu, Z. 2017. Molecular manipulation of the mating-type system and development of a new approach for characterizing pathogen virulence in Pyrenophora tritici-repentis. Fungal Genetics and Biology. 109:16-25. https://doi.org/10.1016/j.fgb.2017.10.004.

Genetic analysis of virulence in the Pyrenophora teres f. teres population BB25 x FGOH04Ptt021 - (Peer Reviewed Journal)
Koladia, V.M., Richards, J.K., Wyatt, N.A., Faris, J.D., Brueggeman, R.S., Friesen, T.L. 2017. Genetic analysis of virulence in the Pyrenophora teres f. teres population BB25 x FGOH04Ptt021. Fungal Genetics and Biology. 107:12-19. https://doi.org/10.1016/j.fgb.2017.07.003.

Mapping of SnTox3-Snn3 as a major determinant of field susceptibility to Septoria nodorum leaf blotch in the SHA3/CBRD x Naxos population - (Peer Reviewed Journal)
Ruud, A.K., Windju, S., Belova, T., Friesen, T.L., Lillemo, M. 2017. Mapping of SnTox3-Snn3 as a major determinant of field susceptibility to Septoria nodorum leaf blotch in the SHA3/CBRD x Naxos population. Theoretical and Applied Genetics. 130:1361-1374. doi: 10.1007/s00122-017-2893-5.