Research Project: Zoonotic Parasites Affecting Food Animals, Food Safety, and Public Health

Location: Environmental Microbial & Food Safety Laboratory

Publications (Clicking on the reprint icon will take you to the publication reprint.)

Detection of gastrointestinal protists - (Book / Chapter)
Santin, M, Ng-Hublin, J.S.Y, Maloney, J.G. 2024. In: Detection of gastrointestinal protists. Sulaiman, I.M. (Editor). Recent Advancements in the Diagnosis of Human Disease (1st Edition). CRC Press. pp 46-103. https://doi.org/10.1201/9781003438595

Investigation of neglected protists Blastocystis and Dientamoeba fragilis in immunocompetent and immunodeficient diarrheal patients using both conventional and molecular methods - (Peer Reviewed Journal)
Sarzhanov, F., Dogruman-All, F., Santin, M., Maloney, J.G., Gureser, A.S., Karasartova, D., Taylan-Ozkan, A. 2021. Investigation of neglected protists Blastocystis and Dientamoeba fragilis in immunocompetent and immunodeficient diarrheal patients using both conventional and molecular methods. PLOS Neglected Tropical Diseases. 15(10):e0009779. https://doi.org/10.1371/journal.pntd.0009779.

An Illumina MiSeq based amplicon sequencing method for the detection of mixed parasite infections using the Blastocystis SSU rRNA gene as an example - (Book / Chapter)
Maloney, J.G., George, N.S., Molokin, A., Santin, M. 2021. An Illumina MiSeq based amplicon sequencing method for the detection of mixed parasite infections using the Blastocystis SSU rRNA gene as an example. Methods in Molecular Biology. https://doi.org/10.1007/978-1-0716-1681-9_5.

Why foodborne and waterborne parasites are important for veterinarians - (Peer Reviewed Journal)
Almeria, S., Robertson, L., Santin, M. 2021. Why foodborne and waterborne parasites are important for veterinarians. Research in Veterinary Science. 11(3):700. https://doi.org/10.1016/j.rvsc.2021.02.020.

How to live in a dysbiosed environment: gut microbiota profiling in patients with co-occurrence of Blastocystis and Clostridioides difficile - (Peer Reviewed Journal)
Vega, L., Herrera, G., Muñoz, M., Patarroyo, M.A., Maloney, J.G., Santin, M., Ramirez, J.D. 2021. How to live in a dysbiosed environment: gut microbiota profiling in patients with co-occurrence of Blastocystis and Clostridioides difficile. Scientific Reports. https://doi.org/10.1371/journal.pone.0248185.

Use of Oxford Nanopore MinION to generate full-length sequences of the Blastocystis small subunit (SSU) rRNA gene - (Peer Reviewed Journal)
Maloney, J.G., Molokin, A., Santin, M. 2020. Use of Oxford Nanopore MinION to generate full-length sequences of the Blastocystis small subunit (SSU) rRNA gene. Parasites & Vectors. 13(1):595. https://doi.org/10.1186/s13071-020-04484-6.

Assessment of next generation amplicon sequencing of the beta-giardin gene for the detection of Giardia duodenalis assemblages and mixed infections - (Peer Reviewed Journal)
Maloney, J.G., Molokin, A., Santin, M. 2020. Assessment of next generation amplicon sequencing of the beta-giardin gene for the detection of Giardia duodenalis assemblages and mixed infections. Food and Waterborne Parasitology. 21:e00098. https://doi.org/10.1016/j.fawpar.2020.e00098.

Blastocystis in domesticated and wild mammals and birds - (Peer Reviewed Journal)
Hublin, J., Maloney, J.G., Santin, M. 2020. Blastocystis in domesticated and wild mammals and birds. Research in Veterinary Science. https://doi.org/10.1016/j.rvsc.2020.09.031.

Enterocytozoon bieneusi (Microsporidia): evidence of transmission between sympatric wild boars (Sus scrofa ferus) and Iberian pigs (Sus scrofa domesticus) in Southern Spain - (Peer Reviewed Journal)
Dashti, A., Rivero-Juarez, A., Santin, M., Lopez-Lopez, P., Caballero-Gomez, J., Frias-Casas, M., Koster, P., Bailo, B., Calero-Bernal, R., Briz, V., Carmena, D. 2020. Enterocytozoon bieneusi (Microsporidia): evidence of transmission between sympatric wild boars (Sus scrofa ferus) and Iberian pigs (Sus scrofa domesticus) in Southern Spain. Transboundary and Emerging Diseases. 67(6):2869-2880. https://doi.org/10.1111/tbed.13658.

First identification of genotypes of Enterocytozoon bieneusi (Microsporidia) among symptomatic and asymptomatic children in Mozambique - (Peer Reviewed Journal)
Salimo Muadica, A., Messa, A., Dashti, A., Santin, M., Manjate, F., Chirinda, P., Garrine, M., Vubil, D., Acacio, S., Koster, P., Bailo, B., Nhampossa, T., Calero-Bernal, R., Mwenda, J., Mandomando, I., Carmena, D. 2020. First identification of genotypes of Enterocytozoon bieneusi (Microsporidia) among symptomatic and asymptomatic children in Mozambique. PLOS Neglected Tropical Diseases. 14(6):e0008419. https://doi.org/10.1371/journal.pntd.0008419.

Differences in the microbial community and resistome structures of feces from pre-weaned calves and lactating dairy cows - (Peer Reviewed Journal)
Haley, B.J., Kim, S., Salaheen, S., Hovingh, E., Van Kessel, J.S. 2020. Differences in the microbial community and resistome structures of feces from pre-weaned calves and lactating dairy cows. Foodborne Pathogens and Disease. https://doi.org/10.1089/fpd.2019.2768.

Blastocystis subtype distribution in domestic and captive wild birds from Brazil using next generation amplicon sequencing - (Peer Reviewed Journal)
Maloney, J.G., Molokin, A., Cury, M.C., Santin, M., Da Cunha, M.J. 2020. Blastocystis subtype distribution in domestic and captive wild birds from Brazil using next generation amplicon sequencing. Parasite Epidemiology and Control. 9:e00138. https://doi.org/10.1016/j.parepi.2020.e00138.

Giardiasis and cryptoporidiosis - (Book / Chapter)
Santin, M. 2020. Giardiasis and cryptoporidiosis. Veterinary Clinics of North America. 36(1):223-238. https://doi.org/10.1016/j.cvfa.2019.11.005.

Use of next generation amplicon sequencing to study Blastocystis genetic diversity in a rural human population from Mexico - (Peer Reviewed Journal)
Rojas-Velazquez, L., Maloney, J.G., Molokin, A., Moran, P., Vazquez-Serrano, A., Gonzalez, E., Perez-Juarez, H., Ximenez, C., Santin, M. 2019. Use of next generation amplicon sequencing to study Blastocystis genetic diversity in a rural human population from Mexico. Parasites & Vectors. https://doi.org/10.1186/s13071-019-3814-z.

Blastocystis sp. subtype diversity in wild mesocarnivores from Spain - (Peer Reviewed Journal)
Calero-Bernal, R., Santin, M., Maloney, J.G., Martin-Perez, M., Habela, M., Fernandez-Garcia, J.L., Figueiredo, A., Najera, F., Palacios, M.J., Mateo, M., Balseiro, A., Barral, M., Lima Barbero, J., Koster, P.C., Carmena, D. 2019. Blastocystis sp. subtype diversity in wild mesocarnivores from Spain. Journal of Eukaryotic Microbiology. 67(2):273-278.

Occurrence and genetic diversity of Enterocytozoon bieneusi (Microsporidia) in owned and sheltered dogs and cats in Northern Spain - (Peer Reviewed Journal)
Dashti, A., Santin, M., Cano, L., De Lucio, A., Bailo, B., Hernandez De Mingo, M., Koster, P.C., Fernandez-Basterra, J.A., Aramburu-Aguirre, Lopez-Molina, N., Fernandez-Crespo, J.C., Calero-Bernal, R., Carmena, D. 2019. Occurrence and genetic diversity of Enterocytozoon bieneusi (Microsporidia) in owned and sheltered dogs and cats in Northern Spain. Parasitology Research. 118(10):2979-2987. https://doi.org/10.1007/s00436-019-06428-1.

Proliferation of resident macrophages is dispensable for protection during Giardia duodenalis infections - (Peer Reviewed Journal)
Fink, M.Y., Maloney, J.G., Keselman, A., Li, E., Menegas, S., Jones, C., Singer, S.M. 2019. Proliferation of resident macrophages is dispensable for protection during Giardia duodenalis infections. ImmunoHorizons. 3(8):412-421. https://doi.org/10.4049/immunohorizons.1900041.

Next generation amplicon sequencing improves detection of Blastocystis mixed subtype infections - (Peer Reviewed Journal)
Maloney, J.G., Molokin, A., Santin, M. 2019. Next generation amplicon sequencing improves detection of Blastocystis mixed subtype infections. Infection, Genetics and Evolution. 73:119-125. https://doi.org/10.1016/j.meegid.2019.04.013.

Host specificity in Enterocytozoon bieneusi and public health implications - (Peer Reviewed Journal)
Li, W., Santin, M., Feng, Y. 2019. Host specificity in Enterocytozoon bieneusi and public health implications. Trends in Parasitology. 35(6):436-451. https://doi.org/10.1016/j.pt.2019.04.004.

A highly sensitive method for detecting Cryptosporidium parvum oocysts recovered from source and finish water using RT-PCR directed to Cryspovirus RNA - (Peer Reviewed Journal)
Sato de Souza, M., O'Brien, C., Santin, M., Jenkins, M.C. 2018. A highly sensitive method for detecting Cryptosporidium parvum oocysts recovered from source and finish water using RT-PCR directed to Cryspovirus RNA. Journal of Microbiological Methods. 156:77-80. https://doi.org/10.1016/j.mimet.2018.11.022.

Zoonotic and genetically diverse subtypes of Blastocystis in U.S. dairy calves - (Peer Reviewed Journal)
Maloney, J., Lombard, J., Urie, N., Shivley, C., Santin, M. 2018. Zoonotic and genetically diverse subtypes of Blastocystis in U.S. dairy calves. Parasitology Research. 118(2):575-582. https://doi.org/10.1007/s00436-018-6149-3.

Cryptosporidium spp. CP15 and CSL protein-derived synthetic peptides - (Peer Reviewed Journal)
Avendano, C., Jenkins, M.C., Mendez-Callejas, G., Oviedo, J., Guzman, F., Patarroyo, M., Sanchez-Acedo, C., Quilez, J. 2018. Cryptosporidium spp. CP15 and CSL protein-derived synthetic peptides. Vaccine. 36(45):6703-6710. https://doi.org/10.1016/j.vaccine.2018.09.044.

Preweaned heifer management on US dairy operations: Part III. Factors associated with Cryptosporidium and Giardia in preweaned dairy heifer calves - (Peer Reviewed Journal)
Urie, N., Lombard, J., Shivley, C., Ashley, A., Kopral, C., Santin, M. 2018. Preweaned heifer management on US dairy operations: Part III. Factors associated with Cryptosporidium and Giardia in preweaned dairy heifer calves. Journal of Dairy Science. 101(10):9199-9213. https://doi.org/10.3168/jds.2017-14060.

Preweaned heifer management on US dairy operations: Part VI. Factors associated with average daily gain in preweaned dairy heifer calves - (Peer Reviewed Journal)
Shivley, C., Lombard, J., Urie, N., Kopral, C., Santin, M., Earleywine, T., Olson, J., Garry, F. 2018. Preweaned heifer management on US dairy operations: Part VI. Factors associated with average daily gain in preweaned dairy heifer calves. Journal of Dairy Science. 101(10):9245-9258. https://doi.org/10.3168/jds.2017-14022.

Molecular characterization of Cryptosporidium spp. in poultry from Brazil - (Peer Reviewed Journal)
Rodrigues Da Cunha, M.J., Cury, M.C., Santin-Duran, M. 2018. Molecular characterization of Cryptosporidium spp. in poultry from Brazil. Veterinary Parasitology. 118:331-335. https://doi.org/10.1016/j.rvsc.2018.03.010.

Molecular characterization of Enterocytozoon bieneusi in wild carnivores in Spain - (Peer Reviewed Journal)
Santin, M., Calero-Bernal, R., Carmena, D., Mateo, M., Balseiro, A., Barral, M., Limabarbero, J.F., Habela, M.A. 2017. Molecular characterization of Enterocytozoon bieneusi in wild carnivores in Spain. Journal of Eukaryotic Microbiology. 65(4):468-474. https://doi.org/10.1111/jeu.12492.

Interlaboratory validation of an improved U.S. Food and Drug Administration method for detection of Cyclospora cayetanensis in produce using TaqMan real-time PCR - (Peer Reviewed Journal)
Murphy, H.R., Cinar, H., Gopinath, G., Im, A., Noe, K.E., Chatman, L.D., Miranda, N.E., Wetherington, J.H., Neal-Mckinney, J., Piresc, G.S., Sachs, E., Stanya, K.J., Jognson, C.L., Nascimiento, F., Santin, M., Molokin, A., Samadpour, M., Janagama, H., Kahler, A., Miller, C., Da Silva, A.J. 2017. Interlaboratory validation of an improved U.S. Food and Drug Administration method for detection of Cyclospora cayetanensis in produce using TaqMan real-time PCR. International Journal of Food Microbiology. 69:170-178.

Molecular characterization of Enterocytozoon bieneusi in wild carnivores in Spain - (Abstract Only)
Santin, M., Calero-Bernal, R., Carmena, D., Mateo, M., Balseiro, A., Barral, M., Limabarbero, J.F., Habela, M.A. 2017. Molecular characterization of Enterocytozoon bieneusi in wild carnivores in Spain. Society of Protozoologists International Workshop on Opportunistic Protists. On August 11, 2017.

Blastocystis prevalence and subtype distribution among pre-weaned dairy calves in the United States - (Abstract Only)
Maloney, J.G., Santin, M. 2017. Blastocystis prevalence and subtype distribution among pre-weaned dairy calves in the United States. BARC Poster Day.

Molecular identification of Enterocytozoon bieneusi, Cryptosporidium, and Giardia in brazilian captive birds - (Peer Reviewed Journal)
Da Cunha, M.J., Cury, M.C., Santin, M. 2017. Molecular identification of Enterocytozoon bieneusi, Cryptosporidium, and Giardia in brazilian captive birds. Parasitology Research. 116:487-493. https://doi.org/10.1007/s00436-016-5309-6.

Zoonotic Enterocytozoon bieneusi Genotypes found in Brazilian sheep - (Peer Reviewed Journal)
Fiuza, V., Lopes, C., Cosendey, R., Oliviera, F., Fayer, R., Santin, M. 2016. Zoonotic Enterocytozoon bieneusi Genotypes found in Brazilian sheep. Research in Veterinary Science. 107:196-201.

Effects of Enterococcus faecalis CECT 7121 on Cryptosporidium parvum infection in mice - (Peer Reviewed Journal)
Del Coco, V.F., Sparo, M.D., Sidoti, A., Santin, M., Basualdo, J.A., Codorba, M.A. 2016. Effects of Enterococcus faecalis CECT 7121 on Cryptosporidium parvum infection in mice. Parasitology Research. 115(8):3239-3244. https://doi.org/10.1007/s00436-016-5087-1.