Location: Food Animal Metabolism Research
2017 Annual Report
Accomplishments
1. Rapid screening test developed. Zilpaterol is an FDA-approved beta-agonist feed additive that increases feed efficiency, improves growth rate, and produces lean meat in livestock. Several major trade partners with the United States do not allow the import of meat from animals fed zilpaterol and in the U.S. zilpaterol is illegal to use in some food animal species. ARS researchers in Fargo, North Dakota developed a sensitive, selective, inexpensive, and rapid test to determine whether animals have been exposed to zilpaterol. The assay, which is similar to an over-the-counter pregnancy test, can be used on-site with minimal training, and results are obtained in about 10 minutes. The accuracy and sensitivity of the assay was verified in tissues and urine from animals exposed to zilpaterol. This simple and inexpensive assay could be used to determine accidental, illegal, or purposeful zilpaterol exposure.
Review Publications
Cheng, T.D., Shelver, W.L., Hong, C., McCann, S.E., Davis, W., Zhang, Y., Ambrosone, C.B., Smith, D.J. 2016. Urinary excretion of the ß-adrenergic feed additives ractopamine and zilpaterol in breast and lung cancer patients. Journal of Agricultural and Food Chemistry. 64(40):7632-7639.
Smith, D.J., Giddings, J.M., Herges, G.R., Ernst, W. 2016. Distribution, identification, and quantification of residues after treatment of ready-to-eat salami with 36Cl-labeled or nonlabeled chlorine dioxide gas. Journal of Agricultural and Food Chemistry. 64:8454-8462.
Smith, D.J., Shelver, W.L., Marx, A. 2016. Detection of residues in urine and tissues of sheep treated with trace levels of dietary ractopamine HCl. Journal of Animal Science. 94:5423–5433. doi:10.2527/jas2016-0899.
Shelver, W.L., Schneider, M.J., Smith, D.J. 2016. Distribution of flunixin residues in muscles of dairy cattle dosed with lipopolysaccharide or saline and treated with flunixin by intravenous or intramuscular injection. Journal of Agricultural and Food Chemistry. 64:9697-9701. doi:10.1021/acs.jafc.6b04792.
Shelver, W.L., Chakrabarty, S., Smith, D.J. 2017. Comparison of lateral flow assay, kidney inhibition swab, and liquid chromatography-tandem mass spectrometry for the detection of penicillin G residues in sow urine. Journal of Agricultural and Food Chemistry. 65:1778-1783. doi:10.1021/acs.jafc.6b05049.
Shappell, N.W., Shelver, W.L., Lupton, S.J., Fanaselle, W., Van Doren, J.M., Hakk, H. 2017. Distribution of animal drugs among curd, whey, and milk protein fractions in spiked skim milk and whey. Journal of Agricultural and Food Chemistry. 65:938-949. doi:10.1021/acs.jafc.6b04258.
Smith, D.J., Herges, G.R. 2017. Stability of sodium chlorate residues in frozen tomato and cantaloupe homogenates. Journal of Agricultural and Food Chemistry. 65(30):6258-6263. doi:10.1021/acs.jafc.7b02520.
Smith, D.J., Kim, M. 2017. Chemical contamination of red meat. In: Schrenk, D., editor. Chemical Contaminants and Residues in Food, 2nd edition. Woodhead Publishing, Oxford. p.451-489.
Casey, F.X., Shappell, N.W., Hakk, H. 2017. Halogenated 17ß-estradiol surrogates: synthesis, estrogenic activity, and initial investigations of fate in soil/water systems. Journal of Environmental Quality. 46(4):802-810. doi: 10.2134/jeq2017.02.0053.
