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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Food Safety and Enteric Pathogens Research » Research » Research Project #430611
Research Project: Characterization of Colonization of Shiga Toxin-producing Escherichia coli (STEC) in Cattle and Strategies for Effective Preharvest Control

Location: Food Safety and Enteric Pathogens Research

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)

Novel reusable animal model for comparative evaluation of in vivo growth and protein-expression of Escherichia coli O157 strains in the bovine rumen Reprint Icon - (Peer Reviewed Journal)
Kudva, I.T., Trachsel, J.M., Biernbaum, E.N., Casey, T. 2022. Novel reusable animal model for comparative evaluation of in vivo growth and protein-expression of Escherichia coli O157 strains in the bovine rumen. PLoS ONE. 17(5). Article e0268645. https://doi.org/10.1371/journal.pone.0268645.

Genomewide transcriptional response of Escherichia coli O157:H7 to norepinephrine Reprint Icon - (Peer Reviewed Journal)
Sharma, V.K., Akavaram, S.N., Bayles, D.O. 2022. Genomewide transcriptional response of Escherichia coli O157:H7 to norepinephrine. BMC Genomics. 23. Article 107. https://doi.org/10.1186/s12864-021-08167-z.

EDL933 Strains of Escherichia coli O157 can demonstrate differential adherence to bovine recto-anal junction squamous epithelial cells Reprint Icon - (Peer Reviewed Journal)
Hoeffler, R.S., Kudva, I.T. 2021. EDL933 Strains of Escherichia coli O157 can demonstrate differential adherence to bovine recto-anal junction squamous epithelial cells. Letters in Applied Microbiology. 15(1):129-138. https://doi.org/10.2174/1874285802115010129.

Strain and host-cell type dependent role of type 1 fimbriae genes in the adherence phenotype of super-shedder strains of Escherichia coli O157:H7 Reprint Icon - (Peer Reviewed Journal)
Katani, R., Kudva, I.T., Srinivasan, S., Schilling, M., Cote, R., Li, L., DebRoy, C., Arthur, T.M., Kapur, V., Stasko, J.A. 2021. Strain and host-cell type dependent role of type 1 fimbriae genes in the adherence phenotype of super-shedder strains of Escherichia coli O157:H7. International Journal of Medical Microbiology. 311(4). https://doi.org/10.1016/j.ijmm.2021.151511.

Mucosal IFN-¿ production and potential role in protection in Escherichia coli O157:H7 vaccinated and challenged cattle Reprint Icon - (Peer Reviewed Journal)
Schaut, R.G., Palmer, M.V., Boggiatto, P.M., Kudva, I.T., Loving, C.L., Sharma, V.K. 2021. Mucosal IFN-¿ production and potential role in protection in Escherichia coli O157:H7 vaccinated and challenged cattle. Infection and Immunity. 11. Article 9769. https://doi.org/10.1038/s41598-021-89113-7.

Evaluation of cattle for naturally colonized Shiga toxin-producing Escherichia coli requires combinatorial strategies Reprint Icon - (Peer Reviewed Journal)
Kudva, I.T., Oosthuysen, E.R., Wheeler, B.K., Loest, C.A. 2021. Evaluation of cattle for naturally colonized Shiga toxin-producing Escherichia coli requires combinatorial strategies. International Journal of Microbiology. 2021. Article 6673202. https://doi.org/10.1155/2021/6673202.

Non-fimbrial adhesin mutants reveal divergent Escherichia coli O157:H7 adherence mechanisms on human and cattle epithelial cells Reprint Icon - (Peer Reviewed Journal)
Moreau, M.R., Kudva, I.T., Katani, R., Cote, R., Li, L., Arthur, T.M., Kapur, V. 2021. Non-fimbrial adhesin mutants reveal divergent Escherichia coli O157:H7 adherence mechanisms on human and cattle epithelial cells. International Journal of Microbiology. 2021. Article 8868151. https://doi.org/10.1155/2021/8868151.

Recto-anal junction (RAJ) fecal microbiomes of cattle experimentally challenged with Escherichia coli O157:H7 Reprint Icon - (Peer Reviewed Journal)
Mir, R., Schaut, R.G., Looft, T.P., Allen, H.K., Sharma, V.K., Kudva, I.T. 2020. Recto-anal junction (RAJ) fecal microbiomes of cattle experimentally challenged with Escherichia coli O157:H7. Frontiers in Microbiology. 11:693. https://doi.org/10.3389/fmicb.2020.00693.

Super-shed Escherichia coli O157:H7 have potential for increased persistence on the recto-anal junction squamous epithelial cells and antibiotic resistance Reprint Icon - (Peer Reviewed Journal)
Mir, R.A., Brunelle, B.W., Alt, D.P., Arthur, T.M., Kudva, I.T. 2020. Super-shed Escherichia coli O157:H7 has potential for increased persistence on the recto-anal junction squamous epithelial cells and antibiotic resistance. International Journal of Microbiology. 2020(2368154):1-16. https://doi.org/10.1155/2020/2368154.

Cattle intestinal microbiota shifts following Escherichia coli O157:H7 vaccination and colonization Reprint Icon - (Peer Reviewed Journal)
Mir, R.A., Schaut, R.A., Allen, H.K., Looft, T.P., Loving, C.L., Kudva, I.T., Sharma, V.K. 2019. Cattle intestinal microbiota shifts following Escherichia coli O157:H7 vaccination and colonization. PLoS One. 14(12):e0226099. https://doi.org/10.1371/journal.pone.0226099.

The Escherichia coli O157:H7 carbon starvation-inducible lipoprotein Slp contributes to initial adherence in vitro via the human polymeric immunoglobulin receptor Reprint Icon - (Peer Reviewed Journal)
Fedorchuk, C., Kudva, I.T., Kariyawasam, S. 2019. The Escherichia coli O157:H7 carbon starvation-inducible lipoprotein Slp contributes to initial adherence in vitro via the human polymeric immunoglobulin receptor. PLoS One. 14(6):e0216791. https://doi.org/10.1371/journal.pone.0216791.

Cellular and mucosal immune responses following vaccination with inactivated mutant of Escherichia coli O157:H7 Reprint Icon - (Peer Reviewed Journal)
Schaut, R.G., Boggiatto, P.M., Loving, C.L., Sharma, V.K. 2019. Cellular and mucosal immune responses following vaccination with inactivated mutant of Escherichia coli O157:H7. Scientific Reports. 9:6401. https://doi.org/10.1038/s41598-019-42861-z.

The big six non-O157 Shiga toxin producing Escherichia coli demonstrate differential growth patterns, in vitro and in vivo, in rumen fluid of cattle - (Abstract Only)

Comparative genomics reveals structural and functional features specific to the genome of a foodborne Escherichia coli O157:H7 Reprint Icon - (Peer Reviewed Journal)
Sharma, V.K., Akavaram, S.N., Schaut, R.G., Bayles, D.O. 2019. Comparative genomics reveals structural and functional features specific to the genome of a foodborne Escherichia coli O157:H7. BMC Genomics. 20(1). Article 196. https://doi.org/10.1186/s12864-019-5568-6.

Comparative genomics reveals structural and functional features specific to the genome of a foodborne Escherichia coli O157:H7 Reprint Icon - (Peer Reviewed Journal)
Sharma, V.K., Akavaram, S.N., Schaut, R.G., Bayles, D.O. 2019. Comparative genomics reveals structural and functional features specific to the genome of a foodborne Escherichia coli O157:H7. BMC Genomics. 20:196. https://doi.org/10.1186/s12864-019-5568-6.

Fecal microbiota changes associated with dehorning and castration stress primarily affects light-weight dairy calves Reprint Icon - (Peer Reviewed Journal)
Mir, R.A., Kleinhenz, M.D., Coetzee, J.F., Allen, H.K., Kudva, I.T. 2019. Fecal microbiota changes associated with dehorning and castration stress primarily affects light-weight dairy calves. PLoS One. 14(1):e0210203. https://doi.org/10.1371/journal.pone.0210203.

Antibiotic resistant Shiga toxin-producing Escherichia coli: An overview of prevalence and intervention strategies Reprint Icon - (Review Article)
Mir, R.A., Kudva, I.T. 2019. Antibiotic resistant Shiga toxin-producing Escherichia coli: An overview of prevalence and intervention strategies. Zoonoses and Public Health. 66:1-13. https://doi.org/10.1111/zph.12533.

Vaccination with killed whole-cells of Escherichia coli O157:H7 hha mutant emulsified with an adjuvant induced vaccine strain-specific serum antibodies and reduced E. coli O157:H7 fecal shedding in cattle Reprint Icon - (Peer Reviewed Journal)
Sharma, V.K., Schaut, R.G., Loving, C.L. 2018. Vaccination with killed whole-cells of Escherichia coli O157:H7 hha mutant emulsified with an adjuvant induced vaccine strain-specific serum antibodies and reduced E. coli O157:H7 fecal shedding in cattle. Veterinary Microbiology. 219:190-199. https://doi.10.1016/j.vetmic.2018.04.003.

Escherichia coli O157:H7 virulence factors differentially impact cattle and bison macrophage killing Reprint Icon - (Peer Reviewed Journal)
Schaut, R.G., Loving, C.L., Sharma, V.K. 2018. Escherichia coli O157:H7 virulence factors differentially impact cattle and bison macrophage killing capacity. Microbial Pathogenesis. 118:251-256. https://doi.org/10.1016/j.micpath.2018.03.045.

Antibiotic susceptibility-resistance profiles of super-shed Escherichia coli O157:H7 - (Abstract Only)

Recto-anal junction (RAJ) microbiota composition in Escherichia coli O157:H7 shedding cattle - (Abstract Only)

Microbiota response to Escherichia coli O157:H7 colonization in cattle - (Abstract Only)

Comparative sequence analysis revealed altered chromosomal organization and a novel insertion sequence encoding DNA modification and potentially stress-related functions in an Escherichia coli O157:H7 foodborne isolate - (Abstract Only)

Escherichia coli O157:H7 and rectoanal junction cell interactome - (Abstract Only)

FimH has a strain and host-cell type dependent role in adherence of E. coli O157:H7 super-shedder strains to host cells - (Abstract Only)

Evaluation of an inactivated whole-cell vaccine-adjuvant preparation for reducing fecal shedding of Escherichia coli O157:H7 in cattle - (Abstract Only)
Sharma, V.K. 2017. Evaluation of an inactivated whole-cell vaccine-adjuvant preparation for reducing fecal shedding of Escherichia coli O157:H7 in cattle [abstract]. Microbe, American Society of Microbiology, June 1-5, 2017, New Orleans, Louisiana. Page No. 669.

Adverse effects of stress on microbiota - (Abstract Only)

Disruption of rcsB by a duplicated sequence in a curli-producing Escherichia coli O157:H7 results in differential gene expression in relation to biofilm formation, stress responses, and metabolism - (Peer Reviewed Journal)
Sharma, V.K., Bayles, D.O., Alt, D.P., Looft, T.P., Brunelle, B.W., Stasko, J.A. 2017. Disruption of rcsB by a duplicated sequence in a curli-producing Escherichia coli O157:H7 results in differential gene expression in relation to biofilm formation, stress responses, and metabolism. BMC Microbiology. 17(1):56. doi: 10.1186/s12866-017-0966-x.

Super-Shed Escherichia coli O157:H7 have potential for increased pathogen persistence and antibiotic resistance dissemination - (Abstract Only)

Complete genome sequences of curli-negative and curli-positive isolates of foodborne Escherichia coli O157:H7 strain 86-24 - (Peer Reviewed Journal)
Sharma, V.K., Bayles, D.O., Alt, D.P., Looft, T.P. 2016. Complete genome sequences of curli-negative and curli-positive isolates of foodborne Escherichia coli O157:H7 strain 86-24. Genome Announcements. 4(6):e01323-16. doi: 10.1128/genomeA.01323-16.

A tandem duplication of a 5-bp sequence in the rcsB gene confers biofilm-producing phenotype in Escherichia coli O157:H7 strain 86-24 - (Abstract Only)

Targeting reservoirs to control human infections – a one health approach - (Abstract Only)