Location: Crop Diseases, Pests and Genetics Research
Project Number: 2034-22000-013-006-T
Project Type: Trust Fund Cooperative Agreement
Start Date: Jan 1, 2017
End Date: Feb 25, 2020
Objective:
The research objective is to improve reliable diagnosis of "Candidatus Liberibacter asiaticus", presumptive causal agent of huanglongbing (HLB)in the U.S.
Approach:
The approach includes deep sequencing using MiSeq Illumina to improve full length sequence data of 'Ca. Liberibacter asiaticus'; and bioinformatic analysis, employing primer design software, Taqman probes, real time quantitative PCR, droplet digital PCR, high throughput robotic DNA extraction and purification of nucleic acid.
