Location: Grape Genetics Research Unit (GGRU)
Project Number: 8060-21220-007-005-R
Project Type: Reimbursable Cooperative Agreement
Start Date: Oct 1, 2016
End Date: Aug 31, 2021
Objective:
Determine the physiological and transcriptomic capacity for grapevine rootstocks to impact scion phenotypes. ARS Geneva will function as a central resource for RNA isolation and sequencing for the project as well as host a replicated rootstock mapping population vineyard. Studies will be conducted in 4 aims. Aim 1 will examine rootstock genotype effects and the interaction of irrigation treatment on Chambourcin scions. Aim 2 will examine effects of differing climatic conditions in established California vineyards with two rootstocks x two scions (Chardonnay and Cabernet Sauvignon). Aim 3 will examine the combined effects of variable rootstock genotype and variable climate by examining a rootstock mapping population planted out in 4 different, replicated vineyards. Sites include NY, SD, MO, and CA. Aim 4 will be broader aims targeting student training and outreach with stakeholders in the four mentioned states.
Approach:
The research proposed is conducted in three research aims 1) evaluate rootstock effects on scion phenotypes and transcriptomic effect for 4 different rootstocks in a research vineyard in Missouri; 2) evaluate the phenotypic and transcriptomic interaction effects of 2 different commercial rootstocks (1103P and 3309C) on 2 different scions (Chardonnay and Cabernet Sauvignon) in 8 different vineyards in California; and 3) examine the effect of rootstock genotype on scion phenotype in a replicated mapping population planted in 4 geographic sites. ARS’s role in the project will be to serve as a central RNA extraction and library preparation center as well as a site for one of the 4 mapping population vineyards. Extensive interaction is proposed for phenotyping and bioinformatics studies in collaboration with Saint Louis University, Missouri State University, University of Missouri, the Donald Danforth Plant Science Center and South Dakota State University.
USDA will participate by acting as a centralized RNA processing and isolation site, leveraging onsite expertise of SYs. USDA will also host one of the four proposed experimental vineyards for a rootstock mapping population. As such, maintainance and phenotyping will be conducted on site in tandem with RNA isolation and gene epression studies. Collaborative interaction with the above mentioned institutions will be used to integrate phenotyping, genotyping, and bioinformatic analysis of the proposed experiments.
In years 1-3 of the project, an ORISE student or GS-5 technician will: 1) Receive shipped grapevine tissue samples from the cooperating Universities and perform RNA extraction and library preparation. Samples will be shipped to a sequencing center (e.g. Cornell University) for sequencing. In years 3-5 of the project, an ORISE student or GS-5 technician will conduct the above tasks. In years 4-5 of the project, and additional GS-5 technician will assist in higher throughput RNA isolation and library preparation.
