Research Project: Characterization of the Pathogenesis and Antigen Expression in Spirochete Diseases

Location: Infectious Bacterial Diseases Research

Publications (Clicking on the reprint icon will take you to the publication reprint.)

Divergent lineages of pathogenic Leptospira species are widespread and persisting in the environment in Puerto Rico, USA - (Peer Reviewed Journal)
Stone, N.E., Hall, C.M., Ortiz, M., Hutton, S., Santana-Popper, E., Celona, K.R., Williamson, C.H., Bratsch, N., Fernandes, L.G., Busch, J.D., Pearson, T., Rivera-Garcia, S., Soltero, F., Galloway, R., Weiner, Z., Hoffmaster, A.R., Sahl, J.E., Nally, J.E., Wagner, D.M. 2022. Divergent lineages of pathogenic Leptospira species are widespread and persisting in the environment in Puerto Rico, USA. PLOS Neglected Tropical Diseases. 16(5). e0009959. https://doi.org/10.1371/journal.pntd.0009959.

Assessing rodents as carriers of pathogenic leptospires in the U.S. Virgin Islands: the risk to animal and public health - (Peer Reviewed Journal)
Hamond, C., Browne, A.S., Hornsby, R.L., Lecount, K., Anderson, T., Stuber, T., De Wilde, L.H., Cranford, H.M., Browne, S.K., Blanchard, G., Horner, D., Taylor, M.L., Evans, M., Roth, J., Bisgard, K., Salzer, J., Schafer, I., Ellis, B., Alt, D.P., Schlater, L., Nally, J.E., Ellis, E.M. 2022. Assessing rodents as carriers of pathogenic leptospires in the U.S. Virgin Islands: the risk to animal and public health. Scientific Reports. 12. Article 1132. https://doi.org/10.1038/s41598-022-04846-3.

Lesion material from Treponema-associated Hoof Disease of wild elk induces disease pathology in the sheep Digital Dermatitis model - (Peer Reviewed Journal)
Wilson-Welder, J.H., Olsen, S.C., Alt, D.P., Bayles, D.O., Han, S., Mansfield, K. 2022. Lesion material from Treponema-associated Hoof Disease of wild elk induces disease pathology in the sheep Digital Dermatitis model. Frontiers in Veterinary Science. 8. Article 782149. https://doi.org/10.3389/fvets.2021.782149.

Circulating foamy macrophages in the golden syrian hamster (mesocricetus auratus) model of Leptospirosis - (Peer Reviewed Journal)
Putz, E.J., Andreasen, C.B., Stasko, J.A., Fernandes, L.G., Palmer, M.V., Rauh, M.J., Nally, J.E. 2021. Circulating foamy macrophages in the golden syrian hamster (mesocricetus auratus) model of Leptospirosis. Journal of Comparative Pathology. 189(10):98-109. https://doi.org/10.1016/j.jcpa.2021.10.004.

Mongooses (Urva auropunctata) as reservoir hosts of Leptospira species in the United States Virgin Islands, 2019–2020 - (Peer Reviewed Journal)
Cranford, H.M., Browne, A.S., Lecount, K., Anderson, T., Hamond, C., Schlater, L., Stuber, T., Burke-France, V.J., Taylor, M., Harrison, C.J., Matias, K.Y., Medley, A., Rossow, J., Wiese, N., Jankelunas, L., De Wilde, L., Mehalick, M., Blanchard, G.L., Garcia, K.R., Mckinley, A.S., Lombard, C.D., Valiulis, J., Bradford, B., Berentsen, A., Salzer, J.S., Galloway, R., Schafer, I.J., Bisgard, K., Roth, J., Ellis, B.R., Ellis, E.M., Nally, J.E. 2021. Mongooses (Urva auropunctata) as reservoir hosts of Leptospira species in the United States Virgin Islands, 2019–2020. PLOS Neglected Tropical Diseases. 15(11). Article e0009859. https://doi.org/10.1371/journal.pntd.0009859.

Antigen specific urinary immunoglobulin in reservoir hosts of leptospirosis - (Peer Reviewed Journal)
Nally, J.E., Hornsby, R.L., Alt, D.P. 2021. Antigen specific urinary immunoglobulin in reservoir hosts of leptospirosis. Veterinary Sciences. 8(9). Article 178. https://doi.org/10.3390/vetsci8090178.

Characterization of bovine endometrial epithelial cell inflammatory response to Leptospira borgpetersenii serovar Hardjo - (Peer Reviewed Journal)
Molinari, P.C., Nally, J.E., Bromfield, J.L. 2021. Characterization of bovine endometrial epithelial cell inflammatory response to Leptospira borgpetersenii serovar Hardjo. Veterinary Immunology and Immunopathology. vol.2 issue 3 pgs 187-196. https://doi.org/10.1530/RAF-21-0012.

Application of CRISPR interference (CRISPRi) for gene silencing in pathogenic species of Leptospira - (Peer Reviewed Journal)
Fernandes, L.G., Hornsby, R.L., Nascimento, A., Nally, J.E. 2021. Application of CRISPR interference (CRISPRi) for gene silencing in pathogenic species of Leptospira. The Journal of Visualized Experiments (JoVE). 174. Article e62631. https://doi.org/10.3791/62631.

Prevalence of pathogenic Leptospira in livestock in St. Croix, U.S. Virgin Islands - (Peer Reviewed Journal)
Cranford, H.M., Taylor, M., Browne, A., Alt, D.P., Anderson, T., Hammond, C., Hornsby, R.L., Lecount, K., Schlater, L., Stuber, T., Dewilde, L., Burke-France, V.J., Ellis, E.M., Nally, J.E., Bradford, B. 2021. Prevalence of pathogenic Leptospira in livestock in St. Croix, U.S. Virgin Islands. Tropical Medicine and Infectious Disease. 6(2), Article 85. https://doi.org/10.3390/tropicalmed6020085.

Use of food enrichment for medication delivery in large groups of sheep - (Trade Journal)
Wilson-Welder, J.H., Frank, A.T. 2021. Use of food enrichment for medication delivery in large groups of sheep. Journal of the American Association for Laboratory Animal Science. p. 40-43.

Distinct transcriptional profiles of Leptospira borgpetersenii serovar Hardjo strains JB197 and HB203 cultured at different temperatures - (Peer Reviewed Journal)
Putz, E.J., Sivasankaran, S.K., Fernandes, L.G., Brunelle, B.W., Lippolis, J.D., Alt, D.P., Bayles, D.O., Hornsby, R.L., Nally, J.E. 2021. Distinct transcriptional profiles of Leptospira borgpetersenii serovar Hardjo strains JB197 and HB203 cultured at different temperatures. PLOS Neglected Tropical Diseases. 15(4). Article e0009320. https://doi.org/10.1371/journal.pntd.0009320.

Bovine immune response to vaccination and infection with Leptospira borgpetersenii serovar Hardjo - (Peer Reviewed Journal)
Wilson-Welder, J.H., Alt, D.P., Nally, J.E., Olsen, S.C. 2021. Bovine immune response to vaccination and infection with Leptospira borgpetersenii serovar Hardjo. mSphere. 6(2). https://doi.org/10.1128/mSphere.00988-20.

Qualitative and semiquantitative assessment of thyroid hormone binding proteins in greyhounds and other dog breeds - (Peer Reviewed Journal)
Shiel, R.E., Nolan, C.M., Nally, J.E., Refsal, K.R., Mooney, C.T. 2021. Qualitative and semiquantitative assessment of thyroid hormone binding proteins in greyhounds and other dog breeds. Domestic Animal Endocrinology. 76:1-8. https://doi.org/10.1016/j.domaniend.2021.106623.

Genetic manipulation of pathogenic Leptospira: CRISPR interference (CRISPRi)-mediated gene silencing and rapid mutant recovery at 37C - (Peer Reviewed Journal)
Fernandes, L.G., Hornsby, R.L., Nascimento, A.L., Nally, J.E. 2021. Genetic manipulation of pathogenic Leptospira: CRISPR interference (CRISPRi)-mediated gene silencing and rapid mutant recovery at 37C. Scientific Reports. 11. https://doi.org/10.1038/s41598-021-81400-7.

A live attenuated-vaccine model confers cross-protective immunity against different species of the Leptospira genus - (Peer Reviewed Journal)
Wunder, E.A., Adhikarla, H., Hamond, C., Owers, K., Liang, L., Rodrigues, C.B., Bisht, V., Nally, J.E., Alt, D.P., Reis, M.G., Diggle, P.J., Felgner, P.L., Ko, A.I. 2021. A live attenuated-vaccine model confers cross-protective immunity against different species of the Leptospira genus. eLife. 10:e64166. https://doi.org/10.7554/eLife.64166.

Investigating the immunological and biological equilibrium of reservoir hosts and pathogenic Leptospira: balancing the solution to an acute problem - (Peer Reviewed Journal)
Putz, E.J., Nally, J.E. 2020. Investigating the immunological and biological equilibrium of reservoir hosts and pathogenic Leptospira: balancing the solution to an acute problem. Frontiers in Immunology. 11:2005. https://doi.org/10.3389/fmicb.2020.02005.

Isolation and propagation of leptospires at 37C directly from the mammalian host - (Peer Reviewed Journal)
Hornsby, R.L., Alt, D.P., Nally, J.E. 2020. Isolation and propagation of leptospires at 37 degree C directly from the mammalian host. Scientific Reports. 10(9620). https://doi.org/10.1038/s41598-020-66526-4.

Comparison of real-time PCR, bacteriologic culture and fluorescent antibody test for the detection of Leptospira borgpetersenii in urine of naturally infected cattle - (Peer Reviewed Journal)
Nally, J.E., Ahmed, A., Putz, E.J., Palmquist, D.E., Goris, M. 2020. Comparison of real-time PCR, bacteriologic culture and fluorescent antibody test for the detection of Leptospira borgpetersenii in urine of naturally infected cattle. Veterinary Sciences. 7(2):66. https://doi.org/10.3390/vetsci7020066.

Bovine immune response to leptospiral antigen in different novel adjuvants and vaccine delivery platforms - (Peer Reviewed Journal)
Wilson-Welder, J.H., Boggiatto, P.M., Nally, J.E., Wafa, E.I., Alt, D.P., Hornsby, R.L., Frank, A.T., Jones, D.E., Olsen, S.C., Bowden, N.B., Salem, A.K. 2020. Bovine immune response to leptospiral antigen in different novel adjuvants and vaccine delivery platforms. Vaccine. 38(18):3464-3473. https://doi.org/10.1016/j.vaccine.2020.02.086.

Evaluation of protective and immune responses following vaccination with recombinant MIP and CPAF from Chlamydia abortus as novel vaccines for enzootic abortion of ewes - (Peer Reviewed Journal)
O'Neill, L., Keane, O.M., Ross, P.J., Nally, J.E., Seshu, J., Markey, B.K. 2019. Evaluation of protective and immune responses following vaccination with recombinant MIP and CPAF from Chlamydia abortus as novel vaccines for enzootic abortion of ewes. Vaccine. 37(36):5428-5438. https://doi.org/10.1016/j.vaccine.2019.06.088.

Phenotypic and proteomic characterization of treponemes associated with bovine digital dermatitis - (Peer Reviewed Journal)
Nally, J.E., Hornsby, R.L., Alt, D.P., Whitelegge, J.P. 2019. Phenotypic and proteomic characterization of treponemes associated with bovine digital dermatitis. Veterinary Microbiology. 235:35-42. https://doi.org/10.1016/j.vetmic.2019.05.023.

Further inquiry into the etiology of hoof disease in elk using bacterial metagenomics analysis - (Abstract Only)

Dairy science and health in the tropics: challenges and opportunities for the next decades - (Peer Reviewed Journal)
Hernandex-Castellano, L.E., Nally, J.E., Lindahl, J., Wanapat, M., Alhidary, I.A., Fangueiro, D., Grace, D., Ratto, M., Bambou, J.C., de Almeida, A.M. 2019. Dairy science and health in the tropics: challenges and opportunities for the next decades. Tropical Animal Health and Production. 51(5):1009-1017. https://doi.org/10.1007/s11250-019-01866-6.

Macrophages and galectin 3 control bacterial burden in acute and subacute murine leptospirosis that determines chronic kidney fibrosis - (Peer Reviewed Journal)
Ferrer, M.F., Scharrig, E., Charo, N., Ripodas, A.L., Drut, R., Carrera Silva, E.A., Nagel, A., Nally, J.E., Schattner, M., Gomez, R.M., Montes de Oca, D.P. 2018. Macrophages and galectin 3 control bacterial burden in acute and subacute murine leptospirosis that determines chronic kidney fibrosis. Frontiers in Cellular and Infection Microbiology. 8:384. https://doi.org/10.3389/fcimb.2018.00384.

Short communication: Lymphocyte proliferative responses in cattle naturally infected with digital dermatitis consists of CD8+ and gamma delta T cells but lack CD4+ T cells - (Peer Reviewed Journal)
Wilson-Welder, J.H., Nally, J.E., Alt, D.P., Humphrey, S.B., Olsen, S.C. 2018. Short communication: Lymphocyte proliferative responses in cattle naturally infected with digital dermatitis consists of CD8plus and gamma delta T cells but lack CD4plus T cells. Journal of Dairy Science. 101(9):8301-8307. https://doi.org/10.3168/jds.2017-13913.

Isolation and characterization of pathogenic leptospires associated with cattle - (Peer Reviewed Journal)
Nally, J.E., Hornsby, R.L., Alt, D.P., Bayles, D.O., Wilson-Welder, J.H., Bauer, N.E. 2018. Isolation and characterization of pathogenic leptospires associated with cattle. Veterinary Microbiology. 218:25-30. https://doi.org/10.1016/j.vetmic.2018.03.023.

Inbred rats as a model to study persistent renal colonization and associated cellular immune responsiveness - (Peer Reviewed Journal)
Nally, J.E., Wilson-Welder, J.H., Hornsby, R.L., Palmer, M.V., Alt, D.P. 2018. Inbred rats as a model to study persistent renal colonization and associated cellular immune responsiveness. Frontiers in Cellular and Infection Microbiology. https://doi.org/10.3389/fcimb.2018.00066.

Pathogenic leptospires modulate protein expression and post-translational modifications in response to mammalian host signals - (Peer Reviewed Journal)
Nally, J.E., Grassmann, A.A., Planchon, S., Sergeant, K., Renaut, J., Sesku, J., McBride, A., Caimano, M.J. 2017. Pathogenic leptospires modulate protein expression and post-translational modifications in response to mammalian host signals. Frontiers in Cellular and Infection Microbiology. 7:362. https://doi.org/10.3389/fcimb.2017.00362.

Treponeme-associated hoof disease of wild elk (Cervus elaphus spp.) in southwestern Washington State, USA - (Peer Reviewed Journal)
Han, S., Mansfield, K., Haldorson, G., Bradway, D., Besser, T., Reed, D., Alt, D.P., Wilson-Welder, J.H. 2018. Treponeme-associated hoof disease of wild elk (Cervus elaphus spp.) in southwestern Washington State, USA. Veterinary Pathology. https://doi.org/10.1177/0300985818798108.

Dr. James N. Miller; Virtuoso of All Spirochetes - (Other)
Nally, J.E. 2016. Dr. James N. Miller; Virtuoso of All Spirochetes. Forum on Immunopathological Diseases and Therapeutics. Volume 7/Issue 3-4. doi: 10.1615/forumimmundisther.2017020504.