Research Project: Characterization and Management of Citrus Pathogens Transmitted by Phloem-Feeding Insect Vectors

Location: Crop Diseases, Pests and Genetics Research

Publications (Clicking on the reprint icon will take you to the publication reprint.)

Current research to control agricultural pests and disease vectors at the USDA-ARS San Joaquin Valley Agricultural Sciences Center - (Abstract Only)

An improved Recombinase polymerase amplification coupled with lateral flow assay for rapid field detection of "Candidatus Liberibacter asiaticus" - (Peer Reviewed Journal)
Rattner, R., Godfrey, K., Hajeri, S., Yokomi, R.K. 2022. An improved Recombinase polymerase amplification coupled with lateral flow assay for rapid field detection of "Candidatus Liberibacter asiaticus". Plant Disease. https://doi.org/10.1094/PDIS-09-21-2098-RE.

Citrus tristeza virus - (Book / Chapter)
Hajeri, S., Yokomi, R.K. 2023. Citrus tristeza virus. In: Gaur, R.K., Patil, B.L., Selvarajan, R., editors. Plant RNA Viruses. 1st edition. Cambridge, MA: Academic Press. p.117-133. https://doi.org/10.1016/B978-0-323-95339-9.00009-0.

Estimated incidence and genotypes of CTV in Southern California - (Abstract Only)
Yokomi, R.K., Rattner, R., Hajeri, S. 2022. Estimated incidence and genotypes of CTV in Southern California. California Citrus Conference. Citrus Research Board.

Recombinase polymerase amplification coupled with lateral flow assay for rapid field detection of “Candidatus Liberibacter asiaticus” in citrus plants - (Abstract Only)
Rattner, R., Hajeri, S., Godfrey, K., Yokomi, R.K. 2021. Recombinase polymerase amplification coupled with lateral flow assay for rapid field detection of “Candidatus Liberibacter asiaticus” in citrus plants. California Citrus Conference. Citrus Research Board.

Evaluation of reliable sampling tissue and seasonality for consistent detection of CLas by qPCR - (Abstract Only)
Hajeri, S., Kumagai, L., Yokomi, R.K. 2021. Evaluation of reliable sampling tissue and seasonality for consistent detection of CLas by qPCR. California Citrus Conference. Citrus Research Board.

Host metabolomic responses to CTV infection - (Abstract Only)
Wallis, C.M., Rattner, R., Gorman, Z., Hajeri, S., Yokomi, R.K. 2021. Host metabolomic responses to CTV infection. Meeting Abstract. Proceedings of the 2021 Citrus Research Conference.

Genome analysis of Spiroplasma citri strains from different host plants and its leafhopper vectors - (Peer Reviewed Journal)
Rattner, R., Thapa, S.P., Dang, T., Osman, F., Selvaraj, V., Maheshwari, Y., Pagliaccia, D., Espindola, A.S., Hajeri, S., Chen, J., Coaker, G., Vidalakis, G., Yokomi, R.K. 2021. Genome analysis of Spiroplasma citri strains from different host plants and its leafhopper vectors. BMC Genomics. 22:373. https://doi.org/10.1186/s12864-021-07637-8.

Impact of MCA13-Reactive CTV and its eradication in commercial citrus - (Other)
Yokomi, R.K., Rattner, R., Hajeri, S. 2021. Impact of MCA13-Reactive CTV and its eradication in commercial citrus. Citrograph.

Multiplex detection of candidatus liberibacter asiaticus and Spiroplasma citri by qPCR and droplet digital PCR - (Peer Reviewed Journal)
Maheshwari, Y., Sevaraj, V., Godfrey, K., Hajeri, S., Yokomi, R.K. 2021. Multiplex detection of “Candidatus Liberibacter asiaticus” and Spiroplasma citri by qPCR and droplet digital PCR. PLoS ONE. 16(3):e0242392. https://doi.org/10.1371/journal.pone.0242392.

Improvements to HLB and stubborn testing - (Trade Journal)
Yokomi, R.K., Selvaraj, V., Maheshwari, Y., Rattner, R., Godfrey, K.E., Chen, J., Hajeri, S. 2021. Improvements to HLB and stubborn testing. Citrograph. 12(2):64-68.

Detection of viroids using RT-qPCR - (Book / Chapter)
Hajeri, S., Vidalakis, G., Yokomi, R.K. 2021. Detection of viroids using RT-qPCR. In: Rao, A.L.N., Lavagi-Craddock, I., Vidalakis, G., editors. Viroids. Methods in Molecular Biology, vol 2316. New York, NY: Humana. p. 153-162. https://doi.org/10.1007/978-1-0716-1464-8_14.

Immunocapture-reverse transcriptase loop-mediated isothermal amplification assay for detection of plant RNA viruses - (Book / Chapter)
Hajeri S., Yokomi R. 2021. Immunocapture-reverse transcriptase loop-mediated isothermal amplification assay for detection of plant RNA viruses. In: Wang, A., Li, Y., editors. Plant Virology. Methods in Molecular Biology, vol 2400. New York, NY: Humana. p. 245-252. https://doi.org/10.1007/978-1-0716-1835-6_23.

Whole genome sequence of five strains of Spiroplasma citri isolated from different host plants and its leafhopper vector - (Peer Reviewed Journal)
Yokomi, R.K., Rattner, R., Osman, F., Maheshwari, Y., Selvaraj, V., Pagliaccia, D., Chen, J., Vidalakis, G. 2020. Whole genome sequence of five strains of Spiroplasma citri isolated from different host plants and its leafhopper vector. BMC Research Notes. 13. Article 320. https://doi.org/10.1186/s13104-020-05160-9.

Genetically-engineered citrus tristeza virus: establishing and examining its interaction with wild type CTV - (Trade Journal)
Yokomi, R.K., Selvaraj, V., Maheshwari, Y., Hajeri, S. 2020. Genetically-engineered citrus tristeza virus: establishing and examining its interaction with wild type CTV. Citrograph. 10(4):66-69.

Spread of citrus tristeza virus in central California - (Abstract Only)
Yokomi, R.K., Sisterson, M.S., Hajeri, S. 2021. Spread of citrus tristeza virus in central California. Phytopathology. 110:S2.1. https://doi.org/10.1094/PHYTO-110-12-S2.1.

Spread of Citrus tristeza virus in citrus orchards in central California - (Peer Reviewed Journal)
Yokomi, R.K., Sisterson, M.S., Hajeri, S. 2020. Spread of Citrus tristeza virus in citrus orchards in central California. Plant Disease. 104(7):1925-1931. https://doi.org/10.1094/PDIS-08-19-1791-RE.

Reliable sampling tissue and seasonality for consistent detection of 'Candidatus Liberibacter asiaticus' by qPCR - (Peer Reviewed Journal)
Hajeri, S., Yokomi, R.K. 2020. Reliable sampling tissue and seasonality for consistent detection of 'Candidatus Liberibacter asiaticus' by qPCR. Current Agriculture Research Journal. 8(1). https://doi.org/10.12944/CARJ.8.1.01.

Genome sequence resource for Spiroplasma citri, strain CC-2, associated with citrus stubborn disease in California - (Peer Reviewed Journal)
Yokomi, R.K., Chen, J., Rattner, R., Selvaraj, V., Majeshwari, Y., Osman, F., Pagliacica, D., Vidalakis, G. 2019. Genome sequence resource for Spiroplasma citri, strain CC-2, associated with citrus stubborn disease in California. Phytopathology. 110(2):254-256. https://doi.org/10.1094/PHYTO-08-19-0304-A.

A rapid detection tool for VT isolates of Citrus tristeza virus by immunocapture- reverse transcriptase loop mediated isothermal amplification assay - (Peer Reviewed Journal)
Selvaraj, V., Maheshwari, Y., Hajeri, S., Yokomi, R.K. 2019. A rapid detection tool for VT isolates of Citrus tristeza virus by immunocapture- reverse transcriptase loop mediated isothermal amplification assay. PLoS One. 14(9):e0222170. https://doi.org/10.1371/journal.pone.0222170.

Droplet digital PCR for absolute quantification of plant pathogens - (Book / Chapter)
Selvaraj V., Maheshwari Y., Hajeri S., Yokomi R. 2019. Droplet digital PCR for absolute quantification of plant pathogens. In: Khurana S., Gaur R., editors. Plant Biotechnology: Progress in Genomic Era. Singapore: Springer. p. 583-595.

Development and validation of a Loop-Mediated Isothermal Amplification technique (LAMP) for the detection of Spiroplasma citri, the causal agent of citrus stubborn disease - (Peer Reviewed Journal)
Drais, M.D., Maheshwari, Y., Selvaraj, V., Varvaro, L., Yokomi, R.K., Djelouah, K. 2019. Development and validation of a Loop-Mediated Isothermal Amplification technique (LAMP) for the detection of Spiroplasma citri, the causal agent of citrus stubborn disease. European Journal of Plant Pathology. 155(1):125-134. https://doi.org/10.1007/s10658-019-01755-6.

High-risk based field survey and high-throughput qPCR detection system for Huanglongbing-associated bacteria in commercial groves in California - (Abstract Only)
Hajeri, S., Liao, W., Westerman, K., Barnier, J., Luo, W., Gottwald, T.R., Yokomi, R.K. 2019. High-risk based field survey and high-throughput qPCR detection system for Huanglongbing-associated bacteria in commercial groves in California. International Research Conference on Huanglongbing. p. 5-61.

Progress on the development of a field-use optical sensor for screening of citrus pathogens in California - (Abstract Only)
Edwards, P., Ochochi, B., Liu, Z., Hajeri, S., Yokomi, R.K. 2019. Progress on the development of a field-use optical sensor for screening of citrus pathogens in California. International Research Conference on Huanglongbing. p. 4-57.

Progress on the development of a field-use optical sensor for screening of citrus pathogens in California - (Abstract Only)
Drais, M., Maheshwari, Y., Selvaraj, Y., Varvaro, L., Yokomi, R.K., Djelouah, K. 2019. Progress on the development of a field-use optical sensor for screening of citrus pathogens in California. Conference of International Organization of Citrus Virologists. p. 7-24.

“Candidatus Liberibacter asiaticus” strains from multiple locations in southern California are different - (Abstract Only)
Dai, Z., Wu, F., Zheng, Z., Yokomi, R.K., Kumagai, L., Cai, W., Rascoe, J., Polek, M., Deng, Z. 2019. “Candidatus Liberibacter asiaticus” strains from multiple locations in southern California are different. International Research Conference on Huanglongbing, March 10-15, 2019, Riverside, California.

Characterization of California Citrus tristeza virus (CTV) strains that react with CTV MCA13 monoclonal antibody - (Abstract Only)

Identification and characterization of RB isolates of citrus tristeza virus - (Book / Chapter)
Saponari, M., Giampetruzzi, A., Selvaraj, V., Maheshwari, Y., Yokomi, R.K. 2019. Identification and characterization of RB isolates of citrus tristeza virus. In: Catara A., Bar-Joseph M., Licciardello G., editors. Citrus Tristeza Virus. Methods in Molecular Biology. New York, NY: Humana Press. p. 105-126.

CTV vectors and interactions with the virus and host plants - (Book / Chapter)
Yokomi, R.K. 2019. CTV vectors and interactions with the virus and host plants. In: Catara, A., Bar-Joseph, M., Licciardello, G., editors. Citrus Tristeza Virus. Methods in Molecular Biology. New York, NY: Humana Press. p. 29-53.

Prophage diversity of “Candidatus Liberibacter asiaticus” strains in California - (Peer Reviewed Journal)
Dai, Z., Wu, F., Zheng, Z., Yokomi, R.K., Kumagai, L., Cai, W., Rasco, J., Polek, M., Deng, Z., Chen, J. 2019. Prophage diversity of “Candidatus Liberibacter asiaticus” strains in California. Phytopathology. 109(4):551-559. https://doi.org/10.1094/PHYTO-06-18-0185-R.

Nucleotide heterogeneity at the terminal ends of the genomes of two California Citrus tristeza virus strains and their complete genome sequence analysis - (Peer Reviewed Journal)
Chen, A.Y., Watanabe, S., Yokomi, R.K., Ng, J.C. 2018. Nucleotide heterogeneity at the terminal ends of the genomes of two California Citrus tristeza virus strains and their complete genome sequence analysis. Virology Journal. 15(1):141. https://doi.org/10.1186/s12985-018-1041-4.

Development of a duplex droplet digital PCR assay for absolute quantitative detection of "Candidatus Liberibacter asiaticus" - (Peer Reviewed Journal)
Selvaraj, V., Maheshwari, Y., Hajeri, S., Chen, J., Mccollum, T.G., Yokomi, R.K. 2018. Development of a duplex droplet digital PCR assay for absolute quantitative detection of "Candidatus Liberibacter asiaticus". PLoS One. 13(5):e0197184. https://doi.org/10.1371/journal.pone.0197184.

Description of a novel mild strain of Citrus tristeza virus in California that reacts with monoclonal antibody MCA13 - (Abstract Only)

Preinfection of citrus by RB strains of Citrus tristeza virus (CTV) negatively affected expression of a modified T36 CTV vector - (Abstract Only)

Diversity of “Candidatus Liberibacter asiaticus” strains in California - (Abstract Only)
Dai, Z., Wu, F., Zheng, Z., Yokomi, R.K., Kumagai, L., Cai, W., Rascoe, J., Polek, M., Deng, X., Chen, J. 2018. Diversity of “Candidatus Liberibacter asiaticus” strains in California. Phytopathology. 108:S1.93. https://doi.org/10.1094/PHYTO-108-10-S1.1.

Application of duplex droplet digital PCR for detection of “Candidatus Liberibacter asiaticus” using 16S rRNA and ribonucleotide reductase genes - (Abstract Only)

California mild CTV strains that break resistance in Trifoliate Orange - (Trade Journal)
Yokomi, R.K., Selvaraj, V., Maheshwari, Y., Hajeri, S. 2018. California mild CTV strains that break resistance in Trifoliate Orange. Citrograph. 9(2):66-69.