Research Project: Genetic Improvement of Citrus for Enhanced Resistance to Huanglongbing Disease and Other Stresses

Location: Subtropical Insects and Horticulture Research

Publications (Clicking on the reprint icon will take you to the publication reprint.)

Response of citrus germplasm seedlings to Candidatus Liberibacter Asiaticus infection under controlled greenhouse conditions - (Peer Reviewed Journal)
Bisi, R., Albrecht, U., Bowman, K.D. 2024. Response of citrus germplasm seedlings to Candidatus Liberibacter Asiaticus infection under controlled greenhouse conditions. HortScience. 59(3), 278-287. https://doi.org/10.21273/HORTSCI17514-23.

Improving citrus bud grafting efficiency. - (Peer Reviewed Journal)
Niedz, R.P., Bowman, K.D. 2023. Improving citrus bud grafting efficiency. Scientific Reports. 13, 17807. https://doi.org/10.1038/s41598-023-44832-x.

Four new SuperSour rootstocks for improved production of sweet orange in a Huanglongbing environment - (Peer Reviewed Journal)
Bowman, K.D. 2023. Four new SuperSour rootstocks for improved production of sweet orange in a Huanglongbing environment. HortScience. 58(12), 1622-1625. https://doi.org/10.21273/HORTSCI17466-23.

Endophytes and plant extracts as potential antimicrobial agents against Candidatus Liberibacter asiaticus, causal agent of huanglongbing - (Peer Reviewed Journal)
Krystel, J.A., Stover, E.W., Dominguez, J., Jayachandran, K., Shetty, K. 2023. Endophytes and plant extracts as potential antimicrobial agents against Candidatus Liberibacter asiaticus, causal agent of huanglongbing. Microorganisms. https://doi.org/10.3390/microorganisms11061529.

Delivery of CRISPR-Cas12a ribonucleoprotein complex for genome editing in an embryogenic citrus cell line - (Peer Reviewed Journal)
Fong, H., Culver, J.N., Niedz, R.P., Qi, Y. 2023. Delivery of CRISPR-Cas12a ribonucleoprotein complex for genome editing in an embryogenic citrus cell line. Methods in Molecular Biology. https://doi.org/10.1007/978-1-0716-3131-7_10.

Relative influence of rootstock and scion on Asian citrus psyllid colonization and Candidatus Liberibacter asiaticus distribution - (Peer Reviewed Journal)
Tardivo, C., Qureshi, J., Bowman, K.D., Albrecht, U. 2023. Relative influence of rootstock and scion on Asian citrus psyllid colonization and Candidatus Liberibacter asiaticus distribution. Scientia Horticulturae. 58(4):395-403. https://doi.org/10.21273/HORTSCI17039-22.

Relative influence of rootstock and scion on Asian Citrus psyllid infestation and Candidatus Liberibacter asiaticus colonization - (Peer Reviewed Journal)
Tardivo, C., Qureshi, J., Bowman, K.D., Albrecht, U. Relative influence of rootstock and scion on Asian Citrus psyllid infestation and Candidatus Liberibacter asiaticus colonization. HortScience. 58(4):395–403. 2023. https://doi.org/10.21273/HORTSCI17039-22.

Genetic modulation of valencia sweet orange field performance by 50 rootstocks under huanglongbing-endemic conditions - (Peer Reviewed Journal)
Bowman, K.D., Mccollum, G.T., Seymore, D. 2023. Genetic modulation of valencia sweet orange field performance by 50 rootstocks under huanglongbing-endemic conditions. Frontiers in Plant Science. https://doi.org/10.3389/fpls.2023.1061663.

Register of new fruit and nut cultivars list 51 - (Peer Reviewed Journal)
Bowman, K.D., Grosser, J., Karp, D. Register of new fruit and nut cultivars list 51. HortScience. 57(9):1174-1233. 2022. https://doi.org/10.21273/HORTSCI.57.9.1174.

Highly efficient genome editing in plant protoplasts by Ribonucleoprotein delivery of CRISPR-Cas12a nucleases - (Peer Reviewed Journal)
Zhang, Y., Cheng, Y., Fang, H., Roberts, N., Zhang, L., Vakulskas, C.A., Niedz, R.P., Culver, J.N., Qi, Y. 2022. Highly efficient genome editing in plant protoplasts by Ribonucleoprotein delivery of CRISPR-Cas12a nucleases. Frontiers in Genome Editing. 4: Article 780238. https://doi.org/10.3389/fgeed.2022.780238.

Genome-Wide association study of healthful flavonoids among diverse mandarin accessions - (Peer Reviewed Journal)
Mattia, M.R., Du, D., Yu, Q., Kahn, T.L., Roose, M.L., Hiraoka, Y., Wang, Y., Munoz, P.T., Gimitter, F.G. 2022. Genome-Wide association study of healthful flavonoids among diverse mandarin accessions. Plants. 11(3):317. https://doi.org/10.3390/plants11030317.

Planting in metallized reflective mulch did not significantly benefit development of new hybrid seedlings - (Peer Reviewed Journal)
Stover, E.W., Mayo, S., Driggers, R.E., Adair, R.C. 2022. Planting in metallized reflective mulch did not significantly benefit development of new hybrid seedlings. HortScience. 57:2. https://doi.org/10.21273/HORTSCI16248-21.

Graft compatibility of new scion-rootstock combinations - (Trade Journal)
Albrecht, U., Meyering, B., Chaparro, J., Bowman, K.D. 2021. Graft compatibility of new scion-rootstock combinations. Citrus Industry. pp 10-13. https://crec.ifas.ufl.edu/media/crecifasufledu/extension/extension-publications/2021/2021_november_graft.pdf

SuperSour: A new strategy for breeding superior citrus rootstocks - (Peer Reviewed Journal)
Bowman, K.D., Mccollum, T.G., Albrecht, U. 2021. SuperSour: A new strategy for breeding superior citrus rootstocks. Frontiers in Plant Science. 12, 741009. https://doi.org/10.3389/fpls.2021.741009.

Different sweet orange-rootstock combinations infected by Candidatus Liberibacter asiaticus under greenhouse conditions: Effects on the roots - (Peer Reviewed Journal)
Bodaghi, S., Pugina, G., Meyering, B., Bowman, K.D., Albrecht, U. 2022. Different sweet orange-rootstock combinations infected by Candidatus Liberibacter asiaticus under greenhouse conditions: Effects on the roots. HortScience. 57(1), 56-64. https://doi.org/10.21273/HORTSCI16206-21.

Different sweet orange-rootstock combinations infected by Candidatus Liberibacter asiaticus under greenhouse conditions: Effects on the scion - (Peer Reviewed Journal)
Bodaghi, S., Meyering, B., Bowman, K.D., Albrecht, U. 2021. Different sweet orange-rootstock combinations infected by Candidatus Liberibacter asiaticus under greenhouse conditions: Effects on the scion. HortScience. 57(1), 144-153. https://doi.org/10.21273/HORTSCI16205-21.

Incidence of Asiatic citrus canker on Trifoliate orange and its hybrid accessions in a Florida field planting - (Peer Reviewed Journal)
Stover, E.W., Ramadugu, C., Roose, M., Krystel, J.A., Lee, R.F., Keremane, M.L. 2021. Incidence of Asiatic citrus canker on Trifoliate orange and its hybrid accessions in a Florida field planting. Journal of the American Society for Horticultural Science. 56(5):525–531. https://doi.org/10.21273/HORTSCI15684-20.

Horticultural attributes and root architectures of field-grown ‘Valencia’ trees grafted on different rootstocks propagated by seed, cuttings, and tissue culture - (Peer Reviewed Journal)
Pokhrel, S., Meyering, B., Bowman, K.D., Albrecht, U. 2020. Horticultural attributes and root architectures of field-grown ‘Valencia’ trees grafted on different rootstocks propagated by seed, cuttings, and tissue culture. HortScience. 56(2):163–172. https://doi.org/10.21273/HORTSCI15507-20.

Rootstock influences on health and growth following Candidatus Liberibacter asiaticus infection in young sweet orange trees - (Peer Reviewed Journal)
Bowman, K.D., Albrecht, U. 2020. Rootstock influences on health and growth following Candidatus Liberibacter asiaticus infection in young sweet orange trees. Agronomy. 10(12):1907. https://doi.org/10.3390/agronomy10121907.

Identification of citrus immune regulators involved in defence against Huanglongbing using a new functional screening system - (Peer Reviewed Journal)
Huang, C., Niu, D., Kund, G., Jones, M., Albrecht, U., Nguyen, L., Bui, C., Ramadugu, C., Bowman, K.D., Trumble, J., Jin, H. 2020. Identification of citrus immune regulators involved in defence against Huanglongbing using a new functional screening system. Plant Biotechnology Journal. 19(4):757-766. https://doi.org/10.1111/pbi.13502.

Improving Winter Growth in the Citrus Nursery with LED and HPS Supplemental Lighting - (Peer Reviewed Journal)
Bowman, K.D., Albrecht, U. 2020. Improving Winter Growth in the Citrus Nursery with LED and HPS Supplemental Lighting. HortScience. 56(1):21-27. https://doi.org/10.21273/HORTSCI15302-20.

Flowering locus T chimeric protein induces floral precocity in edible citrus - (Peer Reviewed Journal)
Sinn, J., Held, J., Vosburg, C., Klee, S., Orbovic, V., Taylor, E.L., Gottwald, T.R., Stover, E.W., Moore, G., McNellis, T. 2020. Flowering locus T chimeric protein induces floral precocity in edible citrus. Plant Biotechnology Journal. https://doi.org/10.1111/pbi.13463.

Fractional factorial designs: An underutilized tool for rapid in vitro system development - (Peer Reviewed Journal)
Niedz, R.P. 2021. Fractional factorial designs: An underutilized tool for rapid in vitro system development. Methods in Molecular Biology. 2289:23-45. https://doi.org/10.1007/978-1-0716-1331-3_2.

Huanglongbing disease putting the squeeze on OJ production in Florida - (Abstract Only)
Stover, E.W. 2020. Huanglongbing disease putting the squeeze on OJ production in Florida. American Society of Horticulture Science Meeting. P.34072. https://ashs.confex.com/ashs/2020/meetingapp.cgi/Paper/34072.

Rationale for reconsidering current regulations restricting use of hybrids in orange juice - (Peer Reviewed Journal)
Stover, E., Gmitter, F.G., Grosser, J., Baldwin, E., Wu, G.A., Bai, J., Wang, Y., Chaires, P., Motamayor, J.C. 2020. Rationale for reconsidering current regulations restricting use of hybrids in orange juice. Horticulture Research. 7:38. https://doi.org/10.1038/s41438-020-0277-5.

Citrus Rootstocks - (Book / Chapter)
Bowman, K.D., Joubert, J. 2020. Citrus Rootstocks. Book Chapter. 105-127. 2020. https://doi.org/10.1016/B978-0-12-812163-4.00006-1.

Seed and seedling nursery characteristics for 10 USDA citrus rootstocks - (Peer Reviewed Journal)
Bisi, R., Albrecht, U., Bowman, K.D. 2020. Seed and seedling nursery characteristics for 10 USDA citrus rootstocks. HortScience. 55(4):528-532. https://doi.org/10.21273/HORTSCI14598-19.

Evaluating a DOE screen to reduce hyperhydricity in the threatened plant, Cycladenia humilis var. jonesii - (Peer Reviewed Journal)
Pence, V.C., Finke, L.R., Niedz, R.P. 2020. Evaluating a DOE screen to reduce hyperhydricity in the threatened plant, Cycladenia humilis var. jonesii. In Vitro Cellular and Developmental Biology - Plants. 56:215–229. https://doi.org/10.1007/s11627-019-10038-y.

Rootstock influences the metabolic response to Candidatus Liberibacter asiaticus in grafted sweet orange trees - (Peer Reviewed Journal)
Albrecht, U., Tripathi, I., Bowman, K.D. 2019. Rootstock influences the metabolic response to Candidatus Liberibacter asiaticus in grafted sweet orange trees. Trees. 34:405-431. https://doi.org/10.1007/s00468-019-01925-3.

Florida citrus rootstock selection guide online tool - (Other)
Castle, W.S., Bowman, K.D., Grosser, J.W., Futch, S.H., Graham, J.H. 2019. Florida citrus rootstock selection guide online tool. Univerity of Florida EDIS. HS1260.

An in vitro protocol for rapidly assessing the effects of antimicrobial compounds on the unculturable bacterial plant pathogen, Candidatus Liberibacter asiaticus - (Peer Reviewed Journal)
Krystel, J.A., Shi, Q., Shaw, J.J., Goutam, G., Hall, D.G., Stover, E.W. 2019. An in vitro protocol for rapidly assessing the effects of antimicrobial compounds on the unculturable bacterial plant pathogen, Candidatus Liberibacter asiaticus. Plant Methods. 15:85. https://doi.org/10.1186/s13007-019-0465-1

Reciprocal influences of rootstock and scion citrus cultivars challenged with Ca. Liberibacter asiaticus - (Peer Reviewed Journal)
Albrecht, U., Bowman, K. 2019. Reciprocal influences of rootstock and scion citrus cultivars challenged with Ca. Liberibacter asiaticus. Scientia Horticulturae. 254:133-142. https://doi.org/10.1016/j.scienta.2019.05.010

Hexaacetyl-chitohexaose, a chitin-derived oligosaccharides, transiently activiates citrus defenses and alters the feeding behavior of asian citrus psyllid - (Peer Reviewed Journal)
Shi, Q., George, J., Krystel, J.A., Zhang, S., Lapointe, S.L., Stelinski, L.L., Stover, E.W. 2019. Hexaacetyl-chitohexaose, a chitin-derived oligosaccharides, transiently activiates citrus defenses and alters the feeding behavior of asian citrus psyllid. Horticulture Research. 6(76):1-10. https://doi.org/10.1038/s41438-019-0158-y.

Temporal and spatial detection of Candidatus Liberibacter asiaticus putative effector transcripts during interaction with Huanglongbing-susceptible, -tolerant, and -resistant citrus hosts - (Peer Reviewed Journal)
Shi, Q., Pitino, M., Zhang, S., Krystel, J.A., Cano, L.M., Shatters, R.G., Hall, D.G., Stover, E.W. 2019. Temporal and spatial detection of Candidatus Liberibacter asiaticus putative effector transcripts during interaction with Huanglongbing-susceptible, -tolerant, and -resistant citrus hosts. Biomed Central (BMC) Plant Biology. 19:122. https://doi.org/10.1186/s12870-019-1703-4.

USDA efforts to develop resistance and tolerance to huanglongbing in citrus scions - (Abstract Only)
Stover, E.W., Driggers, R.E., Krystel, J.A., Hao, G., Hall, D.G., Gupta, G., Hartung, J.S., Baldwin, E.A., Bai, J., Plotto, A., Manthey, J.A. 2019. USDA efforts to develop resistance and tolerance to huanglongbing in citrus scions[abstract]. International Research Conference on Huanglongbing, March 12-15, 2019, Riverside, California.

Minnie finger lime: A new novelty citrus cultivar - (Peer Reviewed Journal)
Bowman, K.D., McCollum, T.G., Plotto, A., Bai, J. 2019. Minnie finger lime: A new novelty citrus cultivar. HortScience. 54(8):1425-1428. https://doi.org/10.21273/HORTSCI13622-18.

Walter Tennyson Swingle, a relentless intellect that transformed American pomology - (Peer Reviewed Journal)
Stover, E.W., Wright, G. 2019. Walter Tennyson Swingle, a relentless intellect that transformed American pomology. Journal of American Pomological Society. 73:129-138.

Huanglongbing-related responses of 'Valencia' sweet orange on eight citrus rootstocks during greenhouse trials - (Peer Reviewed Journal)
Stover, E.W., Hall, D.G., Grosser, J., Gruber, B., Moore, G. 2018. Huanglongbing-related responses of 'Valencia' sweet orange on eight citrus rootstocks during greenhouse trials. HortTechnology. 28(6):776-782. https://doi.org/10.21273/HORTTECH04137-18.

Efficiency of biodegradable and pH-responsive polysuccinimide nanoparticles (PSI-NPs) as smart nanodelivery systems in grapefruit: in vitro cellular investigation - (Peer Reviewed Journal)
Xin, X., He, Z., Hill, M.R., Niedz, R.P., Jiiang, X., Sumerlin, B.S. 2018. Efficiency of biodegradable and pH-responsive polysuccinimide nanoparticles (PSI-NPs) as smart nanodelivery systems in grapefruit: in vitro cellular investigation. In Vitro Cellular and Developmental Biology. 18(7):e1800159. https://doi.org/10.1002/mabi.201800159.