Location: Corn Insects and Crop Genetics Research
Project Number: 5030-21220-068-003-I
Project Type: Interagency Reimbursable Agreement
Start Date: Jun 1, 2020
End Date: May 31, 2025
Objective:
Determine regulators of immunity to powdery mildew in barley and validate role of cis-regulatory element (CRM) - regulated genes
Approach:
SPECIFIC AIM 1: Use genome-wide promoter-motif enrichment strategies to uncover master regulators of immunity
With the advent of a new high-quality, megabase resolution barley assembly, our aim is to leverage our accumulated expression data to facilitate identification of cis-regulatory elements (CRM), and associated expression cassettes associated with immunity.
SPECIFIC AIM 2: Functional validation of cis-regulatory element (CRM)-regulated genes and associated targets via reverse genetic analysis and high-throughput phenotyping