Location: Vegetable Crops Research
Project Number: 5090-21000-073-056-A
Project Type: Cooperative Agreement
Start Date: Sep 1, 2020
End Date: Jul 31, 2024
Objective:
1. Develop and characterize cucumber core population (390 cucumber lines).
2. Molecular mapping of downy mildew (DM) resistances in cucumber inbred line PT108.
3. Marker-assisted pyramiding of DM resistance QTL and evaluation of enhanced germplasm with multiple disease resistances.
Approach:
Objective 1:
1) Re-sequencing and analyze the data for 384 cucumber lines.
2) Phenotype for target traits in this collection for downy mildew resistance.
3) Conduct association analysis for downy mildew resistance.
Objective 2:
1) Fine mapping dm4.1, dm5.2, and dm5.3 major-effect QTL.
2) QTL mapping of DM resistance in PT108 from exotic source.
Objective 3:
3). Marker-assisted QTL pyramiding of dm4.1, dm5.2 and dm5.3 into elite pickling cucumber background Gy14. Evaluate its performance in field and growth chambers.
