Location: Crop Production and Protection
Project Number: 0500-00053-005-137-G
Project Type: Grant
Start Date: May 1, 2022
End Date: Apr 30, 2025
Objective:
1. Generate transgenic barley expressing both CRISPR/Cas9 and Fhb7 donor.
2. Evaluate the Fhb7 function in transgenic barley.
3. Screen the transgenic plants for targeted Fhb7 insertion events.
Approach:
1. Develop 90 transgenic plants by Agrobacterium-mediation and Biolistic bombardment.
2. Characterize Fhb7 function in barley by examining the transgenic plants for Fusarium Head Blight (FHB) resistance and DON accumulation.
3. Screen large populations of transgenic plants for the Fhb7 insertion events by PCR assays and deliver Fhb7-insertion lines as novel germplasm.
