Research Project: Control Strategies for Bovine Babesiosis

Location: Animal Disease Research

Publications (Clicking on the reprint icon will take you to the publication reprint.)

Haemaphysalis longicornis (Acari: Ixodidae) does not transmit Babesia bovis, a causative agent of cattle fever - (Peer Reviewed Journal)
Poh, K.C., Aguilar, M., Capelli-Peixoto, J., Davis, S.K., Ueti, M.W. 2024. Haemaphysalis longicornis (Acari: Ixodidae) does not transmit Babesia bovis, a causative agent of cattle fever. Ticks and Tick Borne Diseases. 15:(6). https://doi.org/10.1016/j.ttbdis.2024.102374.

Development and application of an in vitro tick feeding system to identify ixodes tick environment-induced genes of the lyme disease agent, Borrelia burgdorferi - (Peer Reviewed Journal)
Yamasaki, Y., Singh, P., Vimonish, R., Ueti, M.W., Bankhead, T. 2024. Development and application of an in vitro tick feeding system to identify ixodes tick environment-induced genes of the lyme disease agent, Borrelia burgdorferi. Pathogens. 13:(487). https://doi.org/10.3390/pathogens13060487.

Knowledge and perception of equine ticks and tick-borne diseases of Pennsylvania horse owners and caretakers - (Peer Reviewed Journal)
Smarsh, D.N., Kenny, L.B., Spindler, M., Poh, K.C., Machtinger, E.T. 2024. Knowledge and perception of equine ticks and tick-borne diseases of Pennsylvania horse owners and caretakers. Journal of Equine Veterinary Science. https://doi.org/10.1016/j.jevs.2024.105092.

Comparative efficacy of buparvaquone and imidocarb in inhibiting the in vitro growth of Babesia bovis - (Peer Reviewed Journal)
Cardillo, N.M., Lacy, P.A., Villarino, N.F., Dogget, S.J., Riscoe, M.K., Bastos, R.G., Laughery, J.M., Ueti, M.W., Suarez, C.E. 2024. Comparative efficacy of buparvaquone and imidocarb in inhibiting the in vitro growth of Babesia bovis. FRONTIERS IN PHARMACOLOGY. https://doi.org/10.3389/fphar.2024.1407548.

A conserved motif in the immune-subdominant RAP-1 Related Antigen (RRA) of Babesia bovis contains a B-cell epitope recognized by antibodies from protected cattle - (Peer Reviewed Journal)
Rojas, M.J., Bastos, R.G., Navas, J., Laughery, J.M., Lacy, P.A., Suarez, C.E. 2024. A conserved motif in the immune-subdominant RAP-1 Related Antigen (RRA) of Babesia bovis contains a B-cell epitope recognized by antibodies from protected cattle. Frontiers in Immunology. 15 - 2024. https://doi.org/10.3389/fimmu.2024.1380660.

Biochemical analysis of Hyalomma dromedarii salivary glands and gut tissues using SR-FTIR micro-spectroscopy - (Peer Reviewed Journal)
Hendaway, S.H., Alzan, H.F., Abdel-Ghany, H.S., Suarez, C.E., Kamel, G. 2024. Biochemical analysis of Hyalomma dromedarii salivary glands and gut tissues using SR-FTIR micro-spectroscopy. Scientific Reports. (14):8515. https://doi.org/10.1038/s41598-024-59165-6.

Expression of sex-specific molecular markers by Babesia bovis gametes - (Peer Reviewed Journal)
Hussein, H.E., Johnson, W.C., Taus, N.S., Ueti, M.W. 2024. Expression of sex-specific molecular markers by Babesia bovis gametes. Parasites & Vectors. 17:(75). https://doi.org/10.1186/s13071-024-06185-w.

Immunization of cattle with a Rhipicephalus microplus chitinase peptide containing predicted B-cell epitopes reduces tick biological fitness - (Peer Reviewed Journal)
Perez-Soria, M.E., López-Díaz, G., Jiménez-Ocampo, R., Aguilar-Tipacamu, G., Ueti, M.W., Mosqueda, J. 2024. Immunization of cattle with a Rhipicephalus microplus chitinase peptide containing predicted B-cell epitopes reduces tick biological fitness. Cambridge University Press. https://doi.org/10.1017/S0031182024000143.

Low heat tolerance and high desiccation resistance in nocturnal bees and implications for nocturnal pollination under climate change - (Peer Reviewed Journal)
Gonzalez, V.H., Manweiler, R., Smith, A.R., Oyen, K.J., Cardona, D., Wcislo, W.T. 2023. Low heat tolerance and high desiccation resistance in nocturnal bees and implications for nocturnal pollination under climate change. Scientific Reports. 13, 22320. https://doi.org/10.1038/s41598-023-49815-6.

Structural definition of babesial RAP-1 proteins identifies a novel protein superfamily across Apicomplexa - (Peer Reviewed Journal)
Isidro, H., Suarez, C.E. 2023. Structural definition of babesial RAP-1 proteins identifies a novel protein superfamily across Apicomplexa. Scientific Reports. 13:22330. https://doi.org/10.1038/s41598-023-49532-0.

An assessment of equine veterinarian knowledge and perceptions of ticks and tick-borne diseases in the United States to inform continuing education needs - (Peer Reviewed Journal)
Machtinger, E.T., Smarsh, D.N., Kenny, L.B., Poh, K.C., Orr, E.L., Kirkland, B.G., Springer, H.R. 2023. An assessment of equine veterinarian knowledge and perceptions of ticks and tick-borne diseases in the United States to inform continuing education needs. Equine Veterinary Journal. https://doi.org/10.1111/eve.13931.

Impact of Equine and Camel piroplasmosis in Egypt: how much do we know about the current situation? - (Peer Reviewed Journal)
Elsawy, B.S., Mahmoud, M.S., Suarez, C.E., Alzan, H.F. 2023. Impact of Equine and Camel piroplasmosis in Egypt: how much do we know about the current situation? Pathogens. 12(11):1318. https://doi.org/10.3390/pathogens12111318.

Experimental infection of non-immunosuppressed and immunosuppressed goats reveals differential pathogenesis of Babesia aktasi n. sp. - (Peer Reviewed Journal)
Ozubek, S., Ulucesme, M.C., Bastos, R.G., Alzan, H.F., Laughery, J.M., Suarez, C.E., Aktas, M. 2023. Experimental infection of non-immunosuppressed and immunosuppressed goats reveals differential pathogenesis of Babesia aktasi n. sp. Frontiers in Cellular and Infection Microbiology. https://doi.org/10.3389/fcimb.2023.1277956.

An integrative framework for tick management: The need to connect wildlife science, one health, and interdisciplinary perspectives - (Peer Reviewed Journal)
Machtinger, E.T., Poh, K.C., Pesapane, R., Tufts, D.M. 2023. An integrative framework for tick management: The need to connect wildlife science, one health, and interdisciplinary perspectives. Insect Science. https://doi.org/10.1016/j.cois.2023.101131.

Egg-hatching success is influenced by the time of thermal stress in four hard tick species - (Peer Reviewed Journal)
Oluwaseun, A.M., Oyen, K.J., Davies, B., Finch, G., Piller, B.D., Harmeyer, A.A., Wendeln, K., Perretta, C., Rosendale, A.J., Benoit, J.B. 2023. Egg-hatching success is influenced by the time of thermal stress in four hard tick species. Journal of Medical Entomology. https://doi.org/10.1093/jme/tjad142.

A comparison of European deer keds (Diptera: Hippoboscidae: Lipoptena cervi (Linnaeus)) and blacklegged ticks (Ixodida: Ixodidae: Ixodes scapularis say) on elk (Cervus canadensis (Erxleben)) and white-tailed deer (Odocoileus - (Peer Reviewed Journal)
Skvarla, M.J., Poh, K.C., Norman, C., Struckhoff, D.E., Machtinger, E. 2023. A comparison of European deer keds (Diptera: Hippoboscidae: Lipoptena cervi (Linnaeus)) and blacklegged ticks (Ixodida: Ixodidae: Ixodes scapularis say) on elk (Cervus canadensis (Erxleben)) and white-tailed deer (Odocoileus virgunianus (Zimmermann, 1780) in Pennsylvania. Journal of Entomological Society of Ontario. 154.

Differential burdens of blacklegged ticks (Ixodes scapularis) on sympatric rodent hosts - (Peer Reviewed Journal)
Brown, J.E., Tiffin, H.S., Pagac, A., Poh, K.C., Evans, J.R., Miller, T.M., Herrin, B.H., Sutherland, C., Machtinger, E.T. 2023. Differential burdens of blacklegged ticks (Ixodes scapularis) on sympatric rodent hosts. Journal of Vector Ecology. 49(1):44-52. https://doi.org/10.52707/1081-1710-49.1.44.

Vaccination of cattle with the Babesia bovis sexual-stage protein HAP2 abrogates parasite transmission by Rhipicephalus microplus ticks - (Peer Reviewed Journal)
Silva, M.G., Bastos, R.G., Laughery, J.M., Alzan, H.F., Rathinasamy, V.A., Cooke, B.M., Suarez, C.E. 2023. Vaccination of cattle with the Babesia bovis sexual-stage protein HAP2 abrogates parasite transmission by Rhipicephalus microplus ticks. NPJ Vaccines. Vaccines 8, 140. https://doi.org/10.1038/s41541-023-00741-8.

First report of an outbreak of “Q” fever in an abattoir from Argentina - (Peer Reviewed Journal)
Cardillo, N.M., Bastos, R.G., Garcia, A., Perez, R., Garcia, E., Lloveras, S., Suarez, C.E. 2023. First report of an outbreak of “Q” fever in an abattoir from Argentina. Zoonoses and Public Health. https://doi.org/10.1111/zph.13077.

Vaccination with an in vitro culture attenuated Babesia bovis strain safely protects highly susceptible adult cattle against acute bovine babesiosis - (Peer Reviewed Journal)
Bastos, R.G., Capelli-Peixoto, J., Laughery, J.M., Suarez, C.E., Ueti, M.W. 2023. Vaccination with an in vitro culture attenuated Babesia bovis strain safely protects highly susceptible adult cattle against acute bovine babesiosis. Frontiers in Immunology. 14. Article 1219913. https://doi.org/10.3389/fimmu.2023.1219913.

Local influence of floral resource attributes on urban bumble bee foraging activity - (Peer Reviewed Journal)
Pfeiffer, V.W., Zhu, J., Poh, K.C., Sibernagel, J. 2023. Local influence of floral resource attributes on urban bumble bee foraging activity. Frontiers in Sustainable Cities. 5. Article 1103721. https://doi.org/10.3389/frsc.2023.1103721.

Dielectric characterization of Babesia bovis using the dielectrophoretic crossover frequency - (Peer Reviewed Journal)
Oladokun, R., Adekanmbi, E., Ueti, M.W., Srivastava, S. 2023. Dielectric characterization of Babesia bovis using the dielectrophoretic crossover frequency. Electrophoresis. 44(11-12):988-1001. https://doi.org/10.1002/elps.202200263.

Spherical body protein 4 from Babesia bigemina: A novel gene that contains conserved B-cell epitopes and induces cross-reactive neutralizing antibodies in Babesia ovata - (Peer Reviewed Journal)
Mosqueda, J., Hernandez-Silva, D.J., Ueti, M.W., Cruz-Reséndiz, A., Marquez-Cervantez, R., Valdez-Espinoza, U.M., Dang-Trinh, M., Nguyen, T., Camacho-Nuez, M., Mercado-Uriostegui, M.A., Aguilar-Tipacamú, G., Ramos-Aragon, J.A., Hernandez-Ortiz, R., Kawazu, S., Igarashi, I. 2023. Spherical body protein 4 from Babesia bigemina: A novel gene that contains conserved B-cell epitopes and induces cross-reactive neutralizing antibodies in Babesia ovata. Pathogens. 12(3). Article 495. https://doi.org/10.3390/pathogens12030495.

Cross-sectional analysis of Piroplasma species-infecting camel (Camelus dromedaries) in Egypt using a multipronged molecular diagnostic approach - (Peer Reviewed Journal)
Mahdy, O.A., Nassar, A.M., Elsawy, B.S., Alzan, H.F., Kandil, O.M., Mahmoud, M.S., Suarez, C.E. 2023. Cross-sectional analysis of Piroplasma species-infecting camel (Camelus dromedaries) in Egypt using a multipronged molecular diagnostic approach. Frontiers in Veterinary Science. 10. Article 1178511. https://doi.org/10.3389/fvets.2023.1178511.

Degrade to survive: The intricate world of piroplasmid proteases - (Peer Reviewed Journal)
Florin-Christensen, M., Sojka, D., Ganzinelli, S., Šnebergerová, P., Suarez, C.E., Schnittger, L. 2023. Degrade to survive: The intricate world of piroplasmid proteases. Trends in Parasitology. 39(7):532-546. https://doi.org/10.1016/j.pt.2023.04.010.

Topical permethrin may increase blacklegged tick (Ixodes scapularis) repellency but is associated with cutaneous irritation in horses - (Peer Reviewed Journal)
Poh, K.C., Cole, Z.T., Smarsh, D.N., Springer, H.R., Kelly, K., Kenny, L.B., Machtinger, E.T. 2023. Topical permethrin may increase blacklegged tick (Ixodes scapularis) repellency but is associated with cutaneous irritation in horses. American Journal of Veterinary Research. 84(4). https://doi.org/10.2460/ajvr.22.10.0176.

Differential paired stage-specific expression of Babesia bovis cysteine-rich GCC2/GCC3 domain family proteins (BboGDP) during development within Rhipicephalus microplus - (Peer Reviewed Journal)
Hussein, H.E., Johnson, W.C., Ueti, M.W. 2023. Differential paired stage-specific expression of Babesia bovis cysteine-rich GCC2/GCC3 domain family proteins (BboGDP) during development within Rhipicephalus microplus. Parasites & Vectors. 16. Article 16. https://doi.org/10.1186/s13071-022-05628-6.

Comparison of high throughput RNA sequences between Babesia bigemina and Babesia bovis revealed consistent differential gene expression that is required for the Babesia life cycle in the vertebrate and invertebrate hosts - (Peer Reviewed Journal)
Capelli-Peixoto, J., Saelao, P., Johnson, W.C., Kappmeyer, L.S., Reif, K.E., Masterson, H.E., Taus, N.S., Suarez, C.E., Brayton, K.A., Ueti, M.W. 2022. Comparison of high throughput RNA sequences between Babesia bigemina and Babesia bovis revealed consistent differential gene expression that is required for the Babesia life cycle in the vertebrate and invertebrate hosts. Frontiers in Cellular and Infection Microbiology. 12. Article 1093338. https://doi.org/10.3389/fcimb.2022.1093338.

Neotropical stingless bees display a strong response in cold tolerance with changes in elevation - (Peer Reviewed Journal)
Gonzalez, V.H., Oyen, K.J., Nydia, V., Rodulfo, O. 2022. Neotropical stingless bees display a strong response in cold tolerance with changes in elevation. Conservation Physiology. 10:(1). https://doi.org/10.1093/conphys/coac073.

Identification of novel immune correlates of protection against acute bovine babesiosis by superinfecting cattle with in vitro culture attenuated and virulent Babesia bovis strains - (Peer Reviewed Journal)
Bastos, R.G., Laughery, J.M., Ozubek, S., Alzan, H.F., Taus, N.S., Ueti, M.W., Suarez, C.E. 2022. Identification of novel immune correlates of protection against acute bovine babesiosis by superinfecting cattle with in vitro culture attenuated and virulent Babesia bovis strains. Frontiers in Immunology. 13. Article 1045608. https://doi.org/10.3389/fimmu.2022.1045608.

Thrombospondin-related anonymous protein (TRAP) family expression by Babesia bovis life stages within the mammalian host and tick vector - (Peer Reviewed Journal)
Masterson, H.E., Taus, N.S., Johnson, W.C., Kappmeyer, L.S., Capelli-Peixoto, J., Hussein, H.E., Mousel, M.R., Hernandez-Silva, D.J., Laughery, J.M., Mosqueda, J., Ueti, M.W. 2022. Thrombospondin-related anonymous protein (TRAP) family expression by Babesia bovis life stages within the mammalian host and tick vector. Microorganisms. 10(11). Article 2173. https://doi.org/10.3390/microorganisms10112173.

OMIP-085: Cattle B-cell phenotyping by an 8-color panel - (Peer Reviewed Journal)
Roos, E.O., Bonnet-Di Placido, M., Mwangi, W., Moffat, K., Fry, L.M., Waters, R., Hammond, J.A. 2022. OMIP-085: Cattle B-cell phenotyping by an 8-color panel. Cytometry Journal. 103(1):12-15. https://doi.org/10.1002/cyto.a.24683.

A transfected Babesia bovis parasite line expressing eGFP is able to complete the full life cycle of the parasite in mammalian and tick hosts - (Peer Reviewed Journal)
Johnson, W.C., Hussein, H.E., Capelli-Peixoto, J., Laughery, J.M., Taus, N.S., Suarez, C.E., Ueti, M.W. 2022. A transfected Babesia bovis parasite line expressing eGFP is able to complete the full life cycle of the parasite in mammalian and tick hosts. Pathogens. 11(6). Article 623. https://doi.org/10.3390/pathogens11060623.

All for one health and one health for all: Considerations for successful citizen science projects conducting vector surveillance from animal hosts - (Peer Reviewed Journal)
Poh, K.C., Evans, J.R., Skvarla, M.J., Machtinger, E.T. 2022. All for one health and one health for all: Considerations for successful citizen science projects conducting vector surveillance from animal hosts. Insects. 13(6). Article 492. https://doi.org/10.3390/insects13060492.

Lactate dehydrogenase as a potential therapeutic drug target to control Babesia bigemina - (Peer Reviewed Journal)
He, L., Bastos, R.G., Yu, L., Laughery, J.M., Suarez, C.E. 2022. Lactate dehydrogenase as a potential therapeutic drug target to control Babesia bigemina. Frontiers in Cellular and Infection Microbiology. 12. Article 870852. https://doi.org/10.3389/fcimb.2022.870852.

Unraveling the complexity of the rhomboid serine protease 4 family of Babesia bovis by bioinformatics and experimental studies - (Peer Reviewed Journal)
Gallenti, R., Hussein, H.E., Alzan, H.F., Suarez, C.E., Ueti, M.W., Asurmendi, S., Benitez, D., Araujo, F.R., Rolls, P., Sibeko-Matjila, K., Schnittger, L., Florin-Christensen, M. 2022. Unraveling the complexity of the rhomboid serine protease 4 family of Babesia bovis by bioinformatics and experimental studies. Pathogens. 11(3) Article 344. https://doi.org/10.3390/pathogens11030344.

Clinical progression of Theileria haneyi in splenectomized horses reveals decreased virulence compared to Theileria equi - (Peer Reviewed Journal)
Sears, K.P., Knowles, D.P., Fry, L.M. 2022. Clinical progression of Theileria haneyi in splenectomized horses reveals decreased virulence compared to Theileria equi. Pathogens. 11(2). Article 254. https://doi.org/10.3390/pathogens11020254.

A culture-adapted strain of Babesia bovis has reduced subpopulation complexity and is unable to complete its natural life cycle in ticks - (Peer Reviewed Journal)
Alzan, H.F., Bastos, R.G., Laughery, J.M., Scoles, G.A., Ueti, M.W., Johnson, W.C., Suarez, C.E. 2022. A culture-adapted strain of Babesia bovis has reduced subpopulation complexity and is unable to complete its natural life cycle in ticks. Frontiers in Cellular and Infection Microbiology. 12. Article 827347. https://doi.org/10.3389/fcimb.2022.827347.

Identification of CCp5 and FNPA as novel non-canonical members of the CCp protein family in Babesia bovis - (Peer Reviewed Journal)
Ozubek, S., Alzan, H.F., Bastos, R.G., Laughery, J.M., Suarez, C.E. 2022. Identification of CCp5 and FNPA as novel non-canonical members of the CCp protein family in Babesia bovis. Frontiers in Veterinary Science. 9. Article 833183. https://doi.org/10.3389/fvets.2022.833183.