Location: Sustainable Perennial Crops Laboratory
Project Number: 8042-21000-303-014-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Jul 1, 2022
End Date: Jun 30, 2027
Objective:
The objective of this agreement is to support research through collaborations that provide high throughput next generation genotyping by sequencing services for tropical and semitropical plants; phytopathogenic fungi and bacteria; plant insect pests and their associated host plants. The data generated through the collaboration will be used in genetic diversity analysis. The parties will work to develop appropriate DNA and cDNA libraries from ARS provided materials. The Cooperator will provide sequencing, SNP analysis and data processing to ARS in the form of usable sequencing and SNP data as well as providing the raw data.
Approach:
The Cooperator under ARS direction will produce appropriate sequencing libraries for genotyping by sequencing and conduct high throughput sequencing. ARS will provide the Cooperator with isolated sample material, DNA and or RNA. The Cooperators will conduct initial and comparison analysis and will send the data to ARS or to a third party designated by ARS. The parties will work together to develop new methods of genotyping and germplasm evaluation
