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ARS Home » Pacific West Area » Hilo, Hawaii » Daniel K. Inouye U.S. Pacific Basin Agricultural Research Center » Tropical Pest Genetics and Molecular Biology Research Unit » Research » Research Project #444137

Research Project: Develop Molecular Diagnostic Tools to Determine Strain and Mating Status of Fruit Fly Incursions

Location: Tropical Pest Genetics and Molecular Biology Research Unit

Project Number: 2040-22430-028-024-I
Project Type: Interagency Reimbursable Agreement

Start Date: Aug 1, 2023
End Date: Sep 29, 2025

Objective:
Develop molecular diagnostic tools to determine strain and mating status of fruit fly incursions Year 2 of 2.

Approach:
1. Select candidate autosomal strain-specific and sex-linked markers. 2. Develop diagnostic assay discriminating males from females and GSS and wild. 3. Perform experimental crosses to validate diagnostic assay. 4. Develop methods to quantify mating of wild females to GSS males vs wild males.