Location: Dale Bumpers National Rice Research Center
Project Number: 6028-21000-012-011-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Jun 1, 2023
End Date: May 31, 2024
Objective:
To investigate distribution, relatedness and pathogenicity of Pantoea in rice, corn, peanut in Arkansas, Texas, and Oklahoma.
Approach:
The collaborator at Texas A&M AgriLife Extension, Amarillo, Texas, will perform surveys in corn-producing counties, Potter, Dallam, Hartley, Sherman, Moore, Hutchinson, Hansford, and Oldham Counties in Texas for Late Season Decline, and in peanut production fields for Bacterial Early-seedling Decline Disease Pantoea pathogens. This work will include 1) Isolation of corn and peanut Pantoea from symptomatic plants as well as from peanut seeds; 2) Validation of Pantoea status of isolates using 16S rRNA primers and analysis of sequence data; 3) Performing additional PCR and sequencing of 1-2 more housekeeping genes with specific primers; and 4) Characterization of isolates using multi-locus sequence-based typing with at least two housekeeping genes.
