Location: Foreign Animal Disease Research
Project Number: 3022-32000-064-037-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Jun 1, 2024
End Date: May 31, 2026
Objective:
To improve our understanding of B cell diversity and specificity, we propose to establish a pipeline that integrates single-cell RNA and B cell receptor (BCR) sequencing of B cells from PBMCs samples of both naïve and infected animals. A critical aspect of this work will involve the optimization of B cell enrichment from various tissues, followed by the creation of a bioinformatics and machine learning framework to decode the B cell repertoires and get paired antibody sequences. This project has the potential to identify the diversity and specificity of B cells, the tissue-specific immune responses and B cell maturation. These insights will significantly contribute to the rational design of vaccines and other effective immunological interventions.
Approach:
1. Establish a pipeline for enrichment of live B cell populations from livestock animals.
2. Establish a pipeline for single B-cell sequencing.
3. Establish a pipeline for bioinformatics and machine learning framework to decode B cell repertoires.
