Location: Genetic Improvement for Fruits & Vegetables Laboratory
Project Number: 8042-21000-023-001-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Sep 1, 2023
End Date: Aug 31, 2024
Objective:
Research is required to understand the interactions between host plants, insect vectors and false blossom diseases to implement effective disease and insect control programs.
1) Perform transcriptome analyses (RNAseq) from herbivore treated and untreated wild and cultivated blueberry germplasm
2) Perform experiments to characterize insect-vectored transmission of false blossom disease in cranberry
Approach:
1. Total RNA will be extracted from wild (diploid and tetraploid) and cultivated (tetraploid) blueberry accessions.
2. RNAseq will be performed using standard procedures (mRNA enrichment, cDNA synthesis, next-generation sequencing).
3. qRT-PCR assays will be designed and performed to confirm target gene expression (based on RNAseq data).
4. ARS will determine the species of leaf hopper that can acquire and transmit the disease through controlled feeding experiments using nymphs and adults of sharp-nosed and blunt-nosed leafhoppers.
5. ARS will test plant resistance in our cranberry germplasm collection through controlled feeding experiments using the vector(s) identified in approach 4 (above).
