Location: Plant Stress and Germplasm Development Research
Project Number: 3096-21000-024-001-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Sep 1, 2023
End Date: Sep 30, 2024
Objective:
1. To determine the whole genome sequence of sorghum isolines with variant msd1/msd2 allele in several genetic backgrounds.
2. To analyze plant hormone levels in sorghum isolines with variant msd1/msd2 allele in several genetic backgrounds at two stages of development.
3. Perform global gene expression by RT PCR for specific gene(s)of interest.
4. Conduct bioinformatic analyses of whole genome sequence and support in final anlaysis and deposit of DNA sequence to appropriate database.
Approach:
Whole genome sequencing & associated genomic DNA extraction of leaf samples from sorghum isolines will be performed at Center for BIotechnology & Genomics, TTU, with Illumina NovaSeq 6000, by cooperator. Hormone levels with focus on jasmonic acid and derivatives from tissue samples of sorghum isolines with variant msd1/msd2 allele in several genetic backgrounds at two stages of development will be performed using Vanquish UHPLC by cooperator. Bioinformatic analyses using appropriate alignment software/utilities and support in final anlaysis and deposit of DNA sequence to appropriate database will be provided by cooperator. The research conducted will provide much needed genomic and metabolomic information to CRIS project. Cooperative acitvities will lead to new DNA marker assay and publication of articles for dissemination of infomartion to stakeholders.
