Location: Diet, Genomics and Immunology Laboratory
Project Number: 8040-10700-006-000-D
Project Type: In-House Appropriated
Start Date: Mar 20, 2024
End Date: Mar 19, 2029
Objective:
Objective 1: Determine the impact of species and roasting on composition of coffee and determine potential effects of coffee composition on nonalcoholic fatty liver disease (NAFLD) and bacteria-induced intestinal infection. [NP107, C3, PS3B, C4, PS4A].
Sub-Objective 1.A: To determine the impact of species and roasting on the composition of coffee.
Sub-Objective 1.B: To investigate the potential effects of coffee compounds and composition differences on bacteria-induced intestinal infection.
Sub-Objective 1.C: To investigate the potential effects of coffee with different compound compositions on nonalcoholic fatty liver disease (NAFLD).
Objective 2. Determine the impact of genetics and growing conditions on composition of microgreens and whether the difference in microgreen composition affects NAFLD and bacteria-induced intestinal infection. [NP107, C3, PS3B, C4, PS4A].
Sub-Objective 2.A. To determine the effect of different species of microgreens on NAFLD and bacteria-induced intestinal infection.
Sub-Objective 2.B. To determine the effects of microgreens grown in different growing conditions on intestinal infection and development of NAFLD.
Approach:
We propose to use coffee and Brassica microgreens as food models to address the Objectives. The hypotheses related to health end points will be tested using a rodent Cr infection model and a high-fat diet-induced None Alcoholic Fatty Liver Disease (NAFLD) to assess 1) whether differences in profiles of bioactive compounds arise from the impact of species/genetics and growing/processing conditions and 2) whether these differences lead to differential effects on intestinal infection and development of NAFLD. Molecular and pathological markers for infection, inflammation, lipid metabolism and NAFLD will be determined to assess the effcts of treatments. Metagenomics analysis will be used to elucidate the effects of diet on the gut micrbiome and correlation with health endpoints. Addtionally, trascriptomics, proteomics and metabolomicds analysis of tissues, plasma will be performed to elcucidate bioavailaility of specific nutrients and to provide in-depth detail of mechnisms of action exterted by coffee and microgreens.
