Location: Corn Insects and Crop Genetics Research
Project Number: 5030-21220-007-006-R
Project Type: Reimbursable Cooperative Agreement
Start Date: Jan 1, 2024
End Date: Dec 31, 2024
Objective:
This 2nd year project will continue to release cultivars and germplasm with resistance to one or more of these pathogens as well as identify and characterize the candidate genes for development of ‘perfect’ molecular markers for rapid and precise incorporation of the targeted resistance into U.S. germplasm and ultimately varieties that farmers can grow. This work continues to be important as pathogens evolve to evade plant defense responses.
Approach:
1. Cooperators will continue to develop new and improved soybean varieties and germplasm with enhanced resistance traits.
2. Cooperators and ARS PI will use mapping, whole genome expression analyses to identify the specific gene(s) contributing to resistance and determine how resistance is regulated.
3. Cooperators will use Virus-induced gene silencing (VIGS) and CRIPSR/Cas9 technologies to confirm candidate genes.
4. ARS PI will use VIGS coupled with RNA-seq to confirm candidate genes and the molecular networks they control.
