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ARS Home » Southeast Area » Stoneville, Mississippi » Genomics and Bioinformatics Research » Research » Research Project #446186

Research Project: The Development of a high Throughput Salmonella Pathogenic Screening Assay

Location: Genomics and Bioinformatics Research

Project Number: 6066-21310-006-034-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Aug 15, 2024
End Date: Aug 14, 2027

Objective:
Develop a high throughput Salmonella pathogenicity assay by modifying the gentamicin protection assay for next generation sequencing. The Cooperator will collect and analyze data for the development of a single-cell DNAseq based version of the gentamicin protection assay.

Approach:
A high throughput version of the gentamicin protection assay will be designed based on Single cell DNA Sequencing for screening the pathogen city of hundreds of Salmonella strains simultaneously.