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ARS Home » Midwest Area » Ames, Iowa » Plant Introduction Research » Research » Research Project #446279

Research Project: Exploring the Microbiome in Exotic Adapted Maize Germplasm for the Effects of Selection on Nitrogen Use Efficiency

Location: Plant Introduction Research

Project Number: 5030-21000-074-005-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Aug 1, 2024
End Date: Jul 31, 2025

Objective:
Utilizing doubled haploid (DH) and released lines from the Germplasm Enhancement of Maize (GEM) Project, explore the effects of the microbiome for the effects of selection on nitrogen use efficiency (NUE).

Approach:
GEM DH and released lines will be planted in Lincoln, Nebraska. Prior to planting, soil samples are taken and sequenced. Sequence data will be analyzed to establish the microbiome baseline. At key periods throughout the growing season, flowering and senescence, additional soil samples will be taken from each plot and analyzed to determine the microbiome present for each GEM DH and released line. Microorganisms present in the microbiome and are associated with nitrogen processes will be scrutinized to determine differences in NUE and other environmental impact related traits.