Location: Foreign Animal Disease Research
Project Number: 3022-32000-064-044-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Aug 1, 2024
End Date: Jul 31, 2025
Objective:
Previous research on the stability of Adenovirus type 5 (Ad5)-vectored expression systems (i.e., Ad5-Gluciferase) using a specific formulation resulted in stable luminescent signal both in vitro and in vivo. The objective of this collaborative effort is to conduct an in vivo proof-of-concept study to evaluate the efficacy of a self-boosting microneedle formulation using the Ad5-Foot and Mouth Disease (FMD) vectored expression system. Additionally, this study aims to evaluate the immunological efficiency of this novel delivery system.
Approach:
1. Fabricating and characterizing the simple version of the Ad5-FMD vaccine microneedle formulation with immediate release, sufficient vaccine dose, mechanical strength and stability in vitro.
2. Characterizing the immunogenic efficiency and safety of the simple version microneedles in vivo.
