Location: Crop Production and Protection
Project Number: 0500-00053-005-172-G
Project Type: Grant
Start Date: May 1, 2024
End Date: Apr 30, 2026
Objective:
1. Measure aggressiveness and amount of DON produced for 150 Fg isolates from the NA1
population, for the purpose of performing genome-wide association studies (GWAS) of these
traits.
2. Expand the set of genetic markers segregating in this sample and available for GWAS analysis.
Approach:
We will perform additional years of greenhouse head inoculation experiments on susceptible and resistant wheat to refine our phenotyping of the Fg isolates used in our FY22 USWBSI project. We will prepare barcoded DNA libraries with an inexpensive and high-throughput protocol and then perform whole genome sequencing of pooled libraries.