Location: Healthy Body Weight Research
Project Number: 3062-51000-057-026-A
Project Type: Cooperative Agreement
Start Date: Sep 15, 2024
End Date: Sep 15, 2025
Objective:
USDA-ARS (Grand Forks Human Nutrition Center) is focused on improving our understanding of the health roles of foods and physical activity. USDA-ARS is interested in finding plasma markers at the protein level that may be downstream from specific genetic variants suspected to play a role in the reinforcing value of exercise (based on the hypothesis that some people have a stronger desire to exercise). The key objective of this study is to generate a comprehensive proteomics profile of plasma samples from exercise-trained and untrained adult participants who completed resting and acute medium and high-intensity aerobic exercise conditions. Serum samples were collected before, immediately after, and 30, 60, and 90 minutes after the rest and exercise conditions.
Approach:
The Cooperator will apply a platform that offers integrated proteomics solutions for novel biomarker discovery. Human plasma samples will be analyzed using a patented Hyper Reaction Monitoring (HRM) mass spectrometry, an advanced Data Independent Acquisition (DIA)-based protein quantification technology. The platform enables the deepest unbiased profiling of tissue and biofluid proteomes on a large scale. The platform searches the complete proteome to quantify thousands of the most relevant proteins, including an unlimited number of proteoforms. The generated data are highly reproducible. This approach was employed for a previous USDA-ARS-GFHNRC study and utilizing he same Cooperator allows for across -study comparison and combining of data.
