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ARS Home » Southeast Area » Raleigh, North Carolina » Soybean and Nitrogen Fixation Research » Research » Research Project #447407

Research Project: Determining the Causes of Declining Test Weight of Soybean and Utilizing Breeding to Increase Test Weight

Location: Soybean and Nitrogen Fixation Research

Project Number: 6070-21220-070-071-A
Project Type: Cooperative Agreement

Start Date: Oct 1, 2024
End Date: Sep 30, 2025

Objective:
Determine the relation of test weight to seed size, seed quality, protein and oil content, sucrose concentration, amino acid content and yield.

Approach:
The Cooperator will evaluate test weight on the 2025 Northern Uniform Soybean Tests (NUST) (MG 00, 0, 1) grown at the Minnesota locations. The MG 00-I NUST trials typically include a total of 130 advanced breeding lines, which are grown at between two and three MN locations in randomized block designs with three replications. This amounts to a total of approximately 975 plots. Samples from these plots will also be screened using NIR to evaluate comprehensive seed composition, including protein, oil, and carbohydrates. Seed weight will also be determined. These same measurements will also be collected on the Minnesota Statewide Variety trials, which include both commercial and public varieties. Approximately 150 varieties are evaluated in these trials each year, with trials occurring across three Minnesota locations in each maturity zone. These activities will provide a solid foundation of knowledge in terms of variation in test weight across varieties in the northern U.S., and the main underlying factors driving variation in test weight. All lines entered into NUST are being genotyped with high-density molecular markers. The Cooperator will combine phenotypic data with genomic data to map QTL, and develop genomic prediction models for test weight.